Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy

Kahle, Elizabeth R.; Patel, Neil; Sreenivasappa, Harini; Marcolongo, Michele; Han, Lin

doi:10.1016/j.pbiomolbio.2022.08.005

Cited by 10 publications

(7 citation statements)

References 130 publications

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“…R. Kahle et al describe the cellular biomechanics and mechanotransduction of articular cartilage through an integrated system using AFM (as a biomechanical testing tool) with complementary immunofluorescence (IF) imaging (as an in situ navigation system). These promote the basic knowledge of extracellular matrix biomechanics and cell mechanobiology, improving the understanding, detection, and intervention of various diseases (Kahle et al, 2022). Dominick J. Romano et al probed the topography and stiffness of endothelial cells using atomic force microscopy (AFM), while time‐lapse microscopy provided observations of the wound healing model.…”

Section: Discussionmentioning

confidence: 99%

“…These promote the basic knowledge of extracellular matrix biomechanics and cell mechanobiology, improving the understanding, detection, and intervention of various diseases (Kahle et al, 2022). Dominick J. Romano et al probed the topography and stiffness of endothelial cells using atomic force microscopy (AFM), while timelapse microscopy provided observations of the wound healing model.…”

Section: Mean Fluorescence Intensity Images Of Calcium In Ventricular...mentioning

confidence: 99%

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AFM is used to study the biophysics of hypertension‐induced tachyarrhythmia

Yin

Sun

et al. 2023

Microscopy Res & Technique

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Patients with long‐lasting hypertension often suffer from atrial or ventricular arrhythmias. Evidence suggests that mechanical stimulation can change the refractory period and dispersion of the ventricular myocyte action potential through stretch‐activated ion channels (SACs) and influence cellular calcium transients, thus increasing susceptibility to ventricular arrhythmias. However, the specific pathogenesis of hypertension‐induced arrhythmias is unknown. In this study, through clinical data, we found that a short‐term increase in blood pressure leads to a rise in tachyarrhythmias in patients with clinical hypertension. We investigated the mechanism of this phenomenon using a combined imaging system(AC) of atomic force microscopy (AFM) and laser scanning confocal microscopy. After mechanical distraction to stimulate ventricular myocytes isolated from Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR), we synchronously monitored cardiomyocyte stiffness and intracellular calcium changes. This method can reasonably simulate cardiomyocytes' mechanics and ion changes when blood pressure rises rapidly. Our results indicated that the stiffness value of cardiomyocytes in SHR was significantly more extensive than that of normal controls, and cardiomyocytes were more sensitive to mechanical stress; In addition, intracellular calcium increased rapidly and briefly in rats with spontaneous hypertension. After intervention with streptomycin, a SAC blocker, ventricular myocytes are significantly less sensitive to mechanical stimuli. Thus, SAC is involved in developing and maintaining ventricular arrhythmias induced by hypertension. The increased stiffness of ventricular myocytes caused by hypertension leads to hypersensitivity of cellular calcium flow to mechanical stimuli is one of the mechanisms that cause arrhythmias. The AC system is a new research method to study the mechanical properties of cardiomyocytes. This study provides new techniques and ideas for developing new anti‐arrhythmic drugs.HighlightThe mechanism of hypertension‐induced tachyarrhythmia is not precise. Through this study, it is found that the biophysical properties of myocardial abnormalities, the myocardium is excessively sensitive to mechanical stimulation, and the calcium flow appears to transient explosive changes, leading to tachyarrhythmia.

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Section: Discussionmentioning

confidence: 99%

Section: Mean Fluorescence Intensity Images Of Calcium In Ventricular...mentioning

confidence: 99%

AFM is used to study the biophysics of hypertension‐induced tachyarrhythmia

Yin

Sun

et al. 2023

Microscopy Res & Technique

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“…AFM with IF imaging has been used as a mechanical force transducer to uncover the dynamic changes in the cytoskeletal organization and nuclear architecture. 315 In live endothelial cells, the cortical actin network dynamics have been observed by AFM coupled with confocal fluorescence microscopy. 316 In cancer study, AFM-nanoindentation integrated with spinning desk confocal microscopy (SDCM) has been utilized to observe local deformation in the cell membrane and cytoskeleton in NIH 3T3 fibroblasts and MDA-MB-231 breast cancer epithelial cells.…”

Section: Atomic Force Microscopy (Afm)mentioning

confidence: 99%

“…AFM can be used synergistically with immunofluorescence (IF) imaging to study molecular mechanisms behind the mechanotransduction pathway. AFM with IF imaging has been used as a mechanical force transducer to uncover the dynamic changes in the cytoskeletal organization and nuclear architecture 315 . In live endothelial cells, the cortical actin network dynamics have been observed by AFM coupled with confocal fluorescence microscopy 316 .…”

