Targeted Rehabilitation After Extracellular Matrix Scaffold Transplantation for the Treatment of Volumetric Muscle Loss

Gentile, Natalie; Stearns, Kristen M.; Brown, Elke H.P.; Rubin, J. Peter; Boninger, Michael L.; Dearth, Christopher L.; Ambrosio, Fabrisia; Badylak, Stephen F.

doi:10.1097/phm.0000000000000145

Cited by 68 publications

(55 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On a clinical setting application, commercially available ECM products have been successfully applied to restore chronic volumetric muscle injuries [11,94,96], reporting esthetic and, to some degree, functional improvements.…”

Section: Msc-biomaterials Systems For In Vivo Skeletal Muscle Regeneramentioning

confidence: 99%

“…In research scenarios, therapies are applied to recently injured sites. However, on a clinical setting, the most expectable situation is a chronic irreparable wound that underwent multiple surgical repair attempts though the course of several months or years [11,94,96]. Hence, as pointed out by Vigodarzere and Mantero [92], the homeostasis of such extensively injured and remodeled sites is significantly distinct from freshly induced insults; thus, the predictive value of the currently used animal models turns questionable.…”

Section: Cell Sourcementioning

confidence: 99%

See 1 more Smart Citation

Trends in Mesenchymal Stem Cells' Applications for Skeletal Muscle Repair and Regeneration

Caseiro¹,

Pereira²,

Bártolo³

et al. 2015

Progress in Stem Cell Transplantation

View full text Add to dashboard Cite

Skeletal muscle injuries are quite frequent in traumatic scenarios, such as war injuries or road-or work-related accidents. The skeletal muscle has good regenerative ability, but the extent or recurrence of muscle injury might impair complete structural and functional recovery. Severe tissue loss overwhelms skeletal muscle´s intrinsic regenerative capabilities and culminates in the development of noncontractile fibrous tissue scar. Conservative RICE -based and surgical treatments show limited efficacy in terms of improving these severe cases outcomes, pressing the need for new approaches on skeletal muscle's therapy. Since the first suggestions of the potential of mesenchymal stem cells for regenerative medicine and tissue engineering, many applications have been explored for a variety of tissues and diseases, including the skeletal muscle, which is the focus of this literature review.

show abstract

Section: Msc-biomaterials Systems For In Vivo Skeletal Muscle Regeneramentioning

confidence: 99%

Section: Cell Sourcementioning

confidence: 99%

Trends in Mesenchymal Stem Cells' Applications for Skeletal Muscle Repair and Regeneration

Caseiro¹,

Pereira²,

Bártolo³

et al. 2015

Progress in Stem Cell Transplantation

View full text Add to dashboard Cite

show abstract

“…Tissue engineering strategies aimed at regenerating lost tissue and mitigating chronic muscle function are being investigated for VML repair. More specifically, extracellular matrix (ECM) scaffolds have been largely investigated in preclinical VML injury models [Turner et al, 2010;Machingal et al, 2011;Turner et al, 2011;Sicari et al, 2012;Wolf et al, 2012;Chen et al, 2013;Corona et al, 2013b;Sicari et al, 2014;Aurora et al, 2015] and more recently in the clinic for lower-extremity VML repair [Mase et al, 2010;Gentile et al, 2014;Sicari et al, 2014]. These scaffolds have been derived from different species (e.g., porcine, rodent, and canine) and different sources, e.g., urinary bladder matrix (UBM), small intestinal submucosa, and skeletal muscle.…”

Section: Introductionmentioning

confidence: 99%

“…Herein, we compare the macrophage and angiogenic responses including the remodeling outcomes of an acellular ECM scaffold, more specifically, a commercially available porcine UBM scaffold, MatriStem TM , and autograft (autologous muscle graft) using a rodent VML injury model. The porcine UBM scaffold was selected as it has been used clinically for lowerextremity VML repair [Gentile et al, 2014;Sicari et al, 2014]. We hypothesize that when compared to autograft repair, the porcine UBM scaffold will not orchestrate the spatiotemporal cellular events necessary for appreciable muscle regeneration.…”

