Tauroursodeoxycholic acid (TUDCA) enhanced intracytoplasmic sperm injection (ICSI) embryo developmental competence by ameliorating endoplasmic reticulum (ER) stress and inhibiting apoptosis

Abstract

Purpose The objective of this study was to examine the effect of tauroursodeoxycholic acid (TUDCA) on intracytoplasmic sperm injection (ICSI) embryos by evaluating endoplasmic reticulum (ER) stress, apoptosis, and embryo developmental competence in vitro and in vivo. Methods ER stress-associated genes and apoptosis-associated genes were measured and apoptosis index was analyzed. Embryo developmental competence was assessed in vitro and in vivo via the inner cell mass (ICM)/trophectoderm (TE) index, pregnancy and implantation rates, and birth rate. ResultsThe relative mRNA and protein expression of binding immunoglobulin protein (BIP) was significantly higher in the ICSI embryo group without TUDCA treatment (ICSI-C) than in the in vitro fertilization (IVF) group and in the ICSI embryo group with TUDCA treatment (200 μM) (ICSI-T), while TUDCA ameliorated ER stress in ICSI embryos. Embryos in the ICSI-C group showed a higher apoptosis index than those in the IVF group and ICSI-T group, and there was no significant difference between the IVF group and ICSI-T group. TUDCA can significantly improve ICSI embryo developmental competence in vitro and in vivo based on the ICM/TE index, pregnancy and implantation rates, and birth rate. Conclusion ICSI embryos manifested high ER stress and high apoptosis, while TUDCA ameliorated ER stress and reduced apoptosis in ICSI embryos. TUDCA can significantly improve the developmental competence of ICSI embryos in vitro and in vivo. This study provides a new idea for improving the efficiency of ICSI, and it will also have a positive effect on the development of assisted reproduction technologies for humans and other animals.

Microbial and metabolomic mechanisms mediating the effects of dietary inulin and cellulose supplementation on porcine oocyte and uterine development

“…In this study, the secondary bile acids deoxycholic acid and taurochenodeoxycholic acid were increased in DF 1.75 gilts compared with DF 1.0 gilts. Tauroursodeoxycholic acid was shown to facilitate DNA damage repair and improve early embryo development in pigs [ 55 ] and other mammals [ 56 ]. Additionally, DF intake also altered levels of other metabolites, including spermidine [ 57 ], 4-aminobenzoic acid [ 58 ] and ibuprofen [ 59 ] that are known to influence oocyte quality or reproductive function.…”

Endoplasmic reticulum stress-related neuroinflammation and neural stem cells decrease in mice exposure to paraquat

“…But, limited by the biological characteristics of SVZ, we only detected the ER stress and microglia in SGZ, which is a deficiency in our work. Another deficiency was the selectivity and specifically of TUDCA in inhibitory on ER stress [48][49][50][51] . Collectively, those results suggested that the increased ER stress in microglia might be a possible pathway for PQ-induced neuroinflammation and NSC impairment.…”