Taste coding of heavy metal ion-induced avoidance in Drosophila

Li, Xiaonan; Sun, Yuanjie; Gao, Shan; Li, Yan; Liu, Li; Zhu, Yan

doi:10.1016/j.isci.2023.106607

Cited by 10 publications

(6 citation statements)

References 68 publications

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“…We attempted to induce salt activation in the I-type sensilla by ectopically expressing Ir60b , under control of the Gr33a-GAL4 . Gr33a is co-expressed with Gr66a 52 , which has been shown to be co-expressed with Ir25a and Ir76b 19,53 . When we performed tip recordings from I5 and I9 sensilla, we did not observe a significant increase in action potentials in response to 300 mM NaCl ( Figure 4—figure supplement 1A ), indicating that ectopic expression of Ir60b in combination with Ir25a and Ir76b is not sufficient to generate a high salt receptor.…”

Section: Resultsmentioning

confidence: 97%

“…This indicates that IR25a, IR60b and IR76b are insufficient for sensing high Na + . The inability to confer a salt response by ectopic expression of Ir60b was not due to absence of Ir25a and Ir76b in Gr33a GRNs since Gr33a and Gr66a are co-expressed 52 , and Gr66a GRNs express Ir25a and Ir76b 19,53 . Thus, the high salt receptor in Ir60b GRNs appears to require an additional subunit.…”

Section: Discussionmentioning

confidence: 96%

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A single pair of pharyngeal neurons functions as a commander to reject high salt inDrosophila melanogaster

Sang,

Dhakal,

Shrestha

et al. 2023

Preprint

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Salt is a crucial for survival, while excessive NaCl can be detrimental. In the fruit fly, Drosophila melanogaster, an internal taste organ, the pharynx, is a critical gatekeeper impacting the decision to accept or reject a food. Currently, our understanding of the mechanism through which pharyngeal gustatory receptor neurons (GRNs) sense high salt are rudimentary. Here, we found that a member of the ionotropic receptor family, IR60b, is exclusively expressed in a pair of GRNs activated by high salt. Using a two-way choice assay (DrosoX) to measure ingestion, we demonstrate that IR60b and two coreceptors IR25a and IR76b, are required to prevent high salt consumption. Mutants lacking external taste organs but retaining the pharynx exhibit much higher salt avoidance than flies with all taste organs but missing the three IRs. Our findings highlight the critical role for IRs in a pair of pharyngeal GRNs to control ingestion of high salt.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 96%

A single pair of pharyngeal neurons functions as a commander to reject high salt inDrosophila melanogaster

Sang,

Dhakal,

Shrestha

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…We attempted to induce salt activation in the I-type sensilla by ectopically expressing Ir60b , under control of the Gr33a-GAL4. Gr33a is co-expressed with Gr66a ( Moon et al, 2009 ), which has been shown to be co-expressed with Ir25a and Ir76b ( Lee et al, 2018 ; Li et al, 2023 ). When we performed tip recordings from I5 and I9 sensilla, we did not observe a significant increase in action potentials in response to 300 mM NaCl ( Figure 4—figure supplement 1A ), indicating that ectopic expression of Ir60b in combination with Ir25a and Ir76b is not sufficient to generate a high salt receptor.…”

Section: Resultsmentioning

confidence: 99%

“…This indicates that IR25a, IR60b, and IR76b are insufficient for sensing high Na + . The inability to confer a salt response by ectopic expression of Ir60b was not due to absence of Ir25a and Ir76b in Gr33a GRNs since Gr33a and Gr66a are co-expressed ( Moon et al, 2009 ), and Gr66a GRNs express Ir25a and Ir76b ( Lee et al, 2018 ; Li et al, 2023 ). Thus, the high salt receptor in Ir60b GRNs appears to require an additional subunit.…”

Section: Discussionmentioning

confidence: 99%

A single pair of pharyngeal neurons functions as a commander to reject high salt in Drosophila melanogaster

Sang,

Dhakal,

Shrestha

et al. 2024

eLife

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Salt is a crucial for survival, while excessive NaCl can be detrimental. In the fruit fly, Drosophila melanogaster , an internal taste organ, the pharynx, is a critical gatekeeper impacting the decision to accept or reject a food. Currently, our understanding of the mechanism through which pharyngeal gustatory receptor neurons (GRNs) sense high salt are rudimentary. Here, we found that a member of the ionotropic receptor family, IR60b, is exclusively expressed in a pair of GRNs activated by high salt. Using a two-way choice assay (DrosoX) to measure ingestion, we demonstrate that IR60b and two coreceptors IR25a and IR76b, are required to prevent high salt consumption. Mutants lacking external taste organs but retaining the pharynx exhibit much higher salt avoidance than flies with all taste organs but missing the three IRs. Our findings highlight the critical role for IRs in a pair of pharyngeal GRNs to control ingestion of high salt.

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“…Interestingly, cellular responses to iron involve both receptor types or solely IRs depending if it is in the Fe 2+ or Fe 3+ form, respectively ( 61 ). Cadmium sensitivity requires an IR complex in two types of GRNS: co-receptors (IR25a, IR76b) plus IR7a in “bitter” GRNs and the same co-receptors plus IR47a in “salty” GRNs ( 63 ). A recent brief report found an additional “tuning” receptor, IR56b, to be necessary for zinc avoidance ( 62 ), however, this receptor complex detects NaCl in “sweet” GRNs for attraction ( 83 ), so this is an unexpected result.…”

Section: The Fruit Fly Taste Systemmentioning

confidence: 99%

Tastant-receptor interactions: insights from the fruit fly

Arntsen,

Guillemin,

Audette

et al. 2024

Front. Nutr.

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Across species, taste provides important chemical information about potential food sources and the surrounding environment. As details about the chemicals and receptors responsible for gustation are discovered, a complex view of the taste system is emerging with significant contributions from research using the fruit fly, Drosophila melanogaster, as a model organism. In this brief review, we summarize recent advances in Drosophila gustation and their relevance to taste research more broadly. Our goal is to highlight the molecular mechanisms underlying the first step of gustatory circuits: ligand-receptor interactions in primary taste cells. After an introduction to the Drosophila taste system and how it encodes the canonical taste modalities sweet, bitter, and salty, we describe recent insights into the complex nature of carboxylic acid and amino acid detection in the context of sour and umami taste, respectively. Our analysis extends to non-canonical taste modalities including metals, fatty acids, and bacterial components, and highlights unexpected receptors and signaling pathways that have recently been identified in Drosophila taste cells. Comparing the intricate molecular and cellular underpinnings of how ligands are detected in vivo in fruit flies reveals both specific and promiscuous receptor selectivity for taste encoding. Throughout this review, we compare and contextualize these Drosophila findings with mammalian research to not only emphasize the conservation of these chemosensory systems, but to demonstrate the power of this model organism in elucidating the neurobiology of taste and feeding.

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Taste coding of heavy metal ion-induced avoidance in Drosophila

Cited by 10 publications

References 68 publications

A single pair of pharyngeal neurons functions as a commander to reject high salt inDrosophila melanogaster

A single pair of pharyngeal neurons functions as a commander to reject high salt inDrosophila melanogaster

A single pair of pharyngeal neurons functions as a commander to reject high salt in Drosophila melanogaster

Tastant-receptor interactions: insights from the fruit fly

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