Targeting inflammation and oxidative stress for protection against ischemic brain injury in rats using cupressuflavone

Albaqami, Faisal F.; Abdel-Rahman, Rehab F.; Althurwi, Hassan N.; Alharthy, Khalid M.; Soliman, Gamal A.; Aljarba, Tariq M.; Ogaly, Hanan A.; Abdel-Kader, Maged S.

doi:10.1016/j.jsps.2023.101933

Cited by 3 publications

(2 citation statements)

References 49 publications

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“…As per Albaqami et al (2024) , paraffin slices of testicles were adhered to positively charged slides via the avidin–biotin–peroxidase complex (ABC) technique, primary antibody. After these antibodies were incubated on sections from each group, the chemicals needed for the ABC technique were added (Vectastain ABC-HRP kit, Vector laboratories).…”

Section: Methodsmentioning

confidence: 99%

Propolis attenuates diabetes-induced testicular injury by protecting against DNA damage and suppressing cellular stress

Ashour

2024

Front. Pharmacol.

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Introduction: Propolis has a wide range of biological and pharmacological actions, including antioxidant properties—particularly its phenolic and flavonoid constituents—that could potentially protect the reproductive system from oxidative damage.Method: Four groups were allocated 40 male Wistar rats each. The vehicle was given to the first group’s normal control rats negative control. The second, third, and fourth groups of diabetic rats were given vehicle (diabetic control) and propolis orally at 50 and 100 mg/kg, respectively, for 8 weeks. Diabetes was induced in rats via injection of nicotinamide and streptozotocin (STZ). Fasting blood glucose (FBG) and insulin levels, homeostatic model assessment for insulin resistance (HOMA-IR), and semen analysis were assessed. In addition, assessments of serum reproductive hormones, including total testosterone (TTST), estradiol (E2), follicle-stimulating hormone luteinizing hormone (LH), and prolactin (PRL), were measured at the end of the study. Tissue total testosterone, E2, and dihydrotestosterone were also evaluated. Serum and tissue oxidative enzymes, including catalase (CAT), superoxide dismutase, and glutathione peroxidase activities, were examined, and malondialdehyde content was determined. The pancreatic and testicular tissues were histopathologically examined, and proliferating cell nuclear antigen (PCNA) and B-cell lymphoma 2 (Bcl-2) in testicular tissue were immunohistochemically analyzed. Testicular tissue was examined for DNA integrity using a comet assay.Results: Compared to the STZ-control group, propolis greatly decreased FBG levels and improved the glycemic status of diabetic rats. In comparison to the STZ-DC group, propolis increased the number of sperm cells and the percent of morphologically normal and viable sperm in male rats, improving their fertility. Propolis also restored the pancreatic islets, protected the testis from oxidative stress, and increased levels of reproductive hormones in the blood, especially testosterone. Moreover, propolis at high doses demonstrated a strong positive response for Bcl-2 and a negative expression of proliferating cell nuclear antigen in spermatogenic cells.Conclusion: The data obtained strongly indicate that STZ causes severe impairments to the testis whereas propolis, acting as an antioxidant, protects against the adverse effects of STZ on the testis.

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Section: Methodsmentioning

confidence: 99%

Propolis attenuates diabetes-induced testicular injury by protecting against DNA damage and suppressing cellular stress

Ashour

2024

Front. Pharmacol.

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“…A robust inflammatory response occurs after ischemic stroke (Albaqami et al., 2024 ; Fu et al., 2023 ). Ischemic brain tissues release chemokines and cytokines and recruit peripheral circulating neutrophils within hours, followed by lymphocyte (Bui et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Dynamic change of neutrophil‐to‐lymphocyte ratio and its predictive value of prognosis in acute ischemic stroke

Qian,

Hu,

Wang

et al. 2024

Brain and Behavior

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ObjectiveThe present research aimed to explore the dynamic change of the neutrophil‐to‐lymphocyte ratio (NLR) and its relationship with functional outcome following an acute ischemic stroke (AIS), whether receiving intravenous thrombolysis (IVT) or not.MethodsWe retrospectively analyzed data that were prospectively acquired from patients with AIS treated with IVT or not. For patients receiving IVT, the NLR was based on a blood test performed prior to IVT (d0) and at different time points after disease onset (d1, d3, d7). In addition, in the non‐IVT group, the NLR was obtained at different time points after disease onset (d1, d3, d7). Follow‐ups were performed 3 months after onset via telephone. In addition, a good outcome was defined as a modified Rankin scale (mRS) ≤1; a poor outcome means 2 ≤ mRS ≤ 6.ResultsA total of 204 AIS patients were included in this study. The NLR presented a dynamic change as it increased to its peak at day 1 and gradually declined to its baseline at day 7, no matter whether patients were receiving IVT or not. Patients with poor outcomes have a higher NLR at various time points. The results of multivariate logistic regression analysis demonstrated that the National Institutes of Health Stroke Scale (NIHSS), NLR d1, NLR d3, and NLR d7 were independently associated with functional outcomes. The area under the receiver operating characteristic curve of NLR in predicting outcomes was as follows: NLR d3 demonstrated robust predictive power within the IVT therapy cohort, whereas NLR d7 was predictive in the non‐IVT cohort. However, the most potent predictor emerged as the combination of NIHSS and NLR.ConclusionNLR has the potential to predicate diagnosis for AIS, especially when combined with the NIHSS score.

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Neuroprotective effects of Hibiscus sabdariffa var. altissima on cerebral ischemia‒Reperfusion injury in rats

Hamadjida,

Njemguie,

Mbomo

et al. 2024

Pharmacological Research - Modern Chinese Medicine

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Targeting inflammation and oxidative stress for protection against ischemic brain injury in rats using cupressuflavone

Cited by 3 publications

References 49 publications

Propolis attenuates diabetes-induced testicular injury by protecting against DNA damage and suppressing cellular stress

Propolis attenuates diabetes-induced testicular injury by protecting against DNA damage and suppressing cellular stress

Dynamic change of neutrophil‐to‐lymphocyte ratio and its predictive value of prognosis in acute ischemic stroke

Neuroprotective effects of Hibiscus sabdariffa var. altissima on cerebral ischemia‒Reperfusion injury in rats

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