Targeting a gene regulatory element enhances rice grain yield by decoupling panicle number and size

Song, Xiaoguang; Meng, Xiangbing; Guo, Hongyan; Qiao, Chengpeng; Jing, Yanhui; Chen, Mingjiang; Liu, Guifu; Wang, Bing; Wang, Yonghong; Li, Jiayang; Yu, Hong

doi:10.1038/s41587-022-01281-7

Cited by 126 publications

(67 citation statements)

References 46 publications

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“…In this study, we revealed that the functional differences of the two major GmST05 haplotypes resulted from natural variations in the promoter regions that affected their transcriptional level differences. Modulating the expression of important genes using genome editing will be a powerful approach for accelerating crop breeding (Hendelman et al., 2021； Wang et al., 2021； Song et al., 2022). Therefore, a modification of the transcription‐regulating regions of GmST05 may help us improve soybean yield.…”

Section: Discussionmentioning

confidence: 99%

Natural allelic variation of GmST05 controlling seed size and quality in soybean

Duan

Zhang

et al. 2022

Plant Biotechnology Journal

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Seed size is one of the most important agronomic traits determining the yield of crops. Cloning the key genes controlling seed size and pyramiding their elite alleles will facilitate yield improvement. To date, few genes controlling seed size have been identified in soybean, a major crop that provides half of the plant oil and one quarter of the plant protein globally. Here, through a genome-wide association study of over 1800 soybean accessions, we determined that natural allelic variation at GmST05 (Seed Thickness 05) predominantly controlled seed thickness and size in soybean germplasm. Further analyses suggested that the two major haplotypes of GmST05 differed significantly at the transcriptional level. Transgenic experiments demonstrated that GmST05 positively regulated seed size and influenced oil and protein contents, possibly by regulating the transcription of GmSWEET10a. Population genetic diversity analysis suggested that allelic variations of GmST05 were selected during geographical differentiation but have not been fixed. In summary, natural variation in GmST05 determines transcription levels and influences seed size and quality in soybean, making it an important gene resource for soybean molecular breeding.

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Section: Discussionmentioning

confidence: 99%

Natural allelic variation of GmST05 controlling seed size and quality in soybean

Duan

Zhang

et al. 2022

Plant Biotechnology Journal

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“…7 a; Additional file 1 : Figure S6), stress-inducible promoters like RD29A and tissue-specific promoters from plant genes could be used instead of 35S promoter during genetic improvement of grapevine and other crops in the future. Recently, the CRISPR/Cas9 has been used to improve the crop traits by editing the regulatory elements/promoters of genes of interest [ 50 , 51 ]. Elevated ABA receptor expression could be achieved through promoter editing by using CRISPR/Cas9 technology.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of grape ABA receptor gene VaPYL4 enhances tolerance to multiple abiotic stresses in Arabidopsis

et al. 2022

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Background Abscisic acid (ABA) plays a crucial role in abiotic stress responses. The pyrabactin resistance (PYR)/PYR-like (PYL)/regulatory component of ABA receptor (RCAR) proteins that have been characterized as ABA receptors function as the core components in ABA signaling pathway. However, the functions of grape PYL genes in response to different abiotic stresses, particularly cold stress, remain less studied. Results In this study, we investigated the expression profiles of grape PYL genes upon cold treatment and isolated the VaPYL4 gene from Vitis amurensis, a cold-hardy grape species. Overexpression of VaPYL4 gene in grape calli and Arabidopsis resulted in enhanced cold tolerance. Moreover, plant resistance to drought and salt stress was also improved by overexpressing VaPYL4 in Arabidopsis. More importantly, we evaluated the contribution of VaPYL4 to plant growth and development after the treatment with cold, salt and drought stress simultaneously. The transgenic plants showed higher survival rates, earlier flowering phenotype, and heavier fresh weight of seedlings and siliques when compared with wild-type plants. Physiological analyses showed that transgenic plants had much lower content of malondialdehyde (MDA) and higher peroxidase (POD) activity. Stress-responsive genes such as RD29A (Responsive to desiccation 29A), COR15A (Cold responsive 15A) and KIN2 (Kinase 2) were also significantly up-regulated in VaPYL4-overexpressing Arabidopsis plants. Conclusions Our results show that overexpression of VaPYL4 could improve plant performance upon different abiotic stresses, which therefore provides a useful strategy for engineering future crops to deal with adverse environments.

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“…To clone the genes underlying these loci, a chromosome segment substitution lines (CSSL) population should be constructed for the fine mapping of each related QTL [ 19 ]. Precise genome editing techniques will benefit the utilization of genes that control plant architecture in a selection of ideal varieties [ 42 ], provide strategies to reduce gene pleiotropy through the modification of cis -regulatory regions [ 43 ] or manipulate target genes for the de novo domestication of wild crop relatives [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Genomic Regions Associated with Vine Growth and Plant Height of Soybean

Zhang

Guo

et al. 2022

IJMS

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Vining growth (VG) and high plant height (PH) are the physiological traits of wild soybean that preclude their utilization for domesticated soybean breeding and improvement. To identify VG- and PH-related quantitative trait loci (QTLs) in different genetic resources, two populations of recombinant inbred lines (RILs) were developed by crossing a cultivated soybean, Zhonghuang39 (ZH39), with two wild soybean accessions, NY27-38 and NY36-87. Each line from the two crosses was evaluated for VG and PH. Three QTLs for VG and three for PH, detected in the ZH39 × NY27-38 population of the RILs, co-located on chromosomes 2, 17 and 19. The VG- and PH-related QTL in the ZH39 × NY36-87 population co-located on chromosome 19. A common QTL shared by the two populations was located on chromosome 19, suggesting that this major QTL was consistently selected for in different genetic backgrounds. The results suggest that different loci are involved in the domestication or adaptations of soybean of various genetic backgrounds. The molecular markers presented here would benefit the fine mapping and cloning of candidate genes underlying the VG and PH co-localized regions and thus facilitate the utilization of wild resources in breeding by avoiding undesirable traits.

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Targeting a gene regulatory element enhances rice grain yield by decoupling panicle number and size

Cited by 126 publications

References 46 publications

Natural allelic variation of GmST05 controlling seed size and quality in soybean

Natural allelic variation of GmST05 controlling seed size and quality in soybean

Overexpression of grape ABA receptor gene VaPYL4 enhances tolerance to multiple abiotic stresses in Arabidopsis

Identification of Genomic Regions Associated with Vine Growth and Plant Height of Soybean

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