Taxanes from Shells and Leaves of <i>Corylus avellana</i>

Ottaggio, Laura; Bestoso, Federica; Armirotti, Andrea; Balbi, Alessandro; Damonte, Gianluca; Mazzei, Mauro; Sancandi, Monica; Miele, Mariangela

doi:10.1021/np0704046

Cited by 65 publications

(32 citation statements)

References 13 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Potential paclitaxel biosynthetic gene homologs with identity > 30% to the 13 reported paclitaxel biosynthetic genes were found in the paclitaxel-producing hazel [24,25]. The most conserved genes were GGPS and PAM with amino acid identities of 62% and 63%, respectively (in Additional file 3: Data S4).…”

Section: Resultsmentioning

confidence: 99%

Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431

et al. 2014

View full text Add to dashboard Cite

BackgroundPaclitaxel (Taxol™) is an important anticancer drug with a unique mode of action. The biosynthesis of paclitaxel had been considered restricted to the Taxus species until it was discovered in Taxomyces andreanae, an endophytic fungus of T. brevifolia. Subsequently, paclitaxel was found in hazel (Corylus avellana L.) and in several other endophytic fungi. The distribution of paclitaxel in plants and endophytic fungi and the reported sequence homology of key genes in paclitaxel biosynthesis between plant and fungi species raises the question about whether the origin of this pathway in these two physically associated groups could have been facilitated by horizontal gene transfer.ResultsThe ability of the endophytic fungus of hazel Penicillium aurantiogriseum NRRL 62431 to independently synthesize paclitaxel was established by liquid chromatography-mass spectrometry and proton nuclear magnetic resonance. The genome of Penicillium aurantiogriseum NRRL 62431 was sequenced and gene candidates that may be involved in paclitaxel biosynthesis were identified by comparison with the 13 known paclitaxel biosynthetic genes in Taxus. We found that paclitaxel biosynthetic gene candidates in P. aurantiogriseum NRRL 62431 have evolved independently and that horizontal gene transfer between this endophytic fungus and its plant host is unlikely.ConclusionsOur findings shed new light on how paclitaxel-producing endophytic fungi synthesize paclitaxel, and will facilitate metabolic engineering for the industrial production of paclitaxel from fungi.

show abstract

Section: Resultsmentioning

confidence: 99%

Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Hazel (Corylus avellana L.) is an individual plant among angiosperms which has been reported to be able to produce Taxol and other taxanes [14,15]. The main benefit of producing taxanes by hazel cell cultures is that hazel is widely available, fast growing in vivo, and easier to cultivate in vitro than yew [15,16]. Synthesis of Taxol necessitates the attachment of the N-benzoyl-3-phenylisoserine side chain to the C-13 hydroxyl group of 10-deacetyl baccatin III (10-DABIII) and the acetylation of the 10b-hydroxyl position [17].…”

Section: Introductionmentioning

confidence: 99%

Effect of phenylalanine on Taxol production and antioxidant activity of extracts of suspension-cultured hazel (Corylus avellana L.) cells

et al. 2012

View full text Add to dashboard Cite

Taxol is produced by a few microorganisms and plants such as yew (Taxus sp.). Recent researches have shown that hazel (Corylus avellana L.) is also able to produce Taxol. In the present study, effects of different concentrations of phenylalanine (Phe) on the production of Taxol, antioxidant activity, and cytotoxic effects of extracts of suspension-cultured hazel cells were investigated. The cells were treated with different concentrations of Phe on day 7 of subculture and were harvested on day 14. The results showed that the amounts of Taxol and antioxidant activity were increased by increasing the phenylalanine supply. Interestingly, the cytotoxic effects of hazel cell extract were even stronger than that of pure Taxol (standard), suggesting hazel cell extract as a novel and suitable probe for treating human cancer. Application of phenylalanine to hazel cells exaggerates their effects.

show abstract

“…In addition to Taxus species, Corylus avellana L. (hazel) has been reported to produce paclitaxel (Ottaggio et al, 2008). Interestingly, an endophytic fungus isolated from Taxus and other species was found to produce paclitaxel as well, suggesting that fermentation of microorganisms could serve as an alternative approach for paclitaxel production (Mirjalili et al, 2012; Somjaipeng et al, 2015).…”

Section: Classical Plant-derived Mtasmentioning

confidence: 99%

Harnessing Plant Biodiversity for the Discovery of Novel Anticancer Drugs Targeting Microtubules

Xie

Zhou

2017

Front. Plant Sci.

View full text Add to dashboard Cite

The microtubule cytoskeleton plays a critical role in a wide range of cellular activities and has been shown to be a highly effective target for the treatment of human malignancies. Despite the recent focus on proteomics and high-throughput profiling, it is clear that analysis of plant extracts has yielded several highly efficacious microtubule-targeting agents (MTAs) currently in clinical use, as well as agents in the current pipeline with promising efficacy. To date, a large proportion of the world’s plant biodiversity remains untapped by the pharmaceutical industry, presenting a major opportunity for the discovery of novel pharmacologically active lead compounds. Because plants contain an astonishing array of structurally diverse molecules, they represent an ideal source for the discovery of novel MTA leads. To demonstrate the importance of searching for novel bioactive compounds across the plant kingdom, herein, we summarize the discovery and development of plant-derived MTAs and discuss the challenges associated with searching for novel bioactive compounds from plants. We propose potential solutions to these problems with the aim of facilitating further exploration and identification of novel MTAs from plant biodiversity.

show abstract

Taxanes from Shells and Leaves of Corylus avellana

Cited by 65 publications

References 13 publications

Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431

Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431

Effect of phenylalanine on Taxol production and antioxidant activity of extracts of suspension-cultured hazel (Corylus avellana L.) cells

Harnessing Plant Biodiversity for the Discovery of Novel Anticancer Drugs Targeting Microtubules

Contact Info

Product

Resources

About