Targeted Metabolomics of Dried Blood Spot Extracts

Zukunft, Sven; Sorgenfrei, Martina; Prehn, Cornelia; Möller, Gabriele; Adamski, Jerzy

doi:10.1007/s10337-013-2429-3

Cited by 97 publications

(84 citation statements)

References 26 publications

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“…Moreover, extraction protocols are sample dependent [29,30], and specific extraction procedures are recommended for cells [31], serum [18], urine [32], and dried blood spots [15,33], respectively.…”

Section: Europe Pmc Funders Author Manuscriptsmentioning

confidence: 99%

“…Nevertheless, another study integrating targeted metabolomics with DBS collection shows that some metabolites were stable for 4 weeks, even at room temperature, stored in sealed bags with desiccant packages [33].…”

Section: Evaluation Of the Metabolome Stabilitymentioning

confidence: 99%

“…The stability seems to be both chemical class and metabolite dependent; for instance, Zukunft et al showed that glutamine and histidine stored at room temperature in sealed bags with desiccants are degraded after 2 weeks, while other amino acids such as asparagine were stable for weeks [33]. On the other hand, the same paper showed that lipids such as …”

Section: Evaluation Of the Metabolome Stabilitymentioning

confidence: 99%

“…Europe PMC Funders Author Manuscripts phosphatidylcholines and sphingomyelins are unstable at room temperature stored in sealed bags (in all samples or after hours/days/weeks) [33].…”

Section: Europe Pmc Funders Author Manuscriptsmentioning

confidence: 99%

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Pre-analytic evaluation of volumetric absorptive microsampling and integration in a mass spectrometry-based metabolomics workflow

et al. 2017

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Volumetric absorptive microsampling (VAMS) is a novel approach that allows single-drop (10 μL) blood collection. Integration of VAMS with mass spectrometry (MS)-based untargeted metabolomics is an attractive solution for both human and animal studies. However, to boost the use of VAMS in metabolomics, key pre-analytical questions need to be addressed. Therefore, in this work, we integrated VAMS in a MS-based untargeted metabolomics workflow and investigated pre-analytical strategies such as sample extraction procedures and metabolome stability at different storage conditions. We first evaluated the best extraction procedure for the polar metabolome and found that the highest number and amount of metabolites were recovered upon extraction with acetonitrile/water (70:30). In contrast, basic conditions (pH 9) resulted in divergent metabolite profiles mainly resulting from the extraction of intracellular metabolites originating from red blood cells. In addition, the prolonged storage of blood samples at room temperature caused significant changes in metabolome composition, but once the VAMS devices were stored at − 80 °C, the metabolome remained stable for up to 6 months. The time used for drying the sample did also affect the metabolome. In fact, some metabolites were rapidly degraded or accumulated in the sample during the first 48 h at room temperature, indicating that a longer drying step will significantly change the concentration in the sample. KeywordsMetabolomics; Volumetric absorptive microsampling; Mass spectrometry ✉ Giuseppe Paglia beppepaglia@gmail.com. Compliance with ethical standardsThis study was performed in accordance with the ethical standards. The local ethics committee (Comitato etico del comprensorio sanitario di Bolzano) approved the study, and all participants provided written informed consent.

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Section: Europe Pmc Funders Author Manuscriptsmentioning

confidence: 99%

Section: Evaluation Of the Metabolome Stabilitymentioning

confidence: 99%

Section: Evaluation Of the Metabolome Stabilitymentioning

confidence: 99%

“…Europe PMC Funders Author Manuscripts phosphatidylcholines and sphingomyelins are unstable at room temperature stored in sealed bags (in all samples or after hours/days/weeks) [33].…”

Section: Europe Pmc Funders Author Manuscriptsmentioning

confidence: 99%

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Pre-analytic evaluation of volumetric absorptive microsampling and integration in a mass spectrometry-based metabolomics workflow

et al. 2017

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“…This assay can simultaneously quantify 188 metabolites in 10 μl plasma, including 40 acylcarnitines (Cx:y, where x indicates the total number of carbons and y the number of double bonds in the fatty acid side chains), 21 amino acids (19 proteinogenic plus citrulline and ornithine), 21 biogenic amines, total hexoses, 90 glycerophospholipids (14 lysophosphatidylcholines [lysoPCs] and 76 phosphatidylcholines [PCs]), and 15 sphingolipids. All analytical procedures were performed as previously described [21,22]. Briefly, 10 μl plasma was pipetted onto a filter which is incorporated into a 96-well sandwich plate and already contains internal standards stable isotope labelled with stable isotope.…”

Section: Measurement Of Plasma Metabolitesmentioning

confidence: 99%

Lactation is associated with altered metabolomic signatures in women with gestational diabetes

et al. 2016

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Aims/hypothesis Lactation for >3 months in women with gestational diabetes is associated with a reduced risk of type 2 diabetes that persists for up to 15 years postpartum. However, the underlying mechanisms are unknown. We examined whether in women with gestational diabetes lactation for >3 months is associated with altered metabolomic signatures postpartum. Methods We enrolled 197 women with gestational diabetes at a median of 3.6 years (interquartile range 0.7-6.5 years) after delivery. Targeted metabolomics profiles (including 156 metabolites) were obtained during a glucose challenge test. Comparisons of metabolite concentrations and ratios between women who lactated for >3 months and women who lactated for ≤3 months or not at all were performed using linear regression with adjustment for age and BMI at the postpartum visit, time since delivery, and maternal education level, and correction for multiple testing. Gaussian graphical modelling was used to generate metabolite networks.Results Lactation for >3 months was associated with a higher total lysophosphatidylcholine/total phosphatidylcholine ratio; in women with short-term follow-up, it was also associated with lower leucine concentrations and a lower total branchedchain amino acid concentration. Gaussian graphical modelling identified subgroups of closely linked metabolites within phosphatidylcholines and branched-chain amino acids that were affected by lactation for >3 months and have been linked to the pathophysiology of type 2 diabetes in previous studies. Conclusions/interpretation Lactation for >3 months in women with gestational diabetes is associated with changes in the metabolomics profile that have been linked to the early pathogenesis of type 2 diabetes.

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Current trends in quantitative proteomics – an update

Han

Pan

et al. 2017

J. Mass Spectrom.

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Proteins can provide insights into biological processes at the functional level, so they are very promising biomarker candidates. The quantification of proteins in biological samples has been routinely used for the diagnosis of diseases and monitoring the treatment. Although large-scale protein quantification in complex samples is still a challenging task, a great amount of effort has been made to advance the technologies that enable quantitative proteomics. Seven years ago, in 2009, we wrote an article about the current trends in quantitative proteomics. In writing this current paper, we realized that, today, we have an even wider selection of potential tools for quantitative proteomics. These tools include new derivatization reagents, novel sampling formats, new types of analyzers and scanning techniques, and recently developed software to assist in assay development and data analysis. In this review article, we will discuss these innovative methods, and their current and potential applications in proteomics. Copyright © 2017 John Wiley & Sons, Ltd.

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Targeted Metabolomics of Dried Blood Spot Extracts

Cited by 97 publications

References 26 publications

Pre-analytic evaluation of volumetric absorptive microsampling and integration in a mass spectrometry-based metabolomics workflow

Pre-analytic evaluation of volumetric absorptive microsampling and integration in a mass spectrometry-based metabolomics workflow

Lactation is associated with altered metabolomic signatures in women with gestational diabetes

Current trends in quantitative proteomics – an update

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