Tartrate-resistant acid phosphatase 5b (TRAP 5b) as a marker of osteoclast activity in the early phase after cementless total hip replacement

Habermann, B.; Eberhardt, Christian; Feld, Matthias; Zichner, Ludwig; Kurth, Andreas

doi:10.1080/17453670710013717

Cited by 40 publications

(25 citation statements)

References 14 publications

(17 reference statements)

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“…Here we show that TRACP and cathepsin K expression are upregulated in prelamin A-accumulating cells. Although these factors are known to mediate bone resorption [Avnet et al, 2006;Habermann et al, 2007], osteoclasts obtained in our experimental model failed to show efficient resorptive activity. This finding implies that prelamin A triggers other pathways or alters the timing of gene expression thus leading to formation of osteoclasts with reduced resorption activity.…”

Section: Discussioncontrasting

confidence: 56%

Effects of prelamin A processing inhibitors on the differentiation and activity of human osteoclasts

Zini¹,

Avnet²,

Ghisu

et al. 2008

J of Cellular Biochemistry

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Osteoclast differentiation is a complex process involving cytoskeleton and nuclear reorganization. Osteoclasts regulate bone homeostasis and have a key role in bone degenerative processes. Osteolysis and osteoporosis characterize a subset of laminopathies, inherited disorders due to defects in lamin A/C. Laminopathies featuring bone resorption are characterized, at the molecular level, by anomalous accumulation of the unprocessed lamin A precursor, called prelamin A. To obtain a suitable cell model to study prelamin A effects on osteoclasts, prelamin A processing inhibitors FTI-277 or AFCMe were applied to peripheral blood monocytes induced to differentiate towards the osteoclastic lineage. Previous studies have shown that treatment with FTI-277 causes accumulation of non-farnesylated prelamin A, while AFCMe inhibition of prelamin A maturation causes accumulation of a farnesylated form. We demonstrate that monocytes subjected to FTI-277 treatment and mostly those subjected to AFCMe administration, differentiate towards the osteoclastic lineage more efficiently than untreated monocytes, in terms of number of multinucleated giant cells, mRNA expression of osteoclast-related genes and TRACP 5b activity. On the other hand, the bone resorption activity of osteoclasts obtained in the presence of high prelamin A levels is lower with respect to control osteoclasts. This finding may help the understanding of the osteolytic and osteoporotic processes that characterize progeroid laminopathies.

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Section: Discussioncontrasting

confidence: 56%

Effects of prelamin A processing inhibitors on the differentiation and activity of human osteoclasts

Zini¹,

Avnet²,

Ghisu

et al. 2008

J of Cellular Biochemistry

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show abstract

“…TRAP is synthesized by different cells from the monohistiocytic lineage. It is also found in placenta, macrophages and leukocytes, but it is most significant expression occurs in osteoclasts, what renders this enzyme a marker of bone resorption (Miyazaki et al 2003;Fukushima et al 1991;Habermann et al 2007). In the interior of the cells, TRAP is found mainly in lysosomes, and, secondarily, in the cytoplasm or in the extracellular matrix.…”

Section: Introductionmentioning

confidence: 97%

Tartrate-resistant acid phosphatase activity and glutathione levels are modulated during hFOB 1.19 osteoblastic differentiation

et al. 2008

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Tartrate-resistant acid phosphatase (TRAP) is a well-known marker of osteoclasts and bone resorption. Here we have investigated whether osteoblast-like cells (hFOB 1.19) present TRAP activity and how would be its pattern of expression during osteoblastic differentiation. We also observed how the osteoblastic differentiation affected the reduced glutathione levels. TRAP activity was measured using the p-nitrophenylphosphate substrate. The osteogenic potential of hFOB 1.19 cells was studied by measuring alkaline phosphatase activity and mineralized nodule formation. Oxidative stress was determined by HPLC and DNTB assays. TRAP activity and the reduced glutathione-dependent microenvironment were modulated during osteoblastic differentiation. During this phase, TRAP activity, as well as alkaline phosphatase and glutathione increased progressively up to the 21st day, decreasing thereafter. We demonstrate that TRAP activity is modulated during osteoblastic differentiation, possibly in response to the redox state of the cell, since it seemed to depend on suitable levels of reduced glutathione.

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“…These markers include osteocalcin, an osteoblast-specific bone formation marker, and TRAP, an osteoclast-specific bone resorption marker (Habermann et al, 2007;Tang et al, 2011). Our study found that RYR extract decreased the bone turnover rate by suppressing osteoclast activity in OVX rats.…”

Section: Discussionmentioning

confidence: 52%

Anti-osteoporosis activity of red yeast rice extract on ovariectomy-induced bone loss in rats

et al. 2015

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ABSTRACT. Osteoporosis is the most common bone disease, affecting millions of people worldwide and leading to significant morbidity and high costs. Monacolin K, an extract of red yeast rice (RYR, Hongqu), plays important roles in the management of dyslipidemia, coronary heart disease, and diabetes. Our study aimed to investigate the protective effect of monacolin K on ovariectomy-induced bone loss in rats. Fifty female Sprague-Dawley rats were randomly divided into a sham-operated and five ovariectomized (OVX) groups: OVX with vehicle, OVX with fluvastatin, and OVX with RYR extract of three graded doses. Bone mineral density (BMD), biochemical markers, and cell viability were analyzed by dual Y.F. Wang et al. 8138©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8137-8146 (2015) energy X-ray absorptiometry, enzyme-linked immunosorbent assay, and 3(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays. Gene expression was evaluated by real-time polymerase chain reaction amplification and western blot. Our results showed that administration of RYR extract markedly increased the bone mineral density in OVX rats. Moreover, RYR extract decreased the levels of bone turnover markers, including osteocalcin and tartrate resistant acid phosphatase activity. The MMT assay revealed that RYR extract treatment significantly improved the osteoblast viabilities in a dose-dependent manner (P < 0.05). At the molecular level, we further demonstrated that RYR extract enhanced the expression of Bmp2 and Bmp4 both at the mRNA and protein levels. Collectively, these data suggested RYR extract could protect against osteoporosis in ovariectomized rats, most likely through activation of BMP2/4 expression.

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Tartrate-resistant acid phosphatase 5b (TRAP 5b) as a marker of osteoclast activity in the early phase after cementless total hip replacement

Cited by 40 publications

References 14 publications

Effects of prelamin A processing inhibitors on the differentiation and activity of human osteoclasts

Effects of prelamin A processing inhibitors on the differentiation and activity of human osteoclasts

Tartrate-resistant acid phosphatase activity and glutathione levels are modulated during hFOB 1.19 osteoblastic differentiation

Anti-osteoporosis activity of red yeast rice extract on ovariectomy-induced bone loss in rats

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