Targeting Cuticular Components for Pest Management

Doucet, Daniel; Retnakaran, Arthur

doi:10.1007/978-3-319-40740-1_10

Cited by 3 publications

(1 citation statement)

References 174 publications

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“…Both these isolates are known to produce toxic proteins that enhance their virulence; their extracts cause temporary tetanic paralysis in Galleria mellonela (L.) larvae (Ortiz-Urquiza et al ., 2010). Tetanic paralysis occurs as a result of the membrane Ca 2+ channels opening; it influences chitin synthesis which can result in deformities (Doucet and Retnakaran, 2016).…”

Section: Discussionmentioning

confidence: 99%

Soil drenching with entomopathogenic fungi for control of the soil-dwelling life stages and adults of the same generation ofSpodoptera littoralis(Boisd.) (Lepidoptera: Noctuidae)

Garrido‐Jurado

Resquín-Romero

Yousef-Yousef

et al. 2019

Bull. Entomol. Res.

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Four Beauveria bassiana and three Metarhizium brunneum isolates were evaluated, as soil drenches, against Spodoptera littoralis prepupae. Treatment efficacy was determined by assessing total mortality during development from prepupae through to pupae and adults; mortality and sub-lethal effects on reproduction were also quantified for adults emerging from surviving prepupae/pupae. All isolates were pathogenic but overall mortality varied between 31.7 and 83.3% (0% for control); average survival time was 7.5–10.5 days (14.0 days for control). From 1.7–15.0% of adults emerging from surviving prepupae/pupae were deformed (0% in control). Contact with fungal suspensions as prepupae/pupae caused a significant reduction in fecundity of emerging adult females (15–58.9%), and a significant reduction in egg viability (6.8–28.4%) compared with controls. Two isolates were selected for virulence evaluation against S. littoralis prepupae. The LC50s were 1.7 × 107 and 1.8 × 107 conidia ml−1 and the median survival times were 7 and 6 days for isolates EAMa 01/58-Su and EAMb 09/01-Su, respectively. Destruxin A was present in pupae developing from prepupae treated with isolates EAMa 01/58-Su (0.010 ± 0.002 µg pupae−1) and EAMb 09/01-Su (0.015 ± 0.003 µg pupae−1). The use of entomopathogenic fungi as soil drenches could be a key component of S. littoralis IPM strategies due to direct reductions in the number of soil-dwelling life stages and, also, the significant reduction in reproductive potential of surviving adults.

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Section: Discussionmentioning

confidence: 99%

Soil drenching with entomopathogenic fungi for control of the soil-dwelling life stages and adults of the same generation ofSpodoptera littoralis(Boisd.) (Lepidoptera: Noctuidae)

Garrido‐Jurado

Resquín-Romero

Yousef-Yousef

et al. 2019

Bull. Entomol. Res.

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Chito-Protein Matrices in Arthropod Exoskeletons and Peritrophic Matrices

Zhao

Zhang

Zhu

2019

Biologically-Inspired Systems

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RNA-seq Analysis Reveals Mitochondrial and Cuticular Protein Genes Are Associated with Phosphine Resistance in the Rusty Grain Beetle (Coleoptera:Laemophloeidae)

Chen

Duan

Song

et al. 2020

Journal of Economic Entomology

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The rusty grain beetle, Cryptolestes ferrugineus (Stephens), is a serious pest of stored grain, which has developed high levels of resistance to phosphine. In this study, five geographically distant populations of C. ferrugineus had been collected in China, specifically in granaries where phosphine fumigant is used for pest control, and they showed a high resistance ratio up to 1,907 (LC50 = 21.0 mg/liter). Then, a reference transcriptome was constructed to use as a basis for investigating the molecular mechanisms of phosphine resistance in this species, which consisted of 47,006 unigenes with a mean length of 1,090. Subsequently, the RNA-Seq analysis of individuals from the most susceptible and resistant populations led to the identification of 54 genes that are differentially expressed. GO and KEGG analysis demonstrated that genes associated with mitochondrial and respiration functions were significantly enriched. Also, the ‘structural constituent of cuticle’ term was annotated in the GO enrichment analysis and further qRT-PCR confirmed that the expression levels of nine cuticular protein genes were significantly increased in the resistant population. In conclusion, we present here a transcriptome-wide overview of gene expression changes between resistant and susceptible populations of C. ferrugineus, and this in turn documents that mitochondria and cuticular protein genes may play together a crucial role in phosphine resistance. Further gene function analysis should enable the provision of advice to expedite resistance management decisions.

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Targeting Cuticular Components for Pest Management

Cited by 3 publications

References 174 publications

Soil drenching with entomopathogenic fungi for control of the soil-dwelling life stages and adults of the same generation ofSpodoptera littoralis(Boisd.) (Lepidoptera: Noctuidae)

Soil drenching with entomopathogenic fungi for control of the soil-dwelling life stages and adults of the same generation ofSpodoptera littoralis(Boisd.) (Lepidoptera: Noctuidae)

Chito-Protein Matrices in Arthropod Exoskeletons and Peritrophic Matrices

RNA-seq Analysis Reveals Mitochondrial and Cuticular Protein Genes Are Associated with Phosphine Resistance in the Rusty Grain Beetle (Coleoptera:Laemophloeidae)

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