Taurine inhibits Streptococcus uberis-induced NADPH oxidase-dependent neutrophil extracellular traps via TAK1/MAPK signaling pathways

Abstract

Neutrophil extracellular traps (NETs) are produced by neutrophil activation and usually have both anti-infective and pro-damage effects. Streptococcus uberis (S. uberis), one of the common causative organisms of mastitis, can lead to the production of NETs. Taurine, a free amino acid abundant in the organism, has been shown to have immunomodulatory effects. In this study, we investigated the molecular mechanisms of S. uberis-induced NETs formation and the regulatory role of taurine. The results showed that NETs had a disruptive effect on mammary epithelial cells and barriers, but do not significantly inhibit the proliferation of S. uberis. S. uberis induced NADPH oxidase-dependent NETs. TLR2-mediated activation of the MAPK signaling pathway was involved in this process. Taurine could inhibit the activation of MAPK signaling pathway and NADPH oxidase by modulating the activity of TAK1, thereby inhibiting the production of ROS and NETs. The effects of taurine on NADPH oxidase and NETs in S. uberis infection were also demonstrated in vivo. These results suggest that taurine can protect mammary epithelial cells and barriers from damage by reducing S. uberis-induced NETs. These data provide new insights and strategies for the prevention and control of mastitis.

WITHDRAWN: Gut microbiota aggravates neutrophil extracellular traps-induced pancreatic injury in hypertriglyceridemic pancreatitis

“…Taurine controls both PMA and HOCl-induced NETs release, and rescue neutrophils from programmed cell death46 . Taurine deactivates MAPK signaling pathway and NADPH oxidase that suppress NETs release47 . In addition, IL-17 is a potent inducer of NETosis, and IL-17 inhibitor reduced NETs formation48 .We found both IL-17 and NF-κB signaling pathways were activated in neutrophils from FMT-HTGP mice, which present considerations from the perspective of neutrophils recruitment and NETs formation in HTGP.Of note, we described disordered gut microbiota profiles in HTGP patients, and Bacteroides uniformis-derived taurine suppresses neutrophil infiltration and NETs which ameliorate pathological changes in HTGP.…”

Gut microbiota aggravates neutrophil extracellular traps-induced pancreatic injury in hypertriglyceridemic pancreatitis

“…Taurine confines both PMA and hypochlorous acid-induced NETs release, and rescues neutrophils from programmed cell death 48 . In addition, taurine deactivates MAPK signaling pathway and disrupts NADPH oxidase that reduce NETs formation 49 . We observed that FMT-HTGP activates IL-17 and NF-κB signaling pathways within neutrophils, and the increased levels of IL-17 and phosphorylation of NF-κB can be blocked by taurine supplementation.…”

Genetic polymorphisms in immune- and inflammation-associated genes and their association with bovine mastitis resistance/susceptibility

“…Genes involved in biological signaling pathways References bta04610:Complement and coagulation cascades C4A, C5, MASP2, A2M, CD46, CLU, MBL1, MBL2 (187,195); current analysis bta04145:Phagosome NCF1, NCF4, CD14, TLR4, MBL1, MBL2, TLR2 (196), current analysis bta05150:Staphylococcus aureus infection C4A, C5, MASP2, MBL1, MBL2 (187,196); current analysis bta04620:Toll-like receptor signaling pathway CXCL8, LBP, CD14, TLR4, TLR2 (187,195); current analysis bta04613:Neutrophil extracellular trap formation C5, NCF1, NCF4, HMGB1, TLR4, TLR2 current analysis; (197,198) Staphylococcus aureus can cause multiple forms of infections ranging from superficial skin infections to food poisoning and life-threatening infections.…”