Targeted Metabolic and Genomic Profiling Reveals Parents for L-Dopa Breeding in Mucuna pruriens (L.) DC.

Leelambika, M.; Mahesh, S.; Jaheer, Mahammad; Tripathi, Pankaj Kumar; Kumar, Pittala Ranjith; Sathyanarayana, N.

doi:10.1007/s12042-016-9174-3

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…Results demonstrated small correlation (R = 0.122). In several studies, no correlation was found between genetic analysis and metabolomic profile [ 1 , 2 , 56 ]. This can be attributed to the fact that markers, such as ScoT markers, cannot unravel differences in gene function that can cause variations in metabolic profiles.…”

Section: Discussionmentioning

confidence: 99%

“…Phenotypic traits and DNA markers, which represent various aspects of genome polymorphism and are occasionally used independently in parental selection, are the two main data sources. However, integration of phenotypic data with genotypic information is highly advised when neutral DNA markers are used for this purpose [ 1 , 2 ]. This aspect becomes even more important when it comes to biochemical characteristics, whose expression is constrained, among others, by the fitness they provide to plants.…”

Section: Introductionmentioning

confidence: 99%

“…This aspect becomes even more important when it comes to biochemical characteristics, whose expression is constrained, among others, by the fitness they provide to plants. Combining DNA markers with chemical profiling is thought to be required in such cases, especially for crops with a long history of domestication that include chemical phenotypes commercially relevant [ 1 ]. Since noncoding sequences comprise the majority of plant genomes, most changes in molecular marker patterns are predicted to be caused by neutral mutations that are fixed by genetic drift rather than by selection [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

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Genetic and Metabolite Variability among Commercial Varieties and Advanced Lines of Vicia faba L.

Avramidou

Sarri

Ganopoulos

et al. 2023

Plants

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Vicia faba L. (faba bean) is one of the most promising pulse crops due to its nutritional value and high nitrogen fixation capacity. The aim of the present study was to compare the genetic diversity and the seed metabolite profiles of five genetic materials of faba bean. Specifically, three newly developed advanced lines (KK18, KK14 and KK10) and two commercial cultivars (POLIKARPI and TANAGRA), were evaluated for this purpose. Genetic diversity among populations was assessed by SCoT molecular markers. Through UPGMA dendrogram, genetic distances between populations were estimated. Untargeted metabolomics analysis of the seeds was performed employing GC/EI/MS. The cultivar POLYKARPI exhibited the highest polymorphism. All varieties showed a higher within-cultivars and advanced lines variability than between. POLYKARPI and KK14 had the lowest genetic distances, while KK18 and TANAGRA presented the highest ones. The advanced line KK18 displayed the best nutritional profile, the highest concentration of desirable metabolites (lactic acid and trehalose), the lowest concentration of anti-nutritional factors (oxalic acid) and the lowest concentration of saturated fatty acids (palmitic and stearic acid). According to the results of the present study, KK18 line is a very promising material for further exploration and utilization in breeding programs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic and Metabolite Variability among Commercial Varieties and Advanced Lines of Vicia faba L.

Avramidou

Sarri

Ganopoulos

et al. 2023

Plants

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Genetic diversity and metabolic profile of Salvia officinalis populations: implications for advanced breeding strategies

Sarrou

Ganopoulos

Xanthopoulou

et al. 2017

Planta

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As a result of this work, we were able to characterize seven indigenous to Greece Salvia officinalis populations using genetic and metabolomic tools. These tools can be used to select the most promising genotypes, capable to design future breeding programs for high valuable varieties. An initial investigation was carried out to compare the genetic and metabolic diversity in S. officinalis grown in Greece and to discern the relationship between the two sets of data. Analysis of inter-simple sequence repeats (ISSR) revealed significant genetic differences among seven sage populations, which were grouped into three main clusters according to an UPGMA ISSR data-based dendrogram and Principle Coordinate Analysis. 80 loci were scored of which up to 90% were polymorphic at species level. According to the composition of their essential oil, the populations were classified into two chemotypes: 1.8 cineole/α-thujone and α-thujone/1.8 cineole. Additionally, a targeted ultra performance liquid chromatography (UPLC-MS/MS) method was used to qualify and quantify phenolic compounds in methanolic extracts of the seven sage genotypes according to which they were districted in six clusters among the sage populations. The main compounds characterizing the seven genotypes were rosmarinic acid and carnosol, followed by apigenin-7-O-glucoside (Ap7glc), and luteolin-7-O-glucoside (Lu7glc). The correlation between matrices obtained from ISSR data and metabolic profiles was non-significant. However, based on the differences in metabolic fingerprint, we aimed to define populations using as main selection criteria the high polyphenol content and desired essential oil composition, using state to the art analytical tools for the identification of parent lines for breeding programs.

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Validation of DNA barcoding markers in common Mucuna species of India for taxonomy and pharmacognosy applications

Rashmi¹,

Sathyanarayana

Vidya³

2017

Plant Gene

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Targeted Metabolic and Genomic Profiling Reveals Parents for L-Dopa Breeding in Mucuna pruriens (L.) DC.

Cited by 5 publications

References 26 publications

Genetic and Metabolite Variability among Commercial Varieties and Advanced Lines of Vicia faba L.

Genetic and Metabolite Variability among Commercial Varieties and Advanced Lines of Vicia faba L.

Genetic diversity and metabolic profile of Salvia officinalis populations: implications for advanced breeding strategies

Validation of DNA barcoding markers in common Mucuna species of India for taxonomy and pharmacognosy applications

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