Targeted Proteomic Analyses of Nasal Lavage Fluid in Persulfate-Challenged Hairdressers with Bleaching Powder-Associated Rhinitis

Abstract

Hairdressers have an increased risk for developing airway symptoms, for example, asthma and rhinitis. Persulfates, which are oxidizing agents in bleaching powder, are considered important causal agents for these symptoms. However, the underlying mechanisms are unclear. The aim was therefore to measure proteomic changes in nasal lavage fluid from persulfate-challenged subjects to identify proteins potentially involved in the pathogenesis of bleaching powder-associated rhinitis or candidate effect biomarkers for persulfate. Also, oxidized peptides were measured to evaluate their usefulness as biomarkers for persulfate exposure or effect, for example, oxidative stress. Samples from hairdressers with and without bleaching powder-associated rhinitis were analyzed with liquid chromatography tandem mass spectrometry using selected reaction monitoring to target 246 proteins and five oxidized peptides. Pathway analysis was applied to obtain a functional overview of the proteins. Several proteins involved in biologically meaningful pathways, functions, or disorders, for example, inflammatory responses, oxidative stress, epithelium integrity, and dermatological disorders, changed after the persulfate challenge. A list with nine proteins that appeared to be affected by the persulfate challenge and should be followed up was defined. An albumin peptide containing oxidized tryptophan increased 2 h and 5 h after the challenge but not after 20 min, which indicates that such peptides may be useful as oxidative stress biomarkers.

Nasal protein profiles in work‐related asthma caused by different exposures

“…GSTP1 gene activation is mainly regulated by Nrf2, and the functional association between GSTP and Nrf2 proteins supports the regulatory role of GSTP in the adaption response to cellular stresses produced in the course of inflammatory and oxidative reactions . As in the present study, GSTP was decreased in NLF after the persulphate challenge …”

“…Few studies have reported the proteome of work‐related airway diseases. Mörtstedt et al performed targeted proteomic analyses of NLF before and after a persulfate challenge among hairdressers with bleaching powder‐associated rhinitis as compared to healthy hairdressers and atopic subjects. They detected changes in proteins related to inflammatory responses and oxidative stress, but the changes were not specific to rhinitis patients.…”

Metaproteogenomics Reveals Taxonomic and Functional Changes between Cecal and Fecal Microbiota in Mouse

“…The p -value list provided by the ibb test was subsequently subjected to a multiple testing adjustment based on a sequential goodness of fit (SGoF) metatest (Carvajal-Rodriguez et al, 2009) using the SGoF+ software (v.3.8) with default parameters (Carvajal-Rodriguez and de Una-Alvarez, 2011). This metatest has been successfully applied to large RNA-seq and proteomic datasets (Shi et al, 2012; Mortstedt et al, 2015), and was applied in this study in view of its suitability for small sample size and ability to maintain a high statistical power when increasing the number of test. An adjusted p -value < 0.05 was considered as the threshold for statistical significance of differential results.…”

In-Depth, Proteomic Analysis of Nasal Secretions from Patients With Chronic Rhinosinusitis and Nasal Polyps

“…Nasal samples, including tissues,6 lavages,7 swabs8 and suctions,910 have been used to identify the CRS endotypes. However, the invasive procedures ( e.g., biopsy) are associated with the risk of infection and must be performed by experts 11.…”

“…Nasal lavages, swabs and suctions have alternatively been obtained; these are constantly available, and sampling does not involve a risk of infection 11. Moreover, the proteins in nasal samples like the antimicrobial proteins1213 and immunoglobulins14 could reflect the upper airway diseases 7891015. Thus, the nasal secretions are valuable when studying CRS endotypes 11.…”