Targeted sphingolipidomics indicates increased C22-C24:16 ratios of virtually all assayed classes in liver, kidney, and plasma of fumonisin-fed chickens

Lassallette, Elodie; Collén, Pi Nyvall; Guerre, Philippe

doi:10.1016/j.ecoenv.2023.115697

Cited by 4 publications

(6 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the effects of fumonisins on the C22-24:C16 ratios were similar irrespective of the total sphingolipid contents measured at the classes level. This observation is consistent with previous work [27]. It is also consistent with the fact that the Sa:So ratio is preferable to sphinganine to detect fumonisins exposure [7,39].…”

Section: Discussionsupporting

confidence: 93%

“…The effect of the FB diet on the C22-24:C16 ratios was mainly due to a reduction in C16 sphingolipids. These results are in agreement with previous ndings in chicken liver and kidney [23,24,27]. The C22-24:C16 ratios, recalculated from data reported in turkey liver [21], were also increased after feeding a diet containing fumonisins.…”

Section: Discussionsupporting

confidence: 92%

“…The lack of a signi cant effect of fumonisins on the ceramide levels in ducks could be due to the low FB1 content measured in the liver, which was only 0.018 µmol/kg. This observation is in agreement with previous data in chickens, where a slight decrease in the Cer level was observed in the liver at an FB1 concentration of 0.029 µmol/kg, whereas a concentration of 0.011 µmol/kg had no effect on Cer levels in the kidney [27].…”

Section: Effect Of Fusariotoxins On Ceramides Glycosylceramides and S...supporting

confidence: 93%

“…Chromatograms were analysed using Agilent MassHunter quantitative analysis software with quadratic calibration using a 1/x² weighting factor. The linearity of the method was good over a wide range of concentrations (Table S2), consistent with previous results [27,36]. The accuracy was considered acceptable, with a relative standard deviation (RSD) of 20%.…”

Section: Analysis Of Sphingolipidssupporting

confidence: 89%

“…Several studies in chickens and turkeys have shown that administration of fumonisins leads to a decrease in long-chain ceramides and an increase in very-longchain ceramides in the liver [21][22][23][24]. Very-long-chain Cer (incorporating a fatty acid of 22 to 26 carbon atoms, C22-26) are mainly synthesised by CerS type 2, whereas long-chain Cer (incorporating a fatty acid of 14 to 16 carbon atoms, C14-16) are synthesised in the liver by CerS type 5 [25][26][27][28]. The C22-24:C16 sphingolipid ratios were hence proposed as a complementary biomarker to the Sa:So ratio in the characterisation of the effects of fumonisins on the sphingolipidome [27].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Fumonisins alone or mixed with other fusariotoxins increase the C22–24:C16 sphingolipid ratios in chickens and ducks, while deoxynivalenol and zearalenone have no effect

Guerre,

Lassallette,

Beaujardin-Daurian

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Background The inhibitory effect of fumonisins on ceramide synthases results in several effects on the sphingolipidome, and recent results in chickens suggest that the C22–24:C16 sphingolipid ratios may be complementary biomarkers to Sa:So. Feeding diets containing fumonisins, deoxynivalenol, or zearalenone alone or in combination up to the maximum levels recommended by the European Commission for 35 days in chickens and 12 days in ducks resulted in no effects on performance or toxicity. The aim of this study was to investigate their effects on the liver sphingolipidome. Results Sphingolipids were characterised by UHPLC-MSMS. Numerous significant effects of fusariotoxins on sphingolipids were observed at the class and analyte levels. Fusariotoxins alone decreased sphingolipids in chickens but had weak effects in ducks. Feeding fumonisins in combination with deoxynivalenol and zearalenone increased sphingolipid levels in chickens but not in ducks. These increases were mainly due to an increase in C22–24 sphingolipids, whereas C16 sphingolipids decreased. The Sa:So ratio and the C22–24:C16 ratio for ceramides, sphingomyelins, monohexosylceramides, dihydroceramides, and dihydrosphingomyelins were unaffected in chickens and ducks fed the diets containing deoxynivalenol or zearalenone but increased in animals fed the fumonisin diets. Interestingly, while the effects of feeding fumonisins alone or in combination on the total amount of sphingolipids differed in chickens, the Sa:So and C22-24:C16 ratios were similar. Partial least squares-discriminant analysis of sphingolipids did not allow discrimination of the animals exposed to deoxynivalenol or zearalenone from the unexposed animals. By contrast, good discrimination of the animals fed the diets containing fumonisins was achieved. Sphinganine, d20 sphinganine, a large number of C16 sphingolipids, and dihydrosphingomyelins for which the fatty acid contains more than 18 carbon atoms, were important variables in the models obtained in chickens and ducks. Conclusions The C22–24:C16 ratios of sphingolipids are increased in chickens and ducks by intake of fumonisins but not deoxynivalenol or zearalenone. The increases were similar when fumonisins were fed alone or in combination with deoxynivalenol or zearalenone. The increase in the C22–24:C16 ratio may be an important factor in explaining the interspecies differences in sensitivity to fumonisin toxicity.

show abstract

Section: Discussionsupporting

confidence: 93%