Targeted quantitative metabolomics with a linear mixed-effect model for analysis of urinary nucleosides and deoxynucleosides from bladder cancer patients before and after tumor resection

Artymowicz, Małgorzata; Struck-Lewicka, Wiktoria; Wiczling, Paweł; Markuszewski, Marcin; Markuszewski, Michał J.; Siluk, Danuta

doi:10.1007/s00216-023-04826-0

Cited by 6 publications

(3 citation statements)

References 52 publications

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“…Targeted metabolomics enables researchers to investigate specific compounds or compound groups within metabolites [36][37][38]. In scenarios with limited known data, untargeted analyses are more appropriate.…”

Section: Targeted and Untargeted Analysismentioning

confidence: 99%

Review on analytical technologies and applications in metabolomics

MENG,

LIU,

et al. 2024

BIOCELL

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Over the past decade, the swift advancement of metabolomics can be credited to significant progress in technologies such as mass spectrometry, nuclear magnetic resonance, and multivariate statistics. Currently, metabolomics garners widespread application across diverse fields including drug research and development, early disease detection, toxicology, food and nutrition science, biology, prescription, and chinmedomics, among others.Metabolomics serves as an effective characterization technique, offering insights into physiological process alterations in vivo. These changes may result from various exogenous factors like environmental conditions, stress, medications, as well as endogenous elements including genetic and protein-based influences. The potential scientific outcomes gleaned from these insights have catalyzed the formulation of innovative methods, poised to further broaden the scope of this domain. Today, metabolomics has evolved into a valuable and widely accepted instrument in the life sciences. However, comprehensive reviews focusing on the sample preparation and analytical methodologies employed in metabolomics within the life sciences are surprisingly scant. This review aims to fill that gap, providing an overview of current trends and recent advancements in metabolomics. Particular emphasis is placed on sample preparation, sophisticated analytical techniques, and their applications in life science research.

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Section: Targeted and Untargeted Analysismentioning

confidence: 99%

Review on analytical technologies and applications in metabolomics

MENG,

LIU,

et al. 2024

BIOCELL

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“…This approach has become standard practice in certain types of studies, such as urine analysis. 39 , 40 , 41 The use of artificial urine is relatively common, and protocols exist to prepare this artificial matrix from a mixture of salts, creatinine, urea, and uric acid 42 , 43 (Figure 1A ). However, this approach requires many preparation steps, and the replicated matrix can still be quite different from the real samples.…”

Section: Analytical Standards For Absolute Quantitationmentioning

confidence: 99%

Challenges and recent advances in quantitative mass spectrometry‐based metabolomics

Ghafari,

Sleno

2024

Analytical Science Advances

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The field of metabolomics has gained tremendous interest in recent years. Whether the goal is to discover biomarkers related to certain pathologies or to better understand the impact of a drug or contaminant, numerous studies have demonstrated how crucial it is to understand variations in metabolism. Detailed knowledge of metabolic variabilities can lead to more effective treatments, as well as faster or less invasive diagnostics. Exploratory approaches are often employed in metabolomics, using relative quantitation to look at perturbations between groups of samples. Most metabolomics studies have been based on metabolite profiling using relative quantitation, with very few studies using an approach for absolute quantitation. Using accurate quantitation facilitates the comparison between different studies, as well as enabling longitudinal studies. In this review, we discuss the most widely used techniques for quantitative metabolomics using mass spectrometry (MS). Various aspects will be addressed, such as the use of external and/or internal standards, derivatization techniques, in vivo isotopic labelling, or quantitative MS imaging. The principles, as well as the associated limitations and challenges, will be described for each approach.

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“…Mass spectrometric approaches (x) monitor continuous flow chemical reactions; (xi) examine complex analyte mixtures in biological tissues and fluids [37,40,59], environmental [60][61][62], and foodstuff samples; (xii) are used for in vivo diagnostics; and (xiii) experimentally determine kinetics, thermodynamics, diffusion, and ion mobility parameters of chemicals and their reactions [63][64][65][66][67][68].…”

mentioning

confidence: 99%