Targeted knockout of duox causes defects in zebrafish growth, thyroid development, and social interaction

Park, Jong‐Su; Choi, Tae‐Ik; Kim, Oc-Hee; Hong, Ted; Kim, Woo‐Keun; Lee, Won–Jae; Kim, Cheol-Hee

doi:10.1016/j.jgg.2019.01.004

Cited by 8 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4B and SI Appendix, Table S6). Within the differentially expressed genes, we observed duox, which encodes a dual oxidase implicated in TH production (27,35). This gene was significantly overexpressed in new recruits from S. gigantea (adjusted P = 0.038, Log 2 FC =2.53) compared to those from H. magnifica.…”

Section: Resultsmentioning

confidence: 94%

“…For this, we used zebrafish Danio rerio, in which iridophores depend on TH for their maturation (22). duox requirements have been described for somatic development and melanophore numbers but not iridophore pattern (27,35). We therefore injected one-cell stage embryos of the iridophore reporter line Tg(pnp4a:palm-mcherry) wprt10Tg with highly efficient Alt-R CRISPR-Cas9 (36) targeting duox, resulting in phenotypes concordant with those for this locus (27,35) and other hypothyroid fish (21,22).…”

Section: Resultsmentioning

confidence: 99%

“…duox requirements have been described for somatic development and melanophore numbers but not iridophore pattern (27,35). We therefore injected one-cell stage embryos of the iridophore reporter line Tg(pnp4a:palm-mcherry) wprt10Tg with highly efficient Alt-R CRISPR-Cas9 (36) targeting duox, resulting in phenotypes concordant with those for this locus (27,35) and other hypothyroid fish (21,22). started to form ventrally; some loosely arranged iridophores occur in between, where a melanophore stripe develops (37).…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Thyroid hormones regulate the formation and environmental plasticity of white bars in clownfishes

Salis

Roux

Huang

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Determining how plasticity of developmental traits responds to environmental conditions is a challenge that must combine evolutionary sciences, ecology, and developmental biology. During metamorphosis, fish alter their morphology and color pattern according to environmental cues. We observed that juvenile clownfish (Amphiprion percula) modulate the developmental timing of their adult white bar formation during metamorphosis depending on the sea anemone species in which they are recruited. We observed an earlier formation of white bars when clownfish developed with Stichodactyla gigantea (Sg) than with Heteractis magnifica (Hm). As these bars, composed of iridophores, form during metamorphosis, we hypothesized that timing of their development may be thyroid hormone (TH) dependent. We treated clownfish larvae with TH and found that white bars developed earlier than in control fish. We further observed higher TH levels, associated with rapid white bar formation, in juveniles recruited in Sg than in Hm, explaining the faster white bar formation. Transcriptomic analysis of Sg recruits revealed higher expression of duox, a dual oxidase implicated in TH production as compared to Hm recruits. Finally, we showed that duox is an essential regulator of iridophore pattern timing in zebrafish. Taken together, our results suggest that TH controls the timing of adult color pattern formation and that shifts in duox expression and TH levels are associated with ecological differences resulting in divergent ontogenetic trajectories in color pattern development.

show abstract

Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Thyroid hormones regulate the formation and environmental plasticity of white bars in clownfishes

Salis

Roux

Huang

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…About 105 DUOX2 variants have been reported in more than 200 unrelated CH patients (Muzza and Fugazzola, 2017). The last two years witnessed the characterization of several loss-of-function models for duox and duoxa in zebrafish including the present study (Trubiroha et al, 2018;Chopra et al, 2019;Park et al, 2019). The mutation spectrum includes single nucleotide variants as well as small deletions (frameshift as well as in-frame).…”

Section: Discussionmentioning

confidence: 80%

“…During the completion of this manuscript, two studies have reported the phenotypic consequences of duox genetic invalidation in adult zebrafish. The exon 3 of the duox gene was targeted by CRISPR/Cas9 to establish an 8 nucleotide deletion mutant fish line (Park et al, 2019). Fifty percent of adult fish carrying the biallelic duox deletion developed an externally visible goiter highlighting again the permanent goitrogenic effects of Duox inactivation.…”

Section: Discussionmentioning

confidence: 99%