Taurine induces autophagy and inhibits oxidative stress in mice Leydig cells

Yahyavy, Shokofeh; Valizadeh, Armita; Saki, Ghasem; Khorsandi, Layasadat

doi:10.5935/1518-0557.20190079

Cited by 10 publications

(9 citation statements)

References 26 publications

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“… 30 In addition, inhibition of autophagy could induce oxidative stress and reduce testosterone levels. 31 In the current study, TP significantly inhibited autophagy in MTLC-1 cells via downregulation of ATG7, ATG5 and LC3B, which was consistent with previous research. 32 Conversely, downregulation of miR-200a increased the expressions of LC3B, ATG7 and ATG5, but decreased the expression of p62 in TP-treated Leydig cells, thereby promoting cell autophagy.…”

Section: Discussionsupporting

confidence: 92%

<p>Downregulation of miR-200a Protects Mouse Leydig Cells Against Triptolide by Triggering Autophagy</p>

Miao

2020

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Background MicroRNAs play important roles in testicular development and spermatogenesis. Previous research has indicated that the level of miR-200a was significantly upregulated in patients with different spermatogenic impairments. However, the mechanism by which miR-200a regulated spermatogenic impairments remains unclear. Methods Leydig cells were treated with triptolide (TP) to mimic spermatogenic impairments. CCK-8 and flow cytometry were used to detect the proliferation and apoptosis in Leydig cells, respectively. In addition, Western blot assay was used to examine ATG7, ATG5, p62 protein levels in MLTC-1 cells. Results TP dose-dependently upregulated the expression of miR-200a in MLTC-1 cells. In addition, TP inhibited the proliferation of MLTC-1 cells via inducing apoptosis and oxidative stress; however, these phenomena were notably reversed by miR-200a antagomir. Furthermore, luciferase reporter assay identified that ATG7 was the direct binding target of miR-200a. TP treatment markedly inhibited the activation of autophagy in MLTC-1 cells via inhibition of ATG7. Conversely, downregulation of miR-200a significantly induced autophagy in TP-treated MLTC-1 cells by activation of ATG7. Meanwhile, the cell protective effects of miR-200a against TP were reversed by autophagy inhibitor 3MA, indicating that autophagy plays an important role. Conclusion These results indicated that downregulation of miR-200a could protect MLTC-1 cells against TP by inducing autophagy. Therefore, miR-200a might serve as a new therapeutic target for the treatment of male hypogonadism.

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Section: Discussionsupporting

confidence: 92%

<p>Downregulation of miR-200a Protects Mouse Leydig Cells Against Triptolide by Triggering Autophagy</p>

Miao

2020

DDDT

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“…TAU can increase the antioxidant level, membrane‐stabilising and motility of the spermatozoa (Yang et al., 2015). The beneficial impacts of the TAU on the Leydig cells have been reported in our previous study (Yahyavy et al., 2020). This study evaluated TAU impacts on DEHP‐induced apoptosis and oxidative stress and decreased testosterone secretion in the Leydig TM3 cell line.…”

Section: Introductionsupporting

confidence: 76%

“…TAU can increase the antioxidant level, membrane-stabilising and motility of the spermatozoa (Yang et al, 2015). The beneficial impacts of the TAU on the Leydig cells have been reported in our previous study (Yahyavy et al, 2020).…”

Section: Introductionsupporting

confidence: 53%

Taurine ameliorates cytotoxic effects of Di(2‐ethylhexyl) phthalate on Leydig cells

et al. 2021

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It has been revealed that di(2‐ethylhexyl)phthalate (DEHP) has toxic impacts on the male reproductive system. Taurine (TAU) is an amino acid with antioxidant property and beneficial impacts on the male reproductive system. In this study, protective impacts of Taurine (TAU) on DEHP‐induced Leydig TM3 cell toxicity were investigated. The cells exposed to DEHP (0.8 µmol) or TAU (100 mg/ml) for 24 hr. Cell viability (MTT assay), apoptosis, oxidative stress and testosterone level were examined. DEHP could significantly decrease the cell viability percentage, reduce testosterone level, increase apoptosis, elevate Bax/ Bcl‐2 ratio and enhance caspase‐3 and ‐9 activity in the TM3 cells. Additionally, DEHP significantly elevated malondialdehyde contents and reactive oxygen species levels. It also augmented superoxide dismutase and catalase activity in the Leydig cells. Co‐treatment of DEHP with TAU increased viability and testosterone level, while oxidative stress and apoptosis significantly reduced. TAU could decrease Bax/Bcl‐2 ratio and caspase‐3 and ‐9 activity in the DEHP‐intoxicated cells. Our results have clearly shown that TAU protects TM3 cells against oxidative stress and apoptosis induced by DEHP.

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“…Indeed, taurine ameliorates testicular damage in rats, through upregulation of anti-oxidant enzymes ( 276 ). Finally, the protective properties of taurine have been confirmed in murine testicular Leydig TM3 cells, preventing oxidative stress through activation of autophagy ( 277 ). Last but not least, the combination of curcumin and taurine exerts beneficial effect on Bisphenol A (BPA)-mediated testicular dysfunction in rats, though its inhibitory action on MDA levels and its possibility to upregulate anti-oxidant enzymes ( 278 ).…”

Section: The Anti-oxidant Properties Of Taurine In Various Toxic-mediated Insultsmentioning

confidence: 98%

Protective role of taurine against oxidative stress (Review)

et al. 2021

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Taurine is a fundamental mediator of homeostasis that exerts multiple roles to confer protection against oxidant stress. The development of hypertension, muscle/neuroassociated disorders, hepatic cirrhosis, cardiac dysfunction and ischemia/reperfusion are examples of some injuries that are linked with oxidative stress. The present review gives a comprehensive description of all the underlying mechanisms of taurine, with the aim to explain its anti-oxidant actions. Taurine is regarded as a cytoprotective molecule due to its ability to sustain normal electron transport chain, maintain glutathione stores, upregulate anti-oxidant responses, increase membrane stability, eliminate inflammation and prevent calcium accumulation. In parallel, the synergistic effect of taurine with other potential therapeutic modalities in multiple disorders are highlighted. Apart from the results derived from research findings, the current review bridges the gap between bench and bedside, providing mechanistic insights into the biological activity of taurine that supports its potential therapeutic efficacy in clinic. In the future, further clinical studies are required to support the ameliorative effect of taurine against oxidative stress. Contents1. Introduction 2. The role of taurine in homeostasis 3. The role of taurine against oxidative stress and its underlying molecular mechanisms 4. The beneficial effect of taurine against neuro-associated disorders 5. The anti-oxidant efficacy of taurine against cardiacassociated oxidative stress 6. The regulatory importance of taurine in ischemia and reperfusion 7. The anti-oxidant efficacy of taurine against muscle-associated disorders 8. The anti-oxidant efficacy of taurine against hepatic-associated stress 9. The anti-oxidant properties of taurine in various toxicmediated insults 10. Conclusions

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Taurine induces autophagy and inhibits oxidative stress in mice Leydig cells

Cited by 10 publications

References 26 publications

<p>Downregulation of miR-200a Protects Mouse Leydig Cells Against Triptolide by Triggering Autophagy</p>

<p>Downregulation of miR-200a Protects Mouse Leydig Cells Against Triptolide by Triggering Autophagy</p>

Taurine ameliorates cytotoxic effects of Di(2‐ethylhexyl) phthalate on Leydig cells

Protective role of taurine against oxidative stress (Review)

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