Targeting Hydroxybenzoic Acids to Mitochondria as a Strategy to Delay Skin Ageing: An In Vitro Approach

Fernandes, Carlos; Cagide, Fernando; Simões, Jorge; Pita, Carlos; Pereira, Eurico; Videira, Afonso J. C.; Soares, Pedro; Duarte, José F. S.; Santos, António M. S.; Oliveira, Paulo J.; Borges, Fernanda; Silva, Filomena S. G.

doi:10.3390/molecules27196183

Cited by 3 publications

(3 citation statements)

References 70 publications

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“…In addition, protocatechuic acid shows a potential stimulating effect on collagen synthesis and has shown the ability to inhibit the effects of UVA radiation on the skin, resulting in reduced wrinkles [28]. A similar effect is shown by m-hydroxybenzoic acid, which reduces the effects of oxidative stress in the skin and therefore delays the skin aging process [29]. Furthermore, in addition to phenolic acids, a compound that has largely accumulated in the skin is caffeine, which is commonly used in cosmetic products.…”

Section: Discussionmentioning

confidence: 99%

Kombucha as a Potential Active Ingredient in Cosmetics—An Ex Vivo Skin Permeation Study

Jakubczyk,

Nowak,

Muzykiewicz-Szymańska

et al. 2024

Molecules

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Kombucha is a non-alcoholic beverage, that is increasingly used in the cosmetic industry. The available literature reports the positive effects of kombucha on the skin, in particular its antioxidant action. However, there is a lack of information on skin permeation and the accumulation of active ingredients showing such effects. Skin aging is largely dependent on oxidative stress, therefore in our study we assessed the ex vivo permeation of two types of kombucha (green and black tea) through porcine skin. The antioxidant activity (DPPH, ABTS, FRAP methods) and total polyphenol content of these extracts were determined before and after permeation testing. Moreover, the content of selected phenolic acids as well as caffeine was assessed. Skin permeation was determined using a Franz diffusion cell. The antioxidant activity of both Kombuchas was found to be high. In addition, gallic acid, chlorogenic acid, protocatechuic acid, coumaric acid, m-hydroxybenzoic acid, and caffeine were identified. A 24-h ex vivo study showed the permeation of some phenolic acids and caffeine and their accumulation in the skin. Our results confirm the importance of studying the skin permeation of what are still little known ingredients in cosmetic preparations. Evaluation of the accumulation of these ingredients can guarantee the efficacy of such preparations.

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Section: Discussionmentioning

confidence: 99%

Kombucha as a Potential Active Ingredient in Cosmetics—An Ex Vivo Skin Permeation Study

Jakubczyk,

Nowak,

Muzykiewicz-Szymańska

et al. 2024

Molecules

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“…AntiOxCIN4, derived from the conjugation of caffeic acid with an alkyl linker and TPP + , has been shown to improve liver steatosis in Western diet-fed mice [52], revert cellular and mitochondrial abnormalities in male sporadic Parkinson's disease patients' skin fibroblasts [244], and stimulate ROS-protective pathways in primary human skin fibroblasts by inducing Nrf2 and increasing superoxide dismutase 2 (SOD2) and GSH levels [245]. AntiOxBEN2, which is derived from hydroxybenzoic acid conjugated with TPP + , presented promising results in delaying aging by preventing senescence in skin cells [246], indicating that both AntiOxCIN4 and AntiOxBEN2 may act as pro-oxidants, triggering internal ROS defense mechanisms [245]. SkQ1, a mitochondria-targeting derivative of plastoquinone and TPP + , has been used in preclinical studies for the treatment of cardiovascular, renal, and inflammatory bowel diseases in an intestinal epithelium mice model and Caco-2 cells [247].…”

Section: Mitochondria-targeted Drugsmentioning

confidence: 99%

Metabolic Priming as a Tool in Redox and Mitochondrial Theragnostics

2023

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Theragnostics is a promising approach that integrates diagnostics and therapeutics into a single personalized strategy. To conduct effective theragnostic studies, it is essential to create an in vitro environment that accurately reflects the in vivo conditions. In this review, we discuss the importance of redox homeostasis and mitochondrial function in the context of personalized theragnostic approaches. Cells have several ways to respond to metabolic stress, including changes in protein localization, density, and degradation, which can promote cell survival. However, disruption of redox homeostasis can lead to oxidative stress and cellular damage, which are implicated in various diseases. Models of oxidative stress and mitochondrial dysfunction should be developed in metabolically conditioned cells to explore the underlying mechanisms of diseases and develop new therapies. By choosing an appropriate cellular model, adjusting cell culture conditions and validating the cellular model, it is possible to identify the most promising therapeutic options and tailor treatments to individual patients. Overall, we highlight the importance of precise and individualized approaches in theragnostics and the need to develop accurate in vitro models that reflect the in vivo conditions.

