“…Mass spectrometry compatible, or acid-labile, detergents may also provide specificity (Ross et al, 2002;Nomura et al, 2004). Another means to avoid excessive background would be to use the affinity receptor immobilized at a high density on a low total surface area with concomitantly less area available for non-specific binding (Weinberger, Morris, & Pawlak, 2000;Weinberger et al, 2002;de Seny et al, 2008), such as a 96-well plate used in ELISA assays (Kulasingam et al, 2008), PVDF MASS SPECTROMETRY OF BLOOD & membrane (Chen et al, 2007b) et al, 2008). Antigen-antibody complexes in the 150-400 kDa range were chemically cross-linked and directly detected by MALDI mass spectrometry (Nazabal, Wenzel, & Zenobi, 2006).…”