Targeting the Production of Biomolecules by Extremophiles at Bioreactor Scale

Abstract

Recent alternatives to technological applications most commonly employed when the production of extremocompounds is approached at bioreactor scale, in batch, fed‐batch, and continuous mode, are summarized. Each operational mode is analyzed in the light of the more decisive variables affecting the performance of this kind of biological processes, namely, bioreactor configurations, culture medium design, operating conditions, or downstream processing. Bringing together genetic and bioreaction engineering with separation techniques is already the recommended strategy to solve the typical problems faced in bioprocesses with extremophiles and will serve to open up new opportunities for the development of unexplored fields such as renewable energies.

Aflatoxin B 1 degradation by liquid cultures and lysates of three bacterial strains

“…Shapira and Paster, 2004;Shekhar et al, 2014). Microorganisms thriving under such conditions are of great interest as their naturally developed resistance to harsh conditions makes them suitable for a wide array of biocatalytic reactions (Deive et al, 2012). Furthermore, we have earlier confirmed the potential of such microorganisms to degrade AFB 1 (Adebo et al, 2016).…”

Ionic liquids for the concomitant use in extremophiles lysis and extremozymes extraction

“…These drawbacks affect to different biotechnological processes. More specifically, extremozymes (enzymes produced by extremophiles) have been the subject of a great interest, as their naturally developed resistance to harsh reaction conditions (chemical agents and extreme values of temperature, pH and salinity) makes them suitable for an array of biocatalytic reactions (Deive et al, 2012a In our group, we have bet in thermophiles, because working at elevated temperatures involves advantages such as reduced viscosities, higher reaction rates, lower resistance to mass transfer, lower risk of contamination and higher solubility of most of the substrates and products (Rothschild and Macinelli, 2001). In this sense lipases (EC 3.1.1.3) have been recognized among the topthree most interesting group of enzymes in terms of sales, with widespread use in industrial sectors as varied as food, drugs, biofuels and detergents, just to mention a few.…”

Soft Sensor-Based Monitoring and Efficient Control Strategies of Biomass Concentration for Continuous Cultures of Haloferax mediterranei and Their Application to an Industrial Production Chain

“…Halophilic microorganisms have huge potential in terms of waste water treatment since they can be cultivated under non-sterile conditions and are able to use a broad variety of carbon sources [8][9][10]. Furthermore, they are also able to degrade a large variety of contaminants in process waters, e.g., aromatic compounds like phenol or aniline [11,12].…”