Targeting biofilms in medical devices using probiotic cells: a systematic review

Carvalho, Fábio M.; Teixeira‐Santos, Rita; Mergulhão, Filipe; Gomes, Luciana C.

doi:10.3934/matersci.2021031

Cited by 10 publications

(20 citation statements)

References 99 publications

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“…With the widespread use of urinary tract devices, the formation of microbial biofilms on their surfaces contributes to the increasing number of urinary infections. Recently, some studies have proposed the use of probiotics and their metabolites as a reliable option to inhibit pathogenic biofilm growth and/or to disperse pre-formed biofilms in human subjects [23]. Although probiotics may exert different effects against the activity of pathogenic bacteria (displacement, exclusion, and competition), avoiding the initial attachment of pathogens by coating the surfaces seems to be the best strategy to fight biofilm-based infections [22].…”

Section: Discussionmentioning

confidence: 99%

“…Probiotics were recently introduced as an interesting option to inhibit or delay the onset of biofilm formation in medical devices [20]. The activity of probiotics and/or their isolated metabolites against medical device-associated infections, including UTDs, was recently reviewed [21][22][23]. Probiotics are beneficial live microorganisms that, when ingested in sufficient numbers, produce health benefits for the host [24].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Lactobacillus plantarum Biofilms on the Adhesion of Escherichia coli to Urinary Tract Devices

et al. 2021

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Novel technologies to prevent biofilm formation on urinary tract devices (UTDs) are continually being developed, with the ultimate purpose of reducing the incidence of urinary infections. Probiotics have been described as having the ability to displace adhering uropathogens and inhibit microbial adhesion to UTD materials. This work aimed to evaluate the effect of pre-established Lactobacillus plantarum biofilms on the adhesion of Escherichia coli to medical-grade silicone. The optimal growth conditions of lactobacilli biofilms on silicone were first assessed in 12-well plates. Then, biofilms of L. plantarum were placed in contact with E. coli suspensions for up to 24 h under quasi-static conditions. Biofilm monitoring was performed by determining the number of culturable cells and by confocal laser scanning microscopy (CLSM). Results showed significant reductions of 76%, 77% and 99% in E. coli culturability after exposure to L. plantarum biofilms for 3, 6 and 12 h, respectively, corroborating the CLSM analysis. The interactions between microbial cell surfaces and the silicone surface with and without L. plantarum biofilms were also characterized using contact angle measurements, where E. coli was shown to be thermodynamically less prone to adhere to L. plantarum biofilms than to silicone. Thus, this study suggests the use of probiotic cells as potential antibiofilm agents for urinary tract applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Lactobacillus plantarum Biofilms on the Adhesion of Escherichia coli to Urinary Tract Devices

et al. 2021

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“…Device-associated urinary tract infections are a critical problem caused by the high propensity of medical devices to microbial colonization. Previous studies have proposed the use of probiotics as a useful strategy to control pathogenic biofilms, demonstrating that probiotics cells and metabolites can displace adhering uropathogens from urinary devices materials and block bacterial adhesion to uroepithelial cells [ 47 , 48 ]. Probiotics can exert their antibiofilm activity by adopting different strategies: displacement, exclusion, and competition [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, our research group evaluated the ability of L. plantarum biofilms to prevent E. coli adhesion and biofilm formation on silicone rubber, following an exclusion strategy [ 42 ]. In the present work, the potential of two probiotic strains ( L. plantarum and L. rhamnosus ) to disperse pre-formed biofilms of E. coli and S. aureus under physiologically relevant conditions was assessed by adopting a displacement strategy [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…Each probiotic strain has multiple and diverse impacts on the host [ 36 ]. To date, several studies demonstrated the ability of probiotics to produce antimicrobial metabolites to manage biofilm infections [ 43 , 44 , 45 ], and their inhibitory effects on biofilm formation were extensively reviewed [ 46 , 47 , 48 ]. Moreover, probiotics were described to suppress quorum-sensing and affect biofilm integrity [ 46 ] by repressing the expression of biofilm-associated genes [ 49 ].…”

Section: Introductionmentioning

confidence: 99%

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Using Lactobacilli to Fight Escherichia coli and Staphylococcus aureus Biofilms on Urinary Tract Devices

2021

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The low efficacy of conventional treatments and the interest in finding natural-based approaches to counteract biofilm development on urinary tract devices have promoted the research on probiotics. This work evaluated the ability of two probiotic strains, Lactobacillus plantarum and Lactobacillus rhamnosus, in displacing pre-formed biofilms of Escherichia coli and Staphylococcus aureus from medical-grade silicone. Single-species biofilms of 24 h were placed in contact with each probiotic suspension for 6 h and 24 h, and the reductions in biofilm cell culturability and total biomass were monitored by counting colony-forming units and crystal violet assay, respectively. Both probiotics significantly reduced the culturability of E. coli and S. aureus biofilms, mainly after 24 h of exposure, with reduction percentages of 70% and 77% for L. plantarum and 76% and 63% for L. rhamnosus, respectively. Additionally, the amount of E. coli biofilm determined by CV staining was maintained approximately constant after 6 h of probiotic contact and significantly reduced up to 67% after 24 h. For S. aureus, only L. rhamnosus caused a significant effect on biofilm amount after 6 h of treatment. Hence, this study demonstrated the potential of lactobacilli to control the development of pre-established uropathogenic biofilms.

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Biofilms: cities of microorganisms

Prakash

Rayasam²,

Chaitanya³

et al. 2023

Bacterial Survival in the Hostile Environment

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Targeting biofilms in medical devices using probiotic cells: a systematic review

Cited by 10 publications

References 99 publications

Effect of Lactobacillus plantarum Biofilms on the Adhesion of Escherichia coli to Urinary Tract Devices

Effect of Lactobacillus plantarum Biofilms on the Adhesion of Escherichia coli to Urinary Tract Devices

Using Lactobacilli to Fight Escherichia coli and Staphylococcus aureus Biofilms on Urinary Tract Devices

Biofilms: cities of microorganisms

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