Taurolidine induces epithelial-mesenchymal transition via up-regulation of the transcription factor Snail in human pancreatic cancer cell lines

Hotz, Birgit; Erben, Ulrike; Arndt, Marco; Buhr, H. J.; Hotz, Hubert G.

doi:10.1007/s00384-014-1998-4

Cited by 1 publication

(1 citation statement)

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“…The substance is further effective against malignancy both, in vivo and in vitro [25][26][27][28][29]. Taurolidine curtails tumor blood supply by suppressing angiogenesis [29,30].…”

Section: Introductionmentioning

confidence: 99%

Taurolidine Sensitivity of Eryptosis, the Suicidal Erythrocyte Death

Fink

Bhuyan

Zacharopoulou

et al. 2018

Cell Physiol Biochem

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Background/Aims: The taurine derivative Taurolidine is effective against diverse bacteria and tumor growth. In the treatment of cancer, the substance is effective in part by triggering suicidal death or apoptosis of tumor cells. The Taurolidine-induced apoptosis involves mitochondria. Erythrocytes lack mitochondria but are nevertheless able to enter suicidal erythrocyte death or eryptosis, which is characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Signaling of eryptosis includes increase of cytosolic Ca²⁺ activity ([Ca²⁺]_i), oxidative stress and ceramide. The present study explores, whether Taurolidine induces eryptosis and, if so, which cellular mechanisms are involved. Methods: Phosphatidylserine exposure at the cell surface was estimated using annexin-V-binding, cell volume using forward scatter, [Ca²⁺]_i using Fluo3-fuorescence, reactive oxygen species (ROS) formation using 2’,7’-dichlorodihydrofuorescein (DCF)-dependent fluorescence, and ceramide abundance using specific antibodies. Results: A 48 hours exposure of human erythrocytes to Taurolidine (60 µg/ml) significantly enhanced the percentage of annexin-V-binding cells, significantly decreased forward scatter and significantly increased Fluo3-fluorescence and ceramide abundance, but not DCF-fluorescence. The effect of Taurolidine on annexin-V-binding was virtually abrogated by removal of extracellular Ca²⁺. Conclusion: Taurolidine triggers cell shrinkage and phospholipid scrambling of the erythrocyte cell membrane, an effect at least in part due to Ca²⁺ entry and paralleled by increase of ceramide abundance.

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“…The substance is further effective against malignancy both, in vivo and in vitro [25][26][27][28][29]. Taurolidine curtails tumor blood supply by suppressing angiogenesis [29,30].…”

Section: Introductionmentioning

confidence: 99%