Targeted use of fluorescence in situ hybridization (FISH) in cytospin preparations

Abstract

BACKGROUND: Fluorescence in situ hybridization (FISH) results from fine needle aspirates (FNA) of B‐cell non‐Hodgkin lymphomas (NHLs) were reviewed to 1) investigate the value added by using specific gene rearrangement probes to lymphoma diagnosis, prognosis, and subtyping; and 2) evaluate the prevalence of cytogenetic alterations other than specific translocations. METHODS: FISH results from assays performed on cytospin preparations from NHL FNAs over a 6‐year period (2003‐2009) were selected. Immunophenotyping, clinical data, and cytomorphologic data were reviewed according to the current World Health Organization (WHO) classification system. Hybridized probes, the purpose for the assay (subtyping or prognosis), and the cytogenetic abnormalities observed were retrieved from cytology reports. Data was categorized according to specific rearrangements and other chromosomal abnormalities. RESULTS: Successful results were obtained in 284 (95.3%) of 298 cases from 282 patients. Abnormalities were found in 216 (76%) cases and 68 (24%) did not show alteration. Among cases submitted for subtyping, 198 showed FISH‐positive results, and specific gene rearrangements were found in 122 (61.6%) cases as follows: follicular 82, mantle cell 21, marginal zone 3, “dual hit” 13, and Burkitt lymphoma 3. In 21 cases, abnormalities were useful for prognosis. Nonspecific alterations alone or in combination with translocations were found in 98 cases. CONCLUSIONS: FISH performed on cytospin preparations was useful for confirmation of specific subclasses of NHL and may also provide valuable prognostic information. Cytogenetic abnormalities other than specific translocations were frequently found and could provide supportive evidence for a definitive diagnosis of lymphoma in FNA. Cancer (Cancer Cytopathol) 2010. © 2010 American Cancer Society.

Cytomorphologic findings of B‐cell lymphomas with concurrent IGH/BCL2 and MYC rearrangements (dual‐translocation lymphomas)

“…The detailed protocol, the monoclonal antibodies, and the method of assessment used have been reported previously. 13 …”

“…The detailed protocol used has been detailed previously. 13 Research ethics approval for this study was sought and obtained from the Human Tissue Committee at the University Health Network.…”

Role of endoscopic ultrasonography with and without fine needle aspiration cytology in the diagnosis and staging of lymphoma

“…They include specific chromosomal translocations in: follicular lymphoma, mantle cell lymphoma, anaplastic large cell lym-phoma, Burkitt lymphoma, and MALT. A recent study, using percutaneous FNA assessed the sensitivity of fluorescence in situ hybridization (FISH) in lymphoma sub-typing [58] . FISH was successful in 95.3% of the cases and sub-typing was possible in 61.6%.…”

Maximizing the yield of lymph node cytology

“…5 CPs that are created by preserving whole intact nuclei provide a good alternative to CB sections and avoid the nuclear truncation artifacts inherent in the sectioning of FFPE CBs. In addition, using CPs for FISH studies can save all possible FFPE CB sections for immunohistochemistry.…”