Targeting of plasmid DNA‐lipoplexes to cells with molecules anchored via a metal chelator lipid

Herringson, Thomas P.; Patlolla, Ram R.; Altin, Joseph G.

doi:10.1002/jgm.1394

Cited by 19 publications

(18 citation statements)

References 40 publications

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“…Binding assay was carried out to determine the peptide (six histidine-TAT peptide) to DOGS-NTA-Ni ratio as per reported method [24]. In brief, for the binding assay, adherent H460 cells were seeded at a density of 1×10 5 cells/well in a 24-well plate.…”

Section: Methodsmentioning

confidence: 99%

Translocation of cell penetrating peptide engrafted nanoparticles across skin layers

et al. 2010

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The objective of the current study was to evaluate the ability of cell penetrating peptides (CPP) to translocate the lipid payload into the skin layers. Fluorescent dye (DID-oil) encapsulated nano lipid crystal nanoparticles (FNLCN) were prepared using Compritol, Miglyol and DOGS-NTA-Ni lipids by hot melt homogenization technique. The FNLCN surface was coated with TAT peptide (FNLCNT) or control YKA peptide (FNLCNY) and in vitro rat skin permeation studies were performed using Franz diffusion cells. Observation of lateral skin sections obtained using cryotome with a confocal microscope demonstrated that skin permeation of FNLCNT was time dependent and after 24 h, fluorescence was observed upto a depth of 120 µm which was localized in the hair follicles and epidermis. In case of FNLCN and FNLCNY formulations fluorescence was mainly observed in the hair follicles. This observation was further supported by confocal Raman spectroscopy where higher fluorescence signal intensity was observed at 80 and 120 µm depth with FNLCNT treated skin and intensity of fluorescence peaks was in the ratio of 2:1:1 and 5:3:1 for FNLCNT, FNLCN, and FNLCNY treated skin sections, respectively. Furthermore, replacement of DID-oil with celecoxib (Cxb), a model lipophilic drug showed similar results and after 24 h, the CXBNT formulation increased the Cxb concentration in SC by 3 and 6 fold and in epidermis by 2 and 3 fold as compared to CXBN and CXBNY formulations respectively. Our results strongly suggest that CPP can translocate nanoparticles with their payloads into deeper skin layers.

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Section: Methodsmentioning

confidence: 99%

Translocation of cell penetrating peptide engrafted nanoparticles across skin layers

et al. 2010

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“…The fluorescent lipid Oregon Green-488-(1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine) (OG 488 -DHPE) was purchased from Invitrogen (Eugene, OR). 1,2-palmitoyl-oleoyl-snglycero-3-phosphatidylethanolamine was reacted with Alexa Fluor-647 carboxylic acid succinimidyl ester (Invitrogen) and purified to produce Alexa Fluor-647-1,2-palmitoyl-oleoyl-sn-glycero-3-phosphatidylethanolamine (AF 647 -DOPE), as previously described (30). Paraformaldehyde was from BDH Chemicals (Kilsyth, Victoria, Australia).…”

Section: Reagentsmentioning

confidence: 99%

Antigen-Containing Liposomes Engrafted with Flagellin-Related Peptides Are Effective Vaccines That Can Induce Potent Antitumor Immunity and Immunotherapeutic Effect

Faham

Altin

2010

The Journal of Immunology

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“…Other promising innovative approaches for ligating ligands to the liposome surface include transpeptidation catalyzed by sortase A [79] and the chelating strategy employing His-tagged proteins and nitrilotriacetic acid (NTA 3 ) modified liposomes [84–86]. The latter strategy has opened the opportunity to modify liposomes with a vast variety of His-tag possessing proteins (or peptides).…”

Section: Discussionmentioning

confidence: 99%

Ligation Strategies for Targeting Liposomal Nanocarriers

Marqués-Gallego

Kroon

2014

BioMed Research International

109

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Liposomes have been exploited for pharmaceutical purposes, including diagnostic imaging and drug and gene delivery. The versatility of liposomes as drug carriers has been demonstrated by a variety of clinically approved formulations. Since liposomes were first reported, research of liposomal formulations has progressed to produce improved delivery systems. One example of this progress is stealth liposomes, so called because they are equipped with a PEGylated coating of the liposome bilayer, leading to prolonged blood circulation and improved biodistribution of the liposomal carrier. A growing research area focuses on the preparation of liposomes with the ability of targeting specific tissues. Several strategies to prepare liposomes with active targeting ligands have been developed over the last decades. Herein, several strategies for the functionalization of liposomes are concisely summarized, with emphasis on recently developed technologies for the covalent conjugation of targeting ligands to liposomes.

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Targeting of plasmid DNA‐lipoplexes to cells with molecules anchored via a metal chelator lipid

Cited by 19 publications

References 40 publications

Translocation of cell penetrating peptide engrafted nanoparticles across skin layers

Translocation of cell penetrating peptide engrafted nanoparticles across skin layers

Antigen-Containing Liposomes Engrafted with Flagellin-Related Peptides Are Effective Vaccines That Can Induce Potent Antitumor Immunity and Immunotherapeutic Effect

Ligation Strategies for Targeting Liposomal Nanocarriers

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