Targeting ROS/NF-κB signaling pathway by the seedless black Vitis vinifera polyphenols in CCl4-intoxicated kidney, lung, brain, and spleen in rats

Abstract

Carbon tetrachloride (CCl4) is an abundant environmental pollutant that can generate free radicals and induce oxidative stress in different human and animal organs like the kidney, lung, brain, and spleen, causing toxicity. The present study evaluated the alleviative mechanism of the isolated polyphenolic fraction from seedless (pulp and skin) black Vitis vinifera (VVPF) on systemic oxidative and necroinflammatory stress in CCl4-intoxicated rats. Here, we found that the administration of VVPF to CCl4-intoxicated rats for ten days was obviously ameliorated the CCl4-induced systemic elevation in ROS, NO and TBARS levels, as well as MPO activity. Also, it upregulated the cellular activities of the enzymatic (SOD, and GPx) and non-enzymatic (TAC and GSH) antioxidants. Furthermore, the gene expression of the ROS-related necroinflammatory mediators (NF-κB, iNOS, COX-2, and TNF-α) in the kidney, brain, and spleen, as well as IL-1β, and IL-8 in the lung were greatly restored. The histopathological studies confirmed these biochemical results and showed a noticeable enhancing effect in the architecture of the studied organs after VVPF intake. Thus, this study indicated that VVPF had an alleviative effect on CCl4-induced necroinflammation and oxidative stress in rat kidney, lung, brain, and spleen via controlling the ROS/NF-κB pathway.

N-Acetyl cysteine alleviates carbon-tetrachloride induced acute liver injury in rats

“…Further lipid peroxidation was raised because of covalent binding of CCL4 metabolites, CCL3• and CCL3OO•, to the protein in the liver cell causing destructive effects (Abbasi et al, 2021). In addition, hepatic GSH content was depleted after CCL4 administration which might be due to the extreme need of GSH to eradicate the CCL3OO• radicals and hypochlorous acid, where GSH is the key mediator in detoxification process inside the body (Habashy et al, 2021). This result corroborates with the reports of (Emmanuel et al, 2021).…”

Antifibrotic and Antioxidant Effects of a Tetrahedral Framework Nucleic Acid-Based Chlorogenic Acid Delivery System

“…In addition to Ccl4’s significant hepatotoxicity, some damage was recorded in other organs, − with the mechanism virtually linked to its increase in lipid peroxidation and free radicals. Similar phenomena were observed in this investigation.…”

Hesperidin - loaded PVA/alginate hydrogel: targeting NFκB/iNOS/COX-2/TNF-α inflammatory signaling pathway

“…Oxidative stress can impede wound healing ( 19 , 47 ). The results of extensive studies showed that pretreatment of keratinocytes (mouse skin) with H significantly reduced radiation-induced oxidative stress ( 48 ), lipid peroxidation, while increasing the expression levels of antioxidant enzymes ( 20 , 49 ).…”