Targeting oncogenic WIP1 phosphatase sensitizes hypoxic breast cancer cells to doxorubicin induced apoptosis via activation of p53-p21 axis

Pilevneli, Hatice; Kilic-Eren, Mehtap

doi:10.1016/j.genrep.2021.101144

Cited by 2 publications

(2 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It creates a triple complex with DNA and topoisomerase II enzyme, causing it to break into DNA chains, triggering an anti-cancer response. It is known from previous studies conducted by us and others that MCF-7 cells are resistant to Etoposide (Pilevneli and Kilic Eren, 2021;Alpsoy et al, 2014). Thus, in this study similar results were also obtained.…”

Section: Discussionsupporting

confidence: 90%

“…Then, the ethanol was removed by the centrifugation and the pellet was washed with 1 ml of 1X PBS again. In the last step, PBS was removed and 200 µl of Muse Cell Cycle reagent (Brdu/PI) was added to the samples and incubated for 30 minutes at room temperature in the dark, and the results were measured with Muse Cell Analyzer (Millipore, Austin, TX, USA) (Eren et al, 2021) as described in (Pilevneli and Kilic Eren, 2021).…”

Section: Cell Cycle Analysismentioning

confidence: 99%

See 1 more Smart Citation

Investigation of the Anti-Cancer Effects of B-asaron and Etoposide in MCF-7 Breast Cancer Cells

Ak¹,

Yılmaz²,

Kaygusuz³

et al. 2022

IJIASR

View full text Add to dashboard Cite

Currently, the options available for the treatment of various cancers including breast cancer, are associated with several limitations such as severe toxicity, drug resistance, poor prognosis, and high risk of recurrence. Therefore, there appears to be an increasing interest and necessity in investigating various phytochemicals from natural sources for a superior and safer alternative treatment strategy. The bioactive phytochemical alpha (α) and beta (β)-asarone from Acorus calamus is a traditional medicine system that has been shown to have anti-tumor and chemo-inhibitory activities in numerous preclinical studies both in vitro and in vivo.Various experimental studies with human malignant cell lines and animal models have also confirmed the anti-tumor and anticancer activities of β-asarone. In this study, we aimed to investigate the anti-cancer effects of β-asarone alone or together with etoposide buy measuring cellular responses such as cell viablity, cell cycle arrest and apoptosis using breast cancer cell line MCF-7 cells. In order to get insight in to the mechanism, we also tested the expression of of NF-κB / p65 activity and the expression of Bcl-2 family member pro-apoptotic Bax protein together with p53 and p21 activities in response to β-asarone alone or together with etoposide treatment. As a result, it was concluded that the use of β-asarone alone in breast cancer cells is effective in reducing cell viability, but when used together with Etoposide, it does not cause a synergistic effect. Here we suggest that that in particular activation of NF-kB/p65 may be lead resistance to etoposide treatment.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Cell Cycle Analysismentioning

confidence: 99%