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“…However, dasatinib is not specific to skin-related anti-aging methods, and metformin, rapamycin, and fisetin have not been tested in population trials) [ 19 , 20 , 21 , 22 ]. In addition, the reasonable local application of antioxidants is an effective protection strategy against skin aging including some pharmacological drugs, antioxidants and active chemical compounds such as CoQ10 [ 23 ], ginsenoside RG3 [ 24 ], nicotinamide [ 25 ], HCP1 [ 26 ], h hydroxyproline [ 27 ], mitochondria-targeted H 2 S [ 28 ]. Nucleotides (NTs) are fundamental components of nucleic acids and play crucial roles in various intricate physiological processes such as energy metabolism, intercellular signal transduction, enzymatic reactions, and cell cycle regulation as a synthetic nitrogen source.…”

Section: Introductionmentioning

confidence: 99%

Exogenous Nucleotides Improve the Skin Aging of SAMP8 Mice by Modulating Autophagy through MAPKs and AMPK Pathways

Fan,

Zhang,

Liu

et al. 2024

Nutrients

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The skin, serving as the body’s primary defense against external elements, plays a crucial role in protecting the body from infections and injuries, as well as maintaining overall homeostasis. Skin aging, a common manifestation of the aging process, involves the gradual deterioration of its normal structure and repair mechanisms. Addressing the issue of skin aging is increasingly imperative. Multiple pieces of evidence indicate the potential anti-aging effects of exogenous nucleotides (NTs) through their ability to inhibit oxidative stress and inflammation. This study aims to investigate whether exogenous NTs can slow down skin aging and elucidate the underlying mechanisms. To achieve this objective, senescence-accelerated mouse prone-8 (SAMP8) mice were utilized and randomly allocated into Aging, NTs-low, NTs-middle, and NTs-high groups, while senescence-accelerated mouse resistant 1 (SAMR1) mice were employed as the control group. After 9 months of NT intervention, dorsal skin samples were collected to analyze the pathology and assess the presence and expression of substances related to the aging process. The findings indicated that a high-dose NT treatment led to a significant increase in the thickness of the epithelium and dermal layers, as well as Hyp content (p < 0.05). Additionally, it was observed that low-dose NT intervention resulted in improved aging, as evidenced by a significant decrease in p16 expression (p < 0.05). Importantly, the administration of high doses of NTs could improve, in some ways, mitochondrial function, which is known to reduce oxidative stress and promote ATP and NAD+ production significantly. These observed effects may be linked to NT-induced autophagy, as evidenced by the decreased expression of p62 and increased expression of LC3BI/II in the intervention groups. Furthermore, NTs were found to upregulate pAMPK and PGC-1α expression while inhibiting the phosphorylation of p38MAPK, JNK, and ERK, suggesting that autophagy may be regulated through the AMPK and MAPK pathways. Therefore, the potential induction of autophagy by NTs may offer benefits in addressing skin aging through the activation of the AMPK pathway and the inhibition of the MAPK pathway.

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Targeting Hydroxybenzoic Acids to Mitochondria as a Strategy to Delay Skin Ageing: An In Vitro Approach

Cited by 3 publications

References 70 publications

Kombucha as a Potential Active Ingredient in Cosmetics—An Ex Vivo Skin Permeation Study

Kombucha as a Potential Active Ingredient in Cosmetics—An Ex Vivo Skin Permeation Study

Metabolic Priming as a Tool in Redox and Mitochondrial Theragnostics

Exogenous Nucleotides Improve the Skin Aging of SAMP8 Mice by Modulating Autophagy through MAPKs and AMPK Pathways

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