Targeting glioblastoma stem cells: The first step of photodynamic therapy

Aguilar, Lechner Rodríguez; Vilchez, María Laura; Sanabria, Laura Natalia Milla

doi:10.1016/j.pdpdt.2021.102585

Cited by 9 publications

(5 citation statements)

References 96 publications

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“…This means that at values equal to or higher than the applied light dose, all cell samples showed different response from PDT treatment. This can be explained with the presence of a various number of glioma stem cells in each tumor, which according to the literature, is responsible for the different resistance to the used procedure [10].…”

Section: Conclusion and Discussionmentioning

confidence: 99%

PDT and PD of Human Glioblastoma with 5-ALA/PpIX and n-GalLuPc

Zaharieva

Ангелов

Genova

et al. 2023

J. Phys.: Conf. Ser.

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Glioblastoma (GBM) is the most common and severe type of brain tumor. Surgery and subsequent radiotherapy and chemotherapy do not lead to sufficient results in the treatment of this type of malignancy, mostly due to its specific morphology. Only about 6% of the patients of advanced age survive 5 years after being diagnosed with GBM. Therefore, scientists are working on alternative therapies that would lead to more effective and long-term treatment of glioblastoma. Photodynamic therapy (PDT) and photodiagnostics (PD) are such unconventional methods for treating and diagnosing malignant tumors. During our work, more than 20 experiments were carried out with stem cells cultivated from human glioblastoma tumors. We used two types of photosensitizers – delta-aminolevulinic acid (5-ALA) as a precursor of protoporphyrin IX (PpIX) and non-peripherally galactosylated lutetium phthalocyanine (n-GalLuPc). After the irradiation supernatant samples of photosensitizer-treated cell lines were used for evaluation of photosensitizers’ accumulation in the cell lines investigated. The emitting spectra is correlated with the total induced cell death in the treated cells. After considering the overall effectiveness of the two photosensitizers, n-GalLuPc showed higher efficiency. These in vitro results prompted the future investigation of the phthalocyanine for in vivo application in GBM treatments.

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Section: Conclusion and Discussionmentioning

confidence: 99%

PDT and PD of Human Glioblastoma with 5-ALA/PpIX and n-GalLuPc

Zaharieva

Ангелов

Genova

et al. 2023

J. Phys.: Conf. Ser.

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“…12 Their absorption of NIR in particular makes AuNCs ideal materials for phototherapy because of the strong tissue penetration and less tissue damage from NIR. 13 In cancer therapy, tumor targeting is generally achieved by drugs, immune factors, 14 cytokines, 15 and so on. Due to the abundant blood vessels, wide vascular wall space, poor structural integrity, and lack of lymphatic reflux in solid tumor tissues, some macromolecular substances of specific sizes, such as nanoparticles (NPs) and some macromolecular drugs, are more likely to penetrate tumor tissues and remain for a long time (compared with normal tissues).…”

Section: Introductionmentioning

confidence: 99%

A Review on Gold Nanoclusters for Cancer Phototherapy

Yang,

Guo

et al. 2023

ACS Appl. Bio Mater.

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Cancer phototherapy, including photodynamic therapy (PDT) and photothermal therapy (PTT), has been extensively studied in recent years because of its noninvasive properties, high efficiency, improved selectivity, and reduced side effects. Gold nanoclusters (AuNCs) have the advantages of high biocompatibility, high biosafety, excellent photoresponse, and high tumor penetration ability. This review analyzes the use of AuNCs in tumor phototherapy in recent years from three aspects, namely, AuNCs in PDT, AuNCs in PTT, and AuNCs in combination therapy, and presents the high potential of AuNCs in cancer phototherapy. This review aims to provide readers with the unique advantages, diversified application approaches, and bright application prospects of AuNCs in phototherapy and to provide insights into strategies for applying AuNCs to tumor phototherapy.

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“…Additionally, the 5-ALA compound is a precursor to the potent photosensitizer, protoporphyrin IX ( PpIX ). When PpIX is excited by 635 nm light, it generates triplet oxygen species triggering cytotoxicity as a photosensitizer [ 11 , 12 , 13 , 14 , 15 , 16 ]. Therefore, if we use both the fluorophore and photosensitizer (PS) capabilities of protoporphyrin IX ( PpIX ) at the same time, we will be able to visually identify neoplastic tissue and, at the same time, we can selectively destroy that tissue, thus improving the GB treatment success.…”

Section: Introductionmentioning

confidence: 99%

Preclinical Studies with Glioblastoma Brain Organoid Co-Cultures Show Efficient 5-ALA Photodynamic Therapy

Pedrosa

Bedia

Diao

et al. 2023

Cells

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Background: The high recurrence of glioblastoma (GB) that occurs adjacent to the resection cavity within two years of diagnosis urges an improvement of therapies oriented to GB local control. Photodynamic therapy (PDT) has been proposed to cleanse infiltrating tumor cells from parenchyma to ameliorate short long-term progression-free survival. We examined 5-aminolevulinic acid (5-ALA)-mediated PDT effects as therapeutical treatment and determined optimal conditions for PDT efficacy without causing phototoxic injury to the normal brain tissue. Methods: We used a platform of Glioma Initiation Cells (GICs) infiltrating cerebral organoids with two different glioblastoma cells, GIC7 and PG88. We measured GICs-5-ALA uptake and PDT/5-ALA activity in dose-response curves and the efficacy of the treatment by measuring proliferative activity and apoptosis. Results: 5-ALA (50 and 100 µg/mL) was applied, and the release of protoporphyrin IX (PpIX) fluorescence measures demonstrated that the emission of PpIX increases progressively until its stabilization at 24 h. Moreover, decreased proliferation and increased apoptosis corroborated the effect of 5-ALA/PDT on cancer cells without altering normal cells. Conclusions: We provide evidence about the effectiveness of PDT to treat high proliferative GB cells in a complex in vitro system, which combines normal and cancer cells and is a useful tool to standardize new strategic therapies.

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Targeting glioblastoma stem cells: The first step of photodynamic therapy

Cited by 9 publications

References 96 publications

PDT and PD of Human Glioblastoma with 5-ALA/PpIX and n-GalLuPc

PDT and PD of Human Glioblastoma with 5-ALA/PpIX and n-GalLuPc

A Review on Gold Nanoclusters for Cancer Phototherapy

Preclinical Studies with Glioblastoma Brain Organoid Co-Cultures Show Efficient 5-ALA Photodynamic Therapy

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