Targeting adenovirus

A rapid and efficient method for purification of recombinant adenovirus with arginine–glycine–aspartic acid-modified fibers

“…After attachment, the virion is then internalized by endocytosis through the interaction of its penton base with α v β 3 and α v β 5 integrins on the host cell surface [3]. However, there is growing evidence that the expression of CAR is frequently suppressed in various types of cells, including tumor cells and in primary tumors [4,5], resulting in resistance to adenovirus infection [6][7][8] Unfortunately, technical difficulties may be encountered in production of certain modified adenovectors. We have encountered difficulties when producing certain adenovectors by this method, especially those with RGD-modified fibers or "sticky" virus.…”

“…After attachment, the virion is then internalized by endocytosis through the interaction of its penton base with α v β 3 and α v β 5 integrins on the host cell surface [3]. However, there is growing evidence that the expression of CAR is frequently suppressed in various types of cells, including tumor cells and in primary tumors [4,5], resulting in resistance to adenovirus infection [6][7][8]. Nevertheless, various studies have shown that adenovectors with modified capsid proteins, such as additions of polylysine or RGD sequence to fibers, can effectively infect cells with defective CAR expression [9][10][11].…”

Employment of microarray analysis to characterize biologic differences associated with tropism-modified adenoviral vectors: utilization of non-native cellular entry pathways

“…The predominant factor limiting the efficacy of Ad-based clinical interventions is anti-vector immunity. A contributing factor limiting the efficacy of Ad infection has been shown to be paucity of expression of the coxsackie and adenovirus receptor or CAR, which is the primary cellular receptor for serotype 5 adenovirus, on some target cells such as smooth muscle, hematopoietic cells 4 and advanced cancer. 2,5 In this regard, retargeting strategies which redirect Ad to alternative cell receptors have been developed.…”

Potent antitumor efficacy of XAF1 delivered by conditionally replicative adenovirus vector via caspase-independent apoptosis

“…30 Many measures have been taken to construct safer viral vectors for gene delivery. For instance, transductional targeting (e.g., through genetic modification of the viral capsid [31][32][33] ) or transcriptional targeting (e.g., using tumor-selective promoters to control foreign gene expression) may offer additional safety features in regulating the long-term expression. Gutless, the third generation adenovirus, lacks all viral coding regions and consequently exhibit low toxicity and immunogenicity, thus permitting prolonged expression of the transgene.…”