“…Trypanothione or N 1 ,N 8 ‐bis(glutathionyl)spermidine is a unique low MW molecule characteristic of trypanosomatids that replaces glutathione (GSH) as the main electron donor in the cell. It is made up of two molecules of GSH linked by a spermidine bridge (Battista, Colotti, Ilari, & Fiorillo, 2020). Trypanothione is necessary for many highly relevant cellular processes such as (i) protection of DNA from the damage generated by radicals (Awad, Henderson, Cerami, & Held, 1992); (ii) elimination of hydrogen peroxide, being much more efficient than other thiols present in trypanosomatids such as GSH, mono‐glutathionylspermidine and ovothiol A (Ariyanayagam & Fairlamb, 2001); (iii) detoxification of peroxynitrite (Thomson, Denicola, & Radi, 2003); (iv) reduction of protein and non‐protein disulfides (Ariyanayagam & Fairlamb, 2001); (v) synthesis of deoxyribonucleotides by ribonucleotide reductase (Dormeyer, Reckenfelderbäumer, Ludemann, & Krauth‐Siegel, 2001); (vi) resistance to chemotherapeutic agents (Baiocco, Colotti, Franceschini, & Ilari, 2009) and (vii) detoxification of methylglyoxal which, in almost all organisms, is detoxified by a system of GSH‐dependent glyoxalases I and II (Ariza et al, 2006; Irsch & Krauth‐Siegel, 2004).…”