Targeting and detecting cancer cells using spontaneously formed multifunctional dendrimer-stabilized gold nanoparticles

Shi, Xiangyang; Wang, Su He; Antwerp, Mary E. Van; Chen, Xisui; Baker, James R.

doi:10.1039/b902199j

Cited by 81 publications

(65 citation statements)

References 39 publications

(65 reference statements)

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“…By NMR integration, the practical number of FI moieties attached onto each dendrimer was calculated to be 5.4 (Fig. S2, Supporting Information), similar to our previous studies [5,60,61]. Then, dual functional PEGylated a-TOS (characterized with 0.8 a-TOS moieties linked to each PEG via 1 H NMR, Fig.…”

Section: Synthesis and Characterization Of A-tos-conjugated Multifuncmentioning

confidence: 98%

Targeted cancer theranostics using alpha-tocopheryl succinate-conjugated multifunctional dendrimer-entrapped gold nanoparticles

et al. 2014

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Section: Synthesis and Characterization Of A-tos-conjugated Multifuncmentioning

confidence: 98%

Targeted cancer theranostics using alpha-tocopheryl succinate-conjugated multifunctional dendrimer-entrapped gold nanoparticles

et al. 2014

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“…[15,18,19] Among the many types of polymers used to synthesize Au NPs, [20][21][22][23] poly(amidoamine) (PAMAM) dendrimers, a family of highly branched, monodispersed, and synthetic macromolecules, [24] have been used as facile templates or stabilizers to generate dendrimer-entrapped Au NPs (Au DENPs) [14,[25][26][27] or dendrimer-stabilized Au NPs (Au DSNPs). [13,[28][29][30][31][32] Au DENPs refers to a nanostructure with one or more Au NPs entrapped within one dendrimer molecule with an Au NP size usually smaller than 5 nm, whereas Au DSNPs refers to a nanostructure with one Au NP surrounded with multiple dendrimers with an Au NP size generally larger than 5 nm. [28] Some Au DENPs or Au DSNPs have been used for CT imaging applications.…”

Section: Introductionmentioning

confidence: 99%

“…[26] Ligand-modified generation 5 PAMAM dendrimers can be used as templates or stabilizers to form Au DENPs or Au DSNPs for tumor microvasculature targeting [34] or to target cancer cells over-expressing the FA receptors. [32] A very recent study has shown that FA-modified Au DENPs can be used for targeted CT imaging of human lung adenocarcinoma. [19] However, in most of these studies, generation 5 (G5) dendrimers have been used as either templates or stabilizers to form Au DENPs or DSNPs, which limits their practical CT imaging applications due to the high cost of the commercially available high generation dendrimers.…”

Section: Introductionmentioning

confidence: 99%

Targeted Tumor Computed Tomography Imaging Using Low‐Generation Dendrimer‐Stabilized Gold Nanoparticles

Liu

Wen

et al. 2013

Chemistry A European J

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We report a facile approach to fabricating low-generation poly(amidoamine) (PAMAM) dendrimer-stabilized gold nanoparticles (Au DSNPs) functionalized with folic acid (FA) for in vitro and in vivo targeted computed tomography (CT) imaging of cancer cells. In this study, amine-terminated generation 2 PAMAM dendrimers were employed as stabilizers to form Au DSNPs without additional reducing agents. The formed Au DSNPs with an Au core size of 5.5 nm were covalently modified with the targeting ligand FA, followed by acetylation of the remaining dendrimer terminal amines to endow the particles with targeting specificity and improved biocompatibility. Our characterization data show that the formed FA-modified Au DSNPs are stable at different pH values (5-8) and temperatures (4-50 °C), as well as in different aqueous media. MTT assay data along with cell morphology observations reveal that the FA-modified Au DSNPs are noncytotoxic in the particle concentration range of 0-3000 nM. X-ray attenuation coefficient measurements show that the CT value of FA-modified Au DSNPs is much higher than that of Omnipaque (a clinically used CT contrast agent) at the same concentration of the radiodense elements (Au or iodine). Importantly, the FA-modified Au DSNPs are able to specifically target a model cancer cell line (KB cells, a human epithelial carcinoma cell line) over-expressing FA receptors and they enable targeted CT imaging of the cancer cells in vitro and the xenografted tumor model in vivo after intravenous administration of the particles. With the simple synthesis approach, easy modification, good cytocompatibility, and high X-ray attenuation coefficient, the FA-modified low-generation Au DSNPs could be used as promising contrast agents for targeted CT imaging of different tumors over-expressing FA receptors.

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“…There has been considerable interest in dendimer-based nanotechnology for targeted cancer imaging and therapy. Folatefunctionalized dendimer-entrapped gold nanoparticles have been reported as a unique nanoplatform for targeting and imaging of a variety of biological systems (Shi et al 2009). These nanoplatforms have been shown to specifically bind to FR-expressing cells followed by their internalization into lysozomes (Shi et al 2007).…”

Section: Resultsmentioning

confidence: 99%

Cancer active targeting by nanoparticles: a comprehensive review of literature

Bazak

Houri

Achy

et al. 2014

J Cancer Res Clin Oncol

559

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Purpose Cancer is one of the leading causes of death, and thus, the scientific community has but great efforts to improve cancer management. Among the major challenges in cancer management is development of agents that can be used for early diagnosis and effective therapy. Conventional cancer management frequently lacks accurate tools for detection of early tumors and has an associated risk of serious side effects of chemotherapeutics. The need to optimize therapeutic ratio as the difference with which a treatment affects cancer cells versus healthy tissues lead to idea that it is needful to have a treatment that could act a the “magic bullet”—recognize cancer cells only. Nanoparticle platforms offer a variety of potentially efficient solutions for development of targeted agents that can be exploited for cancer diagnosis and treatment. There are two ways by which targeting of nanoparticles can be achieved, namely passive and active targeting. Passive targeting allows for the efficient localization of nanoparticles within the tumor microenvironment. Active targeting facilitates the active uptake of nanoparticles by the tumor cells themselves. Methods Relevant English electronic databases and scientifically published original articles and reviews were systematically searched for the purpose of this review. Results In this report, we present a comprehensive review of literatures focusing on the active targeting of nanoparticles to cancer cells, including antibody and antibody fragment-based targeting, antigen-based targeting, aptamer-based targeting, as well as ligand-based targeting. Conclusion To date, the optimum targeting strategy has not yet been announced, each has its own advantages and disadvantages even though a number of them have found their way for clinical application. Perhaps, a combination of strategies can be employed to improve the precision of drug delivery, paving the way for a more effective personalized therapy.

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Targeting and detecting cancer cells using spontaneously formed multifunctional dendrimer-stabilized gold nanoparticles

Cited by 81 publications

References 39 publications

Targeted cancer theranostics using alpha-tocopheryl succinate-conjugated multifunctional dendrimer-entrapped gold nanoparticles

Targeted cancer theranostics using alpha-tocopheryl succinate-conjugated multifunctional dendrimer-entrapped gold nanoparticles

Targeted Tumor Computed Tomography Imaging Using Low‐Generation Dendrimer‐Stabilized Gold Nanoparticles

Cancer active targeting by nanoparticles: a comprehensive review of literature

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