TAT-modified self-assembled cationic peptide nanoparticles as an efficient antibacterial agent

He, Bi; Ma, Shihua; Guo, Pei; He, Daohang

doi:10.1016/j.nano.2017.11.002

Cited by 55 publications

(48 citation statements)

References 32 publications

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“…The liposomes were determined for their hemolytic rate against rabbit red blood cell (rRBC) 37. Fresh rRBC were washed thrice with saline by centrifugation at 3000 rpm for 5 mins and were suspended in saline.…”

Section: Methodsmentioning

confidence: 99%

Preparation And Antibacterial Effects Of Carboxymethyl Chitosan-Modified Photo-Responsive Camellia Sapogenin Derivative Cationic Liposomes

Zhang¹,

Ye²,

Liu³

et al. 2019

IJN

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BackgroundBacterial resistance to antibiotics is a persistent and intractable problem. The sapogenin isolated from the seeds of Camellia oleifera can inhibit antibiotic-resistant bacteria after structural modification.PurposeThis study aims to improve sapogenin's antibacterial activity and avoid bacterial resistance based on nano-preparation with photo responsiveness.MethodsThe liposome shell material of carboxymethyl chitosan-phosphatidyl ethanolamine (CMC-PE) was prepared using amidation reaction, and photo-responsive cationic (PCC) liposomes containing Camellia sapogenin derivative (CSD) and photosensitizer pheophorbide-a were prepared by film dispersion method. Encapsulation efficiency, drug loading, zeta potential, particle size distribution, morphology and stability of the PCC liposomes were determined by HPLC, particle size analyzer, transmission electron microscopy (TEM) and fluorescence microscopy. Photo-responsive release of CSD in the PCC liposomes was determined by laser (0.5 mW/cm2) at 665 nm. Antibacterial activity of the PCC liposomes with or without irradiation was analyzed by MIC50, MBC, MBIC50, and bacterial morphology to evaluate the antibacterial effects on amoxicillin resistant Escherichia coli and Staphylococcus aureus.ResultsSize distribution, zeta potential, encapsulation efficiency and drug loading of the PCC liposomes were 189.23 ± 2.12 nm, 18.80 ± 1.57 mV, 83.52 ± 1.53% and 2.83 ± 0.05%, respectively. The PCC liposomes had higher storage stability and gastrointestinal stability, and no obvious hemolytic toxicity to rabbit red blood cells and no cytotoxicity after incubation with Hela cells. The photosensitizer pheophorbide-a was uniformly dispersed in the phospholipid layer of the PCC liposomes and increased the CSD release after irradiation. The PCC liposomes could bind to bacteria and impaired their morphology and structure, and had significant bactericidal effect on amoxicillin resistant E. coli and S. aureus.ConclusionThe photo-responsive PCC liposomes are efficient antibacterial agents for avoidance of bacterial resistance against antibiotics.

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Section: Methodsmentioning

confidence: 99%

Preparation And Antibacterial Effects Of Carboxymethyl Chitosan-Modified Photo-Responsive Camellia Sapogenin Derivative Cationic Liposomes

Zhang¹,

Ye²,

Liu³

et al. 2019

IJN

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“…There was a remarked reduction in the bacterial strain across BBB. The bacterial growth was inhibited in the infected brain of the rats by the nanocarriers that also showed low hemolysis [ 47 ]. Another novel nanocarrier made of a con-covalent complex of methoxy poly (ethylene glycol)-poly(lactide)-poly(β-amino ester) (MPEG-PLA-PAE) was designed and loaded with amphotericin-B.…”

Section: Nanomaterials: Applications In Meningitis and Bacterial Imentioning

confidence: 99%

Progress in the Application of Nanoparticles and Graphene as Drug Carriers and on the Diagnosis of Brain Infections

et al. 2021

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The blood–brain barrier (BBB) is the protective sheath around the brain that protects the sensitive microenvironments of the brain. However, certain pathogens, viruses, and bacteria disrupt the endothelial barrier and cause infection and hence inflammation in meninges. Macromolecular therapeutics are unable to cross the tight junctions, thereby limiting their bioavailability in the brain. Recently, nanotechnology has brought a revolution in the field of drug delivery in brain infections. The nanostructures have high targeting accuracy and specificity to the receptors in the case of active targeting, which have made them the ideal cargoes to permeate across the BBB. In addition, nanomaterials with biomimetic functions have been introduced to efficiently cross the BBB to be engulfed by the pathogens. This review focuses on the nanotechnology-based drug delivery approaches for exploration in brain infections, including meningitis. Viruses, bacteria, fungi, or, rarely, protozoa or parasites may be the cause of brain infections. Moreover, inflammation of the meninges, called meningitis, is presently diagnosed using laboratory and imaging tests. Despite attempts to improve diagnostic instruments for brain infections and meningitis, due to its complicated and multidimensional nature and lack of successful diagnosis, meningitis appears almost untreatable. Potential for overcoming the difficulties and limitations related to conventional diagnostics has been shown by nanoparticles (NPs). Nanomedicine now offers new methods and perspectives to improve our knowledge of meningitis and can potentially give meningitis patients new hope. Here, we review traditional diagnosis tools and key nanoparticles (Au-NPs, graphene, carbon nanotubes (CNTs), QDs, etc.) for early diagnosis of brain infections and meningitis.

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“…This is a crucial factor to keep in mind when designing new self-assembled AMPs; in fact, the antimicrobial activity may be affected because the charge distribution and the secondary structure will be different from that of single peptide molecules. Nonetheless, in some cases, the antibacterial efficacy can be considerably enhanced when peptides are self-assembled into nanostructures [55], and their stability can be enhanced because they become less sensitive towards enzymatic degradation, renal filtration, and uptake by the reticuloendothelial system [56].…”

Section: Self-assembling In Naturementioning

confidence: 99%

A New Hope: Self-Assembling Peptides with Antimicrobial Activity

et al. 2019

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Peptide drugs hold great promise for the treatment of infectious diseases thanks to their novel mechanisms of action, low toxicity, high specificity, and ease of synthesis and modification. Naturally developing self-assembly in nature has inspired remarkable interest in self-assembly of peptides to functional nanomaterials. As a matter of fact, their structural, mechanical, and functional advantages, plus their high bio-compatibility and bio-degradability make them excellent candidates for facilitating biomedical applications. This review focuses on the self-assembly of peptides for the fabrication of antibacterial nanomaterials holding great interest for substituting antibiotics, with emphasis on strategies to achieve nano-architectures of self-assembly. The antibacterial activities achieved by these nanomaterials are also described.

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TAT-modified self-assembled cationic peptide nanoparticles as an efficient antibacterial agent

Cited by 55 publications

References 32 publications

<p>Preparation And Antibacterial Effects Of Carboxymethyl Chitosan-Modified Photo-Responsive <em>Camellia</em> Sapogenin Derivative Cationic Liposomes</p>

<p>Preparation And Antibacterial Effects Of Carboxymethyl Chitosan-Modified Photo-Responsive <em>Camellia</em> Sapogenin Derivative Cationic Liposomes</p>

Progress in the Application of Nanoparticles and Graphene as Drug Carriers and on the Diagnosis of Brain Infections

A New Hope: Self-Assembling Peptides with Antimicrobial Activity

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