Targeted tumor detection: guidelines for developing biotinylated diagnostics

Jang, Jin Woo; Kim, Woo Ri; Sharma, Amit; Cho, Suk Hee; James, Tony D.; Kang, Chulhun; Kim, Jong Seung

doi:10.1039/c7cc00311k

Cited by 13 publications

(7 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To prove the enhanced uptake of conjugate 1 by HeLa cells, which possess overexpressed levels of α v β 3 integrins, , the cRGD binding assay was performed by adopting the described protocol . In the process, the cells were pre-incubated with various cRGD peptide concentrations (0–100 μM) and then treated with conjugate 1 (10 μM).…”

Section: Resultsmentioning

confidence: 99%

AminoBODIPY Conjugates for Targeted Drug Delivery Systems and Real-Time Monitoring of Drug Release

et al. 2021

View full text Add to dashboard Cite

In this work, we report two concepts of drug delivery based on small-molecule drug conjugates with the ability of specific targeting and drug release monitoring via ratiometric fluorescence. The functionality of these concepts has been verified by two model systems consisting of three parts: (i) fluorescent aminoBODIPY for real-time detection of conjugate cleavage, (ii) a c(RGDfK) peptide specific for α v β 3 integrin receptors targeting angiogenesis in most solid tumors or redBODIPY for conjugate cleavage monitoring via FRET, and (iii) pegylated-2-phenyl-3-hydroxy-4(1H)-quinolinone (3HQ) as a model drug. The model drug release is based on a self-immolative disulfide linker sensitive to environments containing thiols, especially glutathione, which is overexpressed in cancer cells. The results show effective thiol-mediated cleavage of the fluorescent reporter and the subsequent liberation of the drug in a tube. The conjugate with c(RGDfK) was confirmed to penetrate the cells via interaction with integrin receptors. Drug release from this conjugate is possible to monitor inside the cells. Further, the synthetic approach to the conjugates and the method of fluorescence monitoring of the drug release have also been described.

show abstract

Section: Resultsmentioning

confidence: 99%

AminoBODIPY Conjugates for Targeted Drug Delivery Systems and Real-Time Monitoring of Drug Release

et al. 2021

View full text Add to dashboard Cite

show abstract

“…44 Fluorescent probes incorporating other ligand molecules such as biotin to actively target a membrane receptor have also been developed. 48 To corroborate the promise of ligand-attached fluorogenic probes for the study of chemical biology and disease diagnosis, targeted detection of trace amount of endogenous species implicated in important physiologic or pathologic cellular pathways must be achieved.…”

Section: Small-molecular Fluorescent Probes For Receptor Sensing and Targeted Bioimagingmentioning

confidence: 99%

Lightening Up Membrane Receptors with Fluorescent Molecular Probes and Supramolecular Materials

Tian

2018

Chem

View full text Add to dashboard Cite

Membrane receptors, among other membrane proteins that universally exist on the cell surface, are a class of important macromolecules responsible for cellsignaling processes. They are activated or inhibited through selective binding with endogenous ligand molecules. These binding activities are implicated in physiologic as well as pathologic events if errors in signaling interactions occur. The thorough understanding of receptor-ligand recognition is of paramount importance for fundamental biological studies and, in particular, could improve the precision of disease diagnosis and therapy. However, current techniques capable of tracking the dynamic actions of membrane receptors are elusive. This review highlights our recent progress in the development of fluorescent probes and supramolecular materials for receptor-targeting biosensing and bioimaging. Strategies in probe construction and the practical biomedical applications that have been achieved are discussed. Perspectives and challenges with respect to this interdisciplinary field are offered.

show abstract

“…Targeted fluorescence molecular probes have been developed to explore various biological processes involved in cancer and at cellular levels. − Various fluorescent probes have been developed by several research groups for imaging certain overexpressed enzymes as target for cancer selective imaging. Particularly, quinone oxidoreductase isozyme 1 (NQO1), β-galactosidase, and γ-glutamyltranspeptidase (GGT) has been explored as a target to differentiate cancer cells from normal cells. − Likewise, other chemical events occurring specifically in cancer cells, like acidic pH and overproduction of H 2 O 2 and glutathione, have been explored. − These research efforts have widened the knowledge and ideas for further development of molecular targeted probes.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Probe for Monitoring Hydrogen Peroxide in COX-2-Positive Cancer Cells

Lee

Kim

Jangili

et al. 2020

ACS Appl. Bio Mater.

Self Cite

View full text Add to dashboard Cite

Hydrogen peroxide (H 2 O 2 ), an important marker for oxidative stress, plays a vital role in cellular biological functions. Overproduction of H 2 O 2 causes oxidative damage to cellular functions and promotes cancer and other neurodegenerative diseases. Also, cyclooxygenase-2 (COX-2) enzyme is known to be expressed in several cancer types and exerts multifaceted roles in carcinogenesis and resistance to cancer treatment. Hence, it is important to monitor the H 2 O 2 concentration changes in the COX-2-expressing cancer cells. Herein, we have developed a molecular fluorescent ratiometric H 2 O 2 -responsive probe (NPDIN) composed of indomethacin (COX-2 inhibitor) conjugated with 1,8-napthalimide boronate ester as fluorescent reporter through a chemical linker. The probe was capable of imaging the endogenous H 2 O 2 in COX-2 overexpressing cancer cell lines (A549, LoVo, HT29, and Caco-2). Further studies revealed the critical role of the indomethacin moiety in the cellular uptake behavior of NPDIN in COX-2overexpressing cancer cells. Collectively, our results demonstrated NPDIN as a COX-2-positive cancer-targeting sensitive ratiometric fluorescent probe (I 554 /I 398 ) for H 2 O 2 imaging and showed its promising biological applications in the future.

show abstract

Targeted tumor detection: guidelines for developing biotinylated diagnostics

Abstract: The challenge in achieving precision medicine relies on how to advance and/or enhance new as well as old therapeutic strategies. Here, we highlight the significant role hydrophilicity of biotinylated fluorescent probe's plays on their cellular uptake behaviour.

Cited by 13 publications

References 38 publications

AminoBODIPY Conjugates for Targeted Drug Delivery Systems and Real-Time Monitoring of Drug Release

AminoBODIPY Conjugates for Targeted Drug Delivery Systems and Real-Time Monitoring of Drug Release

Lightening Up Membrane Receptors with Fluorescent Molecular Probes and Supramolecular Materials

Fluorescent Probe for Monitoring Hydrogen Peroxide in COX-2-Positive Cancer Cells

Contact Info

Product

Resources

About