Targeted suppression of siRNA biogenesis in Arabidopsis pollen promotes triploid seed viability
Kannan Pachamuthu,
Matthieu Simon,
Filipe Borges
Abstract:In plants, small-interfering RNAs (siRNAs) mediate epigenetic silencing via the RNA-directed DNA methylation (RdDM) pathway, which is particularly prominent during reproduction and seed development. However, there is limited understanding of the origins and dynamics of reproductive siRNAs acting in different cellular and developmental contexts. Here, we used the RNaseIII-like protein RTL1 to suppress siRNA biogenesis in Arabidopsis pollen, and found distinct siRNA subsets produced during pollen development. We… Show more
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