Taxane analysis by high performance liquid chromatography–Nuclear magnetic resonance spectroscopy of Taxus species

Taxus ingredients in the red arils of Taxus baccata L. determined by HPLC‐MS/MS

“…Today, the analysis of Taxus ingredients is performed mostly by high performance liquid chromatography (HPLC) using photodiode array (PDA) detection and mass spectrometry (MS, MS/MS, laser desorption/ionisation) detection . Besides nuclear magnetic resonance (NMR) and fluorescence detection was described. Immunological methods and thin layer chromatography have declined in significance …”

“…Today, the analysis of Taxus ingredients is performed mostly by high performance liquid chromatography (HPLC) using photodiode array (PDA) detection [12][13][14][15][16][17][18][19][20][21][22][23][24][25] and mass spectrometry (MS, MS/MS, laser desorption/ionisation) detection. 10,21,[25][26][27][28][29][30][31] Besides nuclear magnetic resonance (NMR) 32,33 and fluorescence 34 detection was described.…”

A Photometric Screening Method for Dimeric Naphthylisoquinoline Alkaloids and Complete On-Line Structural Elucidation of a Dimer in Crude Plant Extracts, by the LC−MS/LC−NMR/LC−CD Triad

“…In different combinations, these hyphenated techniques have already proven their value for the analysis of complex matrixes. [20][21][22][23][24] The on-line elucidation of relative or absolute configurations of chiral molecules right from crude extracts, however, without the use of chiral chromatographic phases, is still a demanding task, which has been accomplished only rarely. [25][26][27][28][29] In this paper, we present a screening procedure for this pharmacologically promising class of dimeric naphthylisoquinoline alkaloids.…”

Hyphenated NMR Methods in Natural Products Research, Part 1: Direct Hyphenation

“…4). Other reported stopped-flow experiments deal with hop bitter acids [38], [65], starfish saponins [51], ecdysteroids of Silene otites (Caryophyllaceae) [39], [66], taxanes from Taxus species (Taxaceae) [67], metabolites from Anigozanthos species (Haemodoraceae) [68], [69], [70], lignans from Torreya jackii (Taxaceae) [71], extract of St. John's wort [72], Agromyces culture broth extract [73], a lignan glucoside from flax seed [74], flavonoids in Sorocea bomplandii (Moraceae) [75] and Trifolium pratense (Fabaceae) extract [76], protoberberine alkaloid fraction from Corydalis (Fumariaceae) cell cultures [77], [78], major metabolites of Aloe littoralis (Asphodelacaea) [79] (Fig. 5), flavonoid glycosides in transgenic tomatoes [80], sesame oil lignans [81], quinic acid derivatives from Fagara zanthoxyloides (Rutaceae) [82], betalain fraction from Hylocereus polyrhizus (Cactaceae) [83], phthalides from Ligusticum chuanxiong (Apiaceae) [84], and metabolites from several Chinese plants [85].…”