Targeted knockout of a chemokine-like gene increases anxiety and fear responses

Choi, Jung Hwa; Jeong, Yun Mi; Kim, Sujin; Lee, Bo-Young; Ariyasiri, Krishan; Kim, Hyun Taek; Jung, Seung Hyun; Hwang, Kyu Seok; Choi, Tae Ik; Park, Chul O.; Huh, Won Ki; Carl, Michael; Rosenfeld, Jill A.; Raskin, Salmo; Ma, Alan; Gécz, Jozef; Kim, Hyung Goo; Shin, Hyungcheol; Park, Doo Sang; Jamieson, Bradley B.; Kim, Joon S.; Iremonger, Karl J.; Lee, Sang H.; Shin, Hee Sup; Kim, Cheol-Hee

doi:10.1073/pnas.1707663115

Cited by 41 publications

(42 citation statements)

References 59 publications

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“…Zebrafish is a shoaling species, and, in nature, CAS is expected to function in shoals. As such, increased shoaling responses are consistently observed during CAS exposure (Table 1; Speedie & Gerlai, 2008;Lima et al, 2016;Canzian et al, 2017;Choi et al, 2017).…”

Section: /41mentioning

confidence: 90%

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Sensory Ecology of Ostariophysan Alarm Substances

Maximino¹,

Silva²,

Campos³

et al. 2018

Preprint

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Chemical communication of predation risk has evolved multiple times in fish species, with the conspecific alarm substance (CAS) contemporaneously being the most well understood mechanism. CAS is released after epithelial damage, usually when prey fish is captured by a predator, and elicits neurobehavioral adjustments in conspecifics which increase the probability of avoiding predation. As such, CAS is a partial predator stimulus, eliciting risk assessment-like and avoidance behaviors, and disrupting the predator sequence. The present paper reviews the distribution and putative composition of CAS in fish, and presents a model for the neural processing of these structures by the olfactory and the brain aversive systems. Applications of CAS in the behavioral neurosciences and neuropharmacology are also presented, exploiting the potential of model fish (e.g., zebrafish, guppies, minnows) on neurobehavioral research.

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Section: /41mentioning

confidence: 90%

“…Shoaling Zebrafish ↑ (Speedie & Gerlai, 2008;Lima et al, 2016;Canzian et al, 2017;Choi et al, 2017) Fathead minnows…”

Section: Not Testedmentioning

confidence: 99%

Sensory Ecology of Ostariophysan Alarm Substances

Maximino¹,

Silva²,

Campos³

et al. 2018

Preprint

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show abstract

“…[62,63]. These tests have also been used to study the neural mechanisms of anxiety-like behavior [64][65][66][67][68]. Thus, these tests can be used both as screening tests and as behavioral bioassays.…”

Section: Behavioral Models In Zebra Fishmentioning

confidence: 99%

New Developments in Behavioral Pharmacology

Cueto‐Escobedo¹,

García-García²,

Maximino³

et al. 2020

Behavioral Pharmacology - From Basic to Clinical Research

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Behavioral pharmacology research has been a cornerstone in the understanding of the processes that underlie the behavior of living organisms as well as the biological basis of the behavioral, emotional, and cognitive disorders that affect humans. The findings in this area have helped to explore the potential therapeutic effects of several substances for the treatment of the mentioned disorders. The present chapter brings an extremely brief introduction to this vast area. First, we try to put in context behavioral pharmacology and its relevance and then show some brief examples of how this discipline has developed over the years. Second, we review the concept of a "research model" in preclinical behavioral pharmacology, given the importance of animal models and tests in this area, followed by a brief review of the recent advances using zebra fish as a valuable tool of research. Third, more specific examples are aborded, such as the findings on sleep disorders and those related to sexual hormones and menopause.

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“…Interestingly, among these differentially expressed genes, we found that chemokine receptors, including cxcr3.2 and cxcr4b, were significantly uprelugated in olfactory mucosae. Recent study has found that the chemokine-like gene family, samdori (sam), especially gene sam2, plays an important role in fear response in zebrafish (Choi et al, 2018). Another important gene, v2rl1, which is a ubiquitously expressed receptor in vertebrate olfactory system (DeMaria et al, 2013;Wakisaka et al, 2017), showed significant downregulation after fright reaction.…”

Section: Broad-scale Differences Of Gene Expression In Zebrafish Aftementioning

confidence: 99%

Comparative Genomics Reveals Accelerated Evolution of Fright Reaction Genes in Ostariophysan Fishes

et al. 2019

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The ostariophysian fishes are the most species-rich clade in freshwaters. This diversification has been suggested to be associated with the fright reaction presented in most ostariophysians. However, the genetic forces that underlie fright reaction remains poorly understood. In the present study, through integrating behavioral, physiological, transcriptomic, and evolutionary genomic analyses, we found that the fright reaction has a broad impact on zebrafish at multiple levels, including changes in swimming behaviors, cortisol levels, and gene expression patterns. In total, 1,555 and 1,599 differentially expressed genes were identified in olfactory mucosae and brain of zebrafish, respectively, with a greater number upregulated after the fright reaction. Functional annotation showed that response to stress and signal transduction were strongly represented, which is directly associated with the fright reaction. These differentially expressed genes were shown to be evolved accelerated under the influence of positive selection, indicating that protein-coding evolution has played a major role in fright reaction. We found the basal vomeronasal type 2 receptors (v2r) gene, v2rl1, displayed significantly decrease expression after fright reaction, which suggests that v2rs may be important to detect the alarm substance and induce the fright reaction. Collectively, based on our transcriptome and evolutionary genomics analyses, we suggest that transcriptional plasticity of gene may play an important role in fright reaction in ostariophysian fishes.

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Targeted knockout of a chemokine-like gene increases anxiety and fear responses

Cited by 41 publications

References 59 publications

Sensory Ecology of Ostariophysan Alarm Substances

Sensory Ecology of Ostariophysan Alarm Substances

New Developments in Behavioral Pharmacology

Comparative Genomics Reveals Accelerated Evolution of Fright Reaction Genes in Ostariophysan Fishes

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