Targeted Mutation of a Non-catalytic Gating Residue Increases the Rate of Pseudomonas aeruginosad-Arginine Dehydrogenase Catalytic Turnover
Joanna Afokai Quaye,
Daniel Ouedraogo,
Giovanni Gadda
Abstract:Commercial food and l-amino acid industries
rely on bioengineered d-amino acid oxidizing enzymes to detect
and remove d-amino acid contaminants. However, the bioengineering
of enzymes
to generate faster biological catalysts has proven difficult as a
result of the failure to target specific kinetic steps that limit
enzyme turnover, k
cat, and the poor understanding
of loop dynamics critical for catalysis. Pseudomonas
aeruginosa
d-arginine dehydrogenase (PaDADH) oxidizes most d-amino acids and is a good
candidat… Show more
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