Tat-biliverdin reductase A inhibits inflammatory response by regulation of MAPK and NF-κB pathways in Raw 264.7 cells and edema mouse model

Tat-PRAS40 prevent hippocampal HT-22 cell death and oxidative stress induced animal brain ischemic insults

“…In a previous study, we demonstrated that PTD-antioxidant protein successfully transduced into astrocytes and significantly protected against oxidative stress-induced cell death in vitro and in vivo via its antioxidant role [19][20][21]. Moreover, we confirmed that several types of PTD fusion proteins have protective roles against cellular death in vivo and in vitro [22][23][24][25][26][27][28]. In this study, we demonstrate that Tat-PRAS40 protein significantly protects against oxidative stress-induced cellular death, suggesting that Tat-PRAS40 protein may be used as a therapeutic treatment for ischemia.…”

Effects of low doses of Tat-PIM2 protein against hippocampal neuronal cell survival

“…Tat PTD has been extensively studied and demonstrated the ability to transduce into various cells and in various animal models [20][21][22][23]. We also demonstrated the protective effects of PTDs fusion protein against cell death in vitro and in vivo [24][25][26][27][28].…”

Tat‐biliverdin reductase A protects INS‐1 cells from human islet amyloid polypeptide‐induced cytotoxicity by alleviating oxidative stress and ER stress

“…Tat-BLVRA was overexpressed in a Tat-BLVRA expression vector system based on the vector pET-15b and purified as described previously (Kim et al, 2015). Control BLVRA without Tat domain was also overexpressed and purified.…”

“…Although the mechanism remains unclear, large-sized proteins fused with protein transduction domain (PTD), such as Tat, VP22, and PEP-1, possess the ability to traverse the lipid bilayer membrane of mammalian cells (Wadia and Dowdy, 2002). Recently, we developed another cell permeable fusion protein, Tat-BLVRA, and showed that transduced Tat-BLVRA plays anti-inflammatory and anti-apoptotic roles by regulating MAPK and NFkB pathways in lipopolysaccharide-treated macrophage cells (Kim et al, 2015). Recently, we developed another cell permeable fusion protein, Tat-BLVRA, and showed that transduced Tat-BLVRA plays anti-inflammatory and anti-apoptotic roles by regulating MAPK and NFkB pathways in lipopolysaccharide-treated macrophage cells (Kim et al, 2015).…”