Targeted Nuclear Delivery using Peptide-Coated Quantum Dots

Kuo, Chien-Nan; Chueh, Di-Yen; Singh, Narendra Pratap; Chien, Fan‐Ching; Chen, Peilin

doi:10.1021/bc100527m

Cited by 47 publications

(30 citation statements)

References 41 publications

(52 reference statements)

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“…Moreover, they have high resistance to both of photobleaching and chemical degradation. 4,[8][9][10][11] As widely acknowledged, however, the traditional SQDs have a heavy metal core that prevents its application for in vivo assays. [12][13][14] In addition, the uorescence blinking in single SQDs, is harmful for dynamic tracking due to information loss.…”

Section: Introductionmentioning

confidence: 99%

Cyto-toxicity, biocompatibility and cellular response of carbon dots–plasmonic based nano-hybrids for bioimaging

et al. 2017

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In this study, hybrid carbon dots-plasmonic nanostructures including carbon dots/polyethyleneimine/gold (C-dots/PEI/Au), and carbon dots/polyethyleneimine/silver (C-dots/PEI/Ag) have been prepared using a microwave irradiation method. The prepared hybrid nanostructures have been characterized via optical spectroscopy, high resolution transmission electron microscopy (HRTEM), and X-ray diffraction (XRD).A remarkable enhancement in the optical parameters, such as absorptivity and quantum yield (QY), has been observed for the hybrid nanostructure compared to pure carbon dots. This plasmonic enhancement was more pronounced in the presence of silver (C-dots/PEI/Ag nanohybrid) than that of gold (C-dots/PEI/Au nanohybrid). This is referred to the low intrinsic loss and the degree of the overlap between the absorption spectra of silver nanoparticles and carbon dots. Furthermore, the biocompatibility assay and cellular response on epithelial kidney (Vero) normal cell has been investigated.The results showed that the optimal dose of treatment is about $200 mg ml À1 using both C-dots/PEI/Au or C-dots/PEI/Ag, nano-hybrids could be used safely in diagnostic bioimaging applications.

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Section: Introductionmentioning

confidence: 99%

Cyto-toxicity, biocompatibility and cellular response of carbon dots–plasmonic based nano-hybrids for bioimaging

et al. 2017

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“…Equipping QDs with cysteine-terminated NLS peptides, such as the adenovirus-derived CGGFSTSLRARKA, the SV40 large T antigen-derived CGGGPKKKRKVGG or the HIV-1 Tat protein-derived CGGRKKRRQRRRAP by means of the heterobifunctional linker (Succinimidyl-4-[N-maleimidomethyl] cyclohexane-1-carboxylate) led to efficient cellular internalization. This modification also allowed active targeting of the nucleus in spite of the increased size of the particles (14.1-18 nm in diameter) [36]. We should mention that the cellular uptake can be influenced not only by the conjugation of CPPs, but also by the number of QD-associated CPPs and the cell-type, which has been confirmed by Delehanty et al [88] The functionalization of QDs with CPPs can facilitate their transport across the plasma membrane, and thus enable these particles to cross the blood-brain barrier (BBB).…”

Section: Delivery Of Nanoparticles Using Cpps Quantum Dotsmentioning

confidence: 99%

Cell-penetrating peptides for nanomedicine – how to choose the right peptide

2015

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More than two decades ago, a group of peptides, now known as cell-penetrating peptides, sparked the hope that the ultimate carrier molecules have been found. The high expectations for these peptides, which are reflected in their bold name, led to many disappointments due to the controversial results their utilization entailed and nowadays even their effectiveness has been called into question. In this review, we discuss the uptake mechanism and application of cell penetrating peptides as mediators for organelle specific delivery of nanocarriers, pointing out the possibilities as well as strategies of their successful utilization. Additionally, we provide an overview of the conjugation techniques usually employed for the attachment of cell penetrating peptides to quantum dots, as well as silver and gold nanoparticles, and we address the various aspects that need to be considered for the successful implementation of cell penetrating peptides for organelle-specific delivery of nanoparticles into cells.Keywords: cell penetrating peptide; drug delivery; gold nanoparticle; nanomedicine; polymeric nanoparticle; quantum dot. Highlights-We discuss the mechanisms of cell penetrating peptide's uptake and briefly introduce the different types of peptides in order to present a more complete picture of the intracellular fate of the peptide-cargo conjugates. -We highlight the recent applications of cell penetrating peptides in the targeted delivery of quantum dots as well as gold, silver and polymeric nanoparticles into mammalian cells in the context of organelle specificity. -We give a short overview of the conjugation techniques usually utilized for the attachment of cell penetrating peptides to quantum dots as well as silver and gold nanoparticles.

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“…The biocomposites formation via biofunctionalization of QDs in conjunction with proteins, DNA, peptides, enzymes, polymers etc., has evolved over the past decade and is thus of growing interest and innovation. [7][8][9][10] Efforts are also being made for applications of QDs in live cells, in vivo imaging, labeling, sensing of biological and as environmental elements.…”

Section: Introductionmentioning

confidence: 99%