Targeting Polymeric Nanobiomaterials as a Platform for Cartilage Tissue Engineering

García-Couce, Jomarien; Almirall, Amisel; Fuentes, Gastón; Kaijzel, Eric L.; Chan, Alan; Cruz, Luis J.

doi:10.2174/1381612825666190708184745

Cited by 9 publications

(4 citation statements)

References 168 publications

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“…Injectable in situ gelling systems that are based on biodegradable polymers represent promising drug delivery platforms for coping with these hurdles. Owing to their promising effects on sustained drug release, various types of polymeric hydrogels have gained considerable interest for the optimization of drug delivery [ 5 , 6 , 7 , 8 , 9 ]. Poloxamer 407 (P407), trade name Pluronic 127 ® (PF127), is a co-polymer that is based on poly (ethylene oxide)–poly (propylene oxide)–poly (ethylene oxide) triblock copolymers (EO-PO-EO) and it has found interesting applications within hydrogel drug delivery research.…”

Section: Introductionmentioning

confidence: 99%

Doxorubicin Loaded Poloxamer Thermosensitive Hydrogels: Chemical, Pharmacological and Biological Evaluation

et al. 2020

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(1) Background: doxorubicin is a potent chemotherapeutic agent, but it has limitations regarding its side effects and therapy resistance. Hydrogels potentially deal with these problems, but several characterizations need to be optimized to better understand how hydrogel assisted chemotherapy works. Poloxamer 407 (P407) hydrogels were mixed with doxorubicin and physico-chemical, biological, and pharmacological characterizations were considered. (2) Methods: hydrogels were prepared by mixing P407 in PBS at 4 °C. Doxorubicin was added upon solutions became clear. Time-to-gelation, hydrogel morphology, and micelles were studied first. The effects of P407-doxorubicin were evaluated on MC-38 colon cancer cells. Furthermore, doxorubicin release was assessed and contrasted with non-invasive in vivo whole body fluorescence imaging. (3) Results: 25% P407 had favorable gelation properties with pore sizes of 30–180 µm. P407 micelles were approximately 5 nm in size. Doxorubicin was fully released in vitro from 25% P407 hydrogel within 120 h. Furthermore, P407 micelles strongly enhanced the anti-neoplastic effects of doxorubicin on MC-38 cells. In vivo fluorescence imaging revealed that hydrogels retained fluorescence signals at the injection site for 168 h. (4) Conclusions: non-invasive imaging showed how P407 gels retained drug at the injection site. Doxorubicin P407 micelles strongly enhanced the anti-tumor effects.

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Section: Introductionmentioning

confidence: 99%

Doxorubicin Loaded Poloxamer Thermosensitive Hydrogels: Chemical, Pharmacological and Biological Evaluation

et al. 2020

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“…Specifically, the addition of 35 or fewer GAH peptides to the nanoparticles did not significantly alter final nanoparticle diameter either below or above the LCST, while conjugation levels above 41 peptides did ( Figure 3 A,B and Supplemental Tables S1 and S2 ). This insight is critical since nanoparticles with a diameter over 200 nm are known to initiate an inflammatory response in vivo [ 40 ]. A PDI of less than 0.2 signifies monodisperse particles in solution [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Hyaluronic Acid-Binding, Anionic, Nanoparticles Inhibit ECM Degradation and Restore Compressive Stiffness in Aggrecan-Depleted Articular Cartilage Explants

et al. 2021

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Joint trauma results in the production of inflammatory cytokines that stimulate the secretion of catabolic enzymes, which degrade articular cartilage. Molecular fragments of the degraded articular cartilage further stimulate inflammatory cytokine production, with this process eventually resulting in post-traumatic osteoarthritis (PTOA). The loss of matrix component aggrecan occurs early in the progression of PTOA and results in the loss of compressive stiffness in articular cartilage. Aggrecan is highly sulfated, associates with hyaluronic acid (HA), and supports the compressive stiffness in cartilage. Presented here, we conjugated the HA-binding peptide GAHWQFNALTVRGSG (GAH) to anionic nanoparticles (hNPs). Nanoparticles conjugated with roughly 19 GAH peptides, termed 19 GAH-hNP, bound to HA in solution and increased the dynamic viscosity by 94.1% compared to an HA solution treated with unconjugated hNPs. Moreover, treating aggrecan-depleted (AD) cartilage explants with 0.10 mg of 19 GAH-hNP restored the cartilage compressive stiffness to healthy levels six days after a single nanoparticle treatment. Treatment of AD cartilage with 0.10 mg of 19 GAH-hNP inhibited the degradation of articular cartilage. Treated AD cartilage had 409% more collagen type II and 598% more GAG content than untreated-AD explants. The 19 GAH-hNP therapeutic slowed ECM degradation in AD cartilage explants, restored the compressive stiffness of damaged cartilage, and showed promise as a localized treatment for PTOA.

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“…Therefore, an ideal biomaterial scaffold integrated has to provide mechanical support to an injured site assisting tissue growth without causing an inflammatory response (94). So, nanotechnology has been providing excellent products and techniques in tissue engineering and regenerative medicine and there are still many types of research in the study, mainly involving biopolymeric matrices due to its similarities with native tissues, good biological performance, and specific degradation rates (95)(96)(97)(98).…”

Section: Chemically Inherent Low Degradabilitymentioning

confidence: 99%

The impact of nanotechnology in health: A review of recent studies and products.

Menezes de Souza,

Silva,

Fabrino

et al. 2023

Rev. Cont. Saúde

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Several people suffer from diseases and injuries and many of them die because of late diagnostic and/or inefficient treatment. Many of these diseases affect humans and animals, and animals can be vectors for humans. In this perspective, it is necessary works that integrate humans, animals, and ecosystems, thus, to arise the One Health. Along with it, nanotechnology is a great alternative to improve health care, due to its revolutionary and complex properties. Although, how can nanomaterials be capable of work with or against cells, tissues, drugs, and medical devices? There are already many products in the market, but there are a lot of challenges to face and a whole new world to discover. In this article are given the application of nanostructures in diagnostic, therapy, medical devices, tissue and implant engineering, human health and animal, and a future perspective.

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Targeting Polymeric Nanobiomaterials as a Platform for Cartilage Tissue Engineering

Cited by 9 publications

References 168 publications

Doxorubicin Loaded Poloxamer Thermosensitive Hydrogels: Chemical, Pharmacological and Biological Evaluation

Doxorubicin Loaded Poloxamer Thermosensitive Hydrogels: Chemical, Pharmacological and Biological Evaluation

Hyaluronic Acid-Binding, Anionic, Nanoparticles Inhibit ECM Degradation and Restore Compressive Stiffness in Aggrecan-Depleted Articular Cartilage Explants

The impact of nanotechnology in health: A review of recent studies and products.

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