Section: Introductionmentioning

confidence: 99%

A Porcine Urinary Bladder Matrix Does Not Recapitulate the Spatiotemporal Macrophage Response of Muscle Regeneration after Volumetric Muscle Loss Injury

Aurora¹,

Corona²,

Walters³

2016

Cells Tissues Organs

View full text Add to dashboard Cite

Volumetric muscle loss (VML) results in irrecoverable loss of muscle tissue making its repair challenging. VML repair with acellular extracellular matrix (ECM) scaffolds devoid of exogenous cells has shown improved muscle function, but limited de novo muscle fiber regeneration. On the other hand, studies using minced autologous and free autologous muscle grafts have reported appreciable muscle regeneration. This raises the fundamental question whether an acellular ECM scaffold can orchestrate the spatiotemporal cellular events necessary for appreciable muscle fiber regeneration. This study compares the macrophage and angiogenic responses including the remodeling outcomes of a commercially available porcine urinary bladder matrix, MatriStem™, and autologous muscle grafts. The early heightened and protracted M1 response of the scaffold indicates that the scaffold does not recapitulate the spatiotemporal macrophage response of the autograft tissue. Additionally, the scaffold only supports limited de novo muscle fiber formation and regressing vessel density. Furthermore, scaffold remodeling is accompanied by increased presence of transforming growth factor and α-smooth muscle actin, which is consistent with remodeling of the scaffold into a fibrotic scar-like tissue. The limited muscle formation and scaffold-mediated fibrosis noted in this study corroborates the findings of recent studies that investigated acellular ECM scaffolds (devoid of myogenic cells) for VML repair. Taken together, acellular ECM scaffolds when used for VML repair will likely remodel into a fibrotic scar-like tissue and support limited de novo muscle fiber regeneration primarily in the proximity of the injured musculature. This is a work of the US Government and is not subject to copyright protection in the USA. Foreign copyrights may apply. Published by S. Karger AG, Basel

show abstract

“…To date, a total of eight VML cases have been reported among four studies [9,[20][21][22], but standardized assessments of VML magnitude and strength loss or disability are not consistently provided. Complicating the distinction of debilitating VML are the unique characteristics of various muscle units (e.g., quadriceps vs anterior tibialis muscles) and their recruitment during daily living and work activities.…”

Section: Discussionmentioning

confidence: 99%

Volumetric muscle loss leads to permanent disability following extremity trauma

et al. 2015

View full text Add to dashboard Cite

Abstract-Extremity injuries comprise the majority of battlefield injuries and contribute the most to long-term disability of servicemembers. The purpose of this study was to better define the contribution of muscle deficits and volumetric muscle loss (VML) to the designation of long-term disability in order to better understand their effect on outcomes for limb-salvage patients. Medically retired servicemembers who sustained a combat-related type III open tibia fracture (Orthopedic cohort) were reviewed for results of their medical evaluation leading to discharge from military service. A cohort of battlefield-injured servicemembers (including those with nonorthopedic injuries) who were medically retired because of various injuries (General cohort) was also examined. Muscle conditions accounted for 65% of the disability of patients in the Orthopedic cohort. Among the General cohort, 92% of the muscle conditions were identified as VML. VML is a condition that contributes significantly to long-term disability, and the development of therapies addressing VML has the potential to fill a significant void in orthopedic care.

show abstract

Targeted Rehabilitation After Extracellular Matrix Scaffold Transplantation for the Treatment of Volumetric Muscle Loss

Cited by 68 publications

References 4 publications

Trends in Mesenchymal Stem Cells' Applications for Skeletal Muscle Repair and Regeneration

Trends in Mesenchymal Stem Cells' Applications for Skeletal Muscle Repair and Regeneration

A Porcine Urinary Bladder Matrix Does Not Recapitulate the Spatiotemporal Macrophage Response of Muscle Regeneration after Volumetric Muscle Loss Injury

Volumetric muscle loss leads to permanent disability following extremity trauma

Contact Info

Product

Resources

About