Targeted metabolomic profiling for acute myocardial infarction pathogenesis

Abstract

Background. Acute myocardial infarction (AMI) represents a major cause of morbidity and mortality worldwide. Metabolomics investigation may be useful in the AMI pathogenesis research. Materials and methods. The article describes a comprehensive study of the AMI through the targeted metabolomic profiling. A total of 195 subjects were enrolled in the study, consisting of 68 AMI patients, 84 IHD patients and 43 non-CVD subjects. Metabolomic profiling was conducted, involving the quantitative analysis of 87 endogenous metabolites in plasma. Results. We identified 36 significantly changed metabolites in AMI, which included increased cystathionine and dimethylglycine and the decreased asymmetric dimethylarginine (ADMA) and arginine. It was found, that patients with AMI had significantly lower concentration of short chain acylcarnitines as compared to IHD and non-CVD patient. In patients with AMI concentration of xanthurenic acid and 3-OH-kynurenine was significantly decreased, as compared to IHD patients and non-CVD subjects. Norepinephrine was significantly decreased in patients with AMI and IHD, whereas its end-product – vanillylmandelic acid (VMA) – significantly increased. Based on the differences in the constructed weighted correlation networks, there were found new significant ratios of the metabolites. Among 23 established significantly altered metabolite ratios 14 ratios between non-CVD vs AMI and 17 ratios between IHD vs AMI were found. 9 ratios between non-CVD vs AMI and IHD vs AMI and 2 ratios between non-CVD vs IHD vs AMI were coincided. Conclusion. Obtained findings may pave the way for new insight of АMI pathogenesis and ultimately improving clinical outcomes.