Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System

Genome Editing with Engineered Nucleases in Economically Important Animals and Plants: State of the Art in the Research Pipeline

“…The phosphorus metabolism in both plants and animals is quickly becoming an important issue. Moreover, increased phosphorus concentration in surface waters from surface runoff or manure has detrimental effects on aquatic ecosystems (Shukla et al, 2009;Liang et al, 2014 The knock-in of the pat gene thus effectively and hereditarily mutated the ipk1 and, moreover, simultaneously conferred herbicide resistance. Furthermore, no off-target effects were observed.…”

“…Furthermore, no off-target effects were observed. Liang et al (2014) have also successfully introduced small deletions and insertions into the ipk gene using TALENs and CRISPR/Cas9 that proved to be heritable in the case of the TALENs-mediated mutation.…”

Genome engineering of woody plants: past, present and future

“…It is interesting to note that liverwort is amenable to genome editing using haploid generation to obtain knockout plants in the parent generation of gene transfer (T0 generation). Other than model plants, genome editing with the CRISPR/Cas9 system has been performed in many crop plants, e.g., maize [57], rice [54,58], sorghum [59], soybean [60,61], tomato [62], and wheat [54,63]. These reports demonstrated (1) a high-frequency of mutation following gene transfer of sgRNA/Cas9 via Agrobacterium-mediated transformation, with a variety of mutation rates depending on species; in rice, mutation efficiency reached 100 % [58], (2) the achievement of biallelic mutations after gene transfer of sgRNA/Cas9 in the T0 generation in several crop plants [58,[60][61][62], and (3) that multiplex gRNA expression can lead to simultaneous mutation of multiple target genes [54,58,[60][61][62][63].…”

7. Themenbereich grüne Gentechnologie: Pflanzenzüchtung und Agrarwirtschaft

“…Dies schließt den erfolgreichen Einsatz der Technologie in unterschiedlichen Modell-und Nutzpflanzen, wie beispielsweise Ackerschmalwand (Feng et al, 2014), Reis (Xu et al, 2014), Mais (Liang et al, 2014) und Orange (Jia und Wang, 2014) ein. Aktuelle Übersichten zu den Möglichkeiten der CRISPR-Technologie und ihrer zukünftigen Verwendung in der Pflanzenzüchtung finden sich bei Liu und Fan (2014) sowie Lozano-Juste und Cutler (2014).…”