Section: Techniques For Studying Mechano‐epigeneticsmentioning

confidence: 99%

Mechanotransduction and epigenetic modulations of chromatin: Role of mechanical signals in gene regulation

Mishra,

Chakraborty,

Niharika

et al. 2024

J of Cellular Biochemistry

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Mechanical forces may be generated within a cell due to tissue stiffness, cytoskeletal reorganization, and the changes (even subtle) in the cell's physical surroundings. These changes of forces impose a mechanical tension within the intracellular protein network (both cytosolic and nuclear). Mechanical tension could be released by a series of protein–protein interactions often facilitated by membrane lipids, lectins and sugar molecules and thus generate a type of signal to drive cellular processes, including cell differentiation, polarity, growth, adhesion, movement, and survival. Recent experimental data have accentuated the molecular mechanism of this mechanical signal transduction pathway, dubbed mechanotransduction. Mechanosensitive proteins in the cell's plasma membrane discern the physical forces and channel the information to the cell interior. Cells respond to the message by altering their cytoskeletal arrangement and directly transmitting the signal to the nucleus through the connection of the cytoskeleton and nucleoskeleton before the information despatched to the nucleus by biochemical signaling pathways. Nuclear transmission of the force leads to the activation of chromatin modifiers and modulation of the epigenetic landscape, inducing chromatin reorganization and gene expression regulation; by the time chemical messengers (transcription factors) arrive into the nucleus. While significant research has been done on the role of mechanotransduction in tumor development and cancer progression/metastasis, the mechanistic basis of force‐activated carcinogenesis is still enigmatic. Here, in this review, we have discussed the various cues and molecular connections to better comprehend the cellular mechanotransduction pathway, and we also explored the detailed role of some of the multiple players (proteins and macromolecular complexes) involved in mechanotransduction. Thus, we have described an avenue: how mechanical stress directs the epigenetic modifiers to modulate the epigenome of the cells and how aberrant stress leads to the cancer phenotype.

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“…Typically, as shown in Table 2, AFM provides high-resolution surface morphology information and, when integrated with scanning electron microscopy (SEM) or transmission electron microscopy (TEM), SEM and TEM offer structural and elemental composition information, resulting in more comprehensive characterization [102,103]. Combined with fluorescence spectroscopy, chemical composition information is provided [104], which is useful for studying the properties of biological and other organic membranes. Integration with Fourier-transform infrared spectroscopy (FTIR) [105] produces information on chemical composition, which enables in situ analysis of the molecular structure, bonding, and distribution on the membrane surface, and further reveals the chemical characteristics and mechanisms of membrane fouling.…”

Section: Potential Of Afm Coupled With Other Techniquesmentioning

confidence: 99%

Employing Atomic Force Microscopy (AFM) for Microscale Investigation of Interfaces and Interactions in Membrane Fouling Processes: New Perspectives and Prospects

Wei,

Zhang,

Wang

et al. 2024

Membranes

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Membrane fouling presents a significant challenge in the treatment of wastewater. Several detection methods have been used to interpret membrane fouling processes. Compared with other analysis and detection methods, atomic force microscopy (AFM) is widely used because of its advantages in liquid-phase in situ 3D imaging, ability to measure interactive forces, and mild testing conditions. Although AFM has been widely used in the study of membrane fouling, the current literature has not fully explored its potential. This review aims to uncover and provide a new perspective on the application of AFM technology in future studies on membrane fouling. Initially, a rigorous review was conducted on the morphology, roughness, and interaction forces of AFM in situ characterization of membranes and foulants. Then, the application of AFM in the process of changing membrane fouling factors was reviewed based on its in situ measurement capability, and it was found that changes in ionic conditions, pH, voltage, and even time can cause changes in membrane fouling morphology and forces. Existing membrane fouling models are then discussed, and the role of AFM in predicting and testing these models is presented. Finally, the potential of the improved AFM techniques to be applied in the field of membrane fouling has been underestimated. In this paper, we have fully elucidated the potentials of the improved AFM techniques to be applied in the process of membrane fouling, and we have presented the current challenges and the directions for the future development in an attempt to provide new insights into this field.

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Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy

Cited by 10 publications

References 130 publications

AFM is used to study the biophysics of hypertension‐induced tachyarrhythmia

AFM is used to study the biophysics of hypertension‐induced tachyarrhythmia

Mechanotransduction and epigenetic modulations of chromatin: Role of mechanical signals in gene regulation

Employing Atomic Force Microscopy (AFM) for Microscale Investigation of Interfaces and Interactions in Membrane Fouling Processes: New Perspectives and Prospects

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