Targeted mutation of barley (1,3;1,4)‐β‐glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content

Garcia-Gimenez, Guillermo; Barakate, Abdellah; Smith, Pauline; Stephens, Jennifer; Khor, Shi F.; Doblin, Monika S.; Hao, Pengfei; Bacic, Antony; Burton, Rachel A.; Waugh, Robbie; Tucker, Matthew R.; Houston, Kelly

doi:10.1111/tpj.14977

Cited by 38 publications

(27 citation statements)

References 68 publications

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“…2c,f). Field-testing for agronomic performance of cslF6 W676* confirmed previously published greenhouse experiments on CslF6 knockouts, namely that TGW and grain width were reduced and changed, respectively 21 (Fig. 2d-f; Fig.…”

Section: Resultssupporting

confidence: 82%

FIND-IT: Ultrafast mining of genome diversity

Knudsen

Wendt

Dockter

et al. 2021

Preprint

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Novel crop improvement methodologies, including the exploitation of natural genetic variation, are urgently required to feed our rapidly growing human population in the context of global climate change. Here we describe a ‘Fast Identification of Nucleotide variants by DigITal PCR’ (FIND-IT) method for the rapid identification of pre-targeted genetic variants or rare alleles in large genomic populations. Libraries of 500,000 individuals can be screened and desired variants isolated within two weeks. FIND-IT is widely applicable for mining valuable diversity in any genomic population, including elite breeding and wild germplasm collections. The method provides single nucleotide resolution that has been validated by identifying and isolating knockout lines, non-synonymous codon changes and variants of miRNA and transcription factor binding sites in the agronomically important crop barley. In contrast to existing methods, FIND-IT does not require transformation, cloning or enzymatic steps, and is exempt from GMO regulations. Thus, FIND-IT can be applied immediately to elite crop cultivars and can be tailored to minimize or eliminate time-consuming backcrossing requirements.

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Section: Resultssupporting

confidence: 82%

FIND-IT: Ultrafast mining of genome diversity

Knudsen

Wendt

Dockter

et al. 2021

Preprint

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“…In this scenario, selection at QRMC-3HS may contribute to the fine-tuning of these multitrophic interactions. Recent innovations in barley genetics [72][73][74][75] will facilitate the development of refined genetic material required to probe these scenarios experimentally.…”

Section: Discussionmentioning

confidence: 99%

Identifying Plant Genes Shaping Microbiota Composition in the Barley Rhizosphere

Escudero-Martinez

Coulter

Terrazas

et al. 2021

Preprint

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A prerequisite to exploiting soil microbes for sustainable crop production is the identification of the plant genes shaping microbiota composition in the rhizosphere, the interface between roots and soil. Here we used metagenomics information as an external quantitative phenotype to map the host genetic determinants of the rhizosphere microbiota in wild and domesticated genotypes of barley, the fourth most cultivated cereal globally. We identified a small number of loci with a major effect on the composition of rhizosphere communities. One of those, designated the QRMC-3HS locus, emerged as a major determinant of microbiota composition. We then subjected soil-grown sibling lines harbouring contrasting alleles at QRMC-3HS and hosting contrasting microbiotas to comparative root RNA-seq profiling. This allowed us to identify three primary candidate genes, including a Nucleotide-Binding-Leucine-Rich-Repeat (NLR) gene in a region of structural variation of the barley genome. Our results provide novel insights into the footprint of crop improvement on the plants capacity of shaping rhizosphere microbes.

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“…Another trait that is favored for brewing is low (1,3;1,4)-b-glucan content. To investigate enzymes contributing to high grain b-glucan content, two known and two putative (1,3;1,4)-b-glucan synthases were knocked out independently (Garcia-Gimenez et al 2020). The authors could confirm the major role of HvCslF6 in grain b-glucan production.…”

Section: Barleymentioning

confidence: 99%

Genome editing in cereal crops: an overview

et al. 2021

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Genome-editing technologies offer unprecedented opportunities for crop improvement with superior precision and speed. This review presents an analysis of the current state of genome editing in the major cereal crops- rice, maize, wheat and barley. Genome editing has been used to achieve important agronomic and quality traits in cereals. These include adaptive traits to mitigate the effects of climate change, tolerance to biotic stresses, higher yields, more optimal plant architecture, improved grain quality and nutritional content, and safer products. Not all traits can be achieved through genome editing, and several technical and regulatory challenges need to be overcome for the technology to realize its full potential. Genome editing, however, has already revolutionized cereal crop improvement and is poised to shape future agricultural practices in conjunction with other breeding innovations.

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Targeted mutation of barley (1,3;1,4)‐β‐glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content

Cited by 38 publications

References 68 publications

FIND-IT: Ultrafast mining of genome diversity

FIND-IT: Ultrafast mining of genome diversity

Identifying Plant Genes Shaping Microbiota Composition in the Barley Rhizosphere

Genome editing in cereal crops: an overview

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