Targeted knock down of CCL22 and CCL17 by siRNA during DC differentiation and maturation affects the recruitment of T subsets

Kang, Shijun; Xie, Jianmin; Ma, Shudong; Liao, Wangjun; Zhang, Junyi; Luo, Rong

doi:10.1016/j.imbio.2009.03.001

Cited by 34 publications

(23 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Expression of CCR4 and its ligands, CCL17 and CCL22, was found in Ti-BALT at the mRNA and protein levels. The CCL17 and CCL22 chemokines have been shown to recruit effector T cells (19)(20)(21)(22), as well as regulatory T cells (Treg; refs. [22][23][24][25] in cancer mouse models.…”

Section: Discussionmentioning

confidence: 99%

“…The CCL17 and CCL22 chemokines have been shown to recruit effector T cells (19)(20)(21)(22), as well as regulatory T cells (Treg; refs. [22][23][24][25] in cancer mouse models. The ambivalent role of these chemokines is confirmed by the fact that CCL22 has been associated with tumor regression in mouse models (20,21,26) and good prognosis in human lung cancer (27), whereas its expression correlates with Treg infiltration and poor prognosis in breast cancer (23).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Chemokines and Adhesion Molecules Associated with T cell Presence in Tertiary Lymphoid Structures in Human Lung Cancer

et al. 2011

View full text Add to dashboard Cite

De novo formation of tertiary lymphoid structures (TLS) has been described in lung cancers. Intratumoral TLS seem to be functional and are associated with a long-term survival for lung cancer patients, suggesting that they represent an activation site for tumor-specific T cells. Here, we characterized T-cell recruitment to TLS in human lung cancer to identify the adhesion molecules and chemoattractants orchestrating this migration. We found that most TLS T cells were CD62Lþ and mainly of CD4þ memory phenotype, but naive T cells were highly enriched in these structures as compared with the rest of the tumor. A specific gene expression signature associated with T cell presence was identified in TLS, which included chemokines (CCL19, CCL21, CXCL13, CCL17, CCL22, and IL16), adhesion molecules (ICAM-2, ICAM-3, VCAM-1, and MAdCAM-1) and integrins (alphaL, alpha4, and alphaD). The presence of the corresponding receptors on TLS T cells was confirmed. Intratumoral PNAdþ high endothelial venules also were exclusively associated with TLS and colocalized with CD62Lþ lymphocytes. Together, these data bring new insights into the T-cell recruitment to intratumoral TLS and suggest that blood T cell enter into TLS via high endothelial venules, which represent a new gateway for T cells to the tumor. Findings identify the molecules that mediate migration of tumor-specific T cells into TLS where T cell priming occurs, suggesting new strategies to enhance the efficacy of cancer immunotherapies.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of Chemokines and Adhesion Molecules Associated with T cell Presence in Tertiary Lymphoid Structures in Human Lung Cancer

et al. 2011

View full text Add to dashboard Cite

show abstract

“…These chemokines share the CCR4 receptor, which is expressed on central memory Th2 T cells, cutaneous T cells and regulatory T cells [85,86]. CCL17 and CCL22 are able to recruit effector T cells [87,88,89,90] and Tregs in cancer mouse models [90,91,92,93]. Human CCL22 expression is associated with tumor regression [88,89,90,94] and good prognosis in lung cancer [95] whereas in breast cancer, it correlates with Treg infiltration and poor prognosis [91].…”

Section: The Immune Contexture Of Primary and Metastatic Tumorsmentioning

confidence: 99%

The Immune Microenvironment: A Major Player in Human Cancers

Fridman¹,

Remark²,

Goc³

et al. 2014

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Cancer is a major public health issue and figures among the leading causes of death in the world. Cancer development is a long process, involving the mutation, amplification or deletion of genes and chromosomal rearrangements. The transformed cells change morphologically, enlarge, become invasive and finally detach from the primary tumor to metastasize in other organs through the blood and/or lymph. During this process, the tumor cells interact with their microenvironment, which is complex and composed of stromal and immune cells that penetrate the tumor site via blood vessels and lymphoid capillaries. All subsets of immune cells can be found in tumors, but their respective density, functionality and organization vary from one type of tumor to another. Whereas inflammatory cells play a protumoral role, there is a large body of evidence of effector memory T cells controlling tumor invasion and metastasis. Thus, high densities of memory Th1/CD8 cytotoxic T cells in the primary tumors correlate with good prognosis in most tumor types. Tertiary lymphoid structures, which contain mature dendritic cells (DC) in a T cell zone, proliferating B cells and follicular DC, are found in the tumor stroma and they correlate with intratumoral Th1/CD8 T cell and B cell infiltration. Eventually, tumors undergo genetic and epigenetic modifications that allow them to escape being controlled by the immune system. This comprehensive review describes the immune contexture of human primary and metastatic tumors, how it impacts on patient outcomes and how it could be used as a predictive biomarker and guide immunotherapies.

show abstract

“…On the other hand, CCL17 has been linked to accumulation of FOXP3 + regulatory T cells (T regs ) in gastric cancer [129]. In a recent study, selective knock down of CCL22 and CCL17 expression by siRNA decreased the ratios of CD4 + to CD8 + as well as the frequency of T regs recruited by monocyte-derived DCs (MoDCs) [130]. Intratumoral injection of CCL22 and CCL17 knockout DCs significantly reduced infiltration of T regs in tumor.…”

Section: Adenosine and Dendritic Cells Interaction In Tumor Environmentmentioning

confidence: 99%

Adenosine as an endogenous immunoregulator in cancer pathogenesis: where to go?

Kumar

2012

Purinergic Signalling

View full text Add to dashboard Cite

Cancer is a chronic disease and its pathogenesis is well correlated with infection and inflammation. Adenosine is a purine nucleoside, which is produced under metabolic stress like hypoxic conditions. Acute or chronic inflammatory conditions lead to the release of precursor adenine nucleotides (adenosine triphosphate (ATP), adenosien diphosphate (ADP) and adenosine monophosphate (AMP)) from cells, which are extracellularly catabolized into adenosine by extracellular ectonucleotidases, i.e., CD39 or nucleoside triphosphate dephosphorylase (NTPD) and CD73 or 5′-ectonucleotidase. It is now well-known that adenosine is secreted by cancer as well as immune cells during tumor pathogenesis under metabolic stress or hypoxia. Once adenosine is released into the extracellular environment, it exerts various immunomodulatory effects via adenosine receptors (A 1 , A 2A , A 2B , and A 3 ) expressed on various immune cells (i.e., macrophages, myeloid-derived suppressor cells (MDSCs), natural killer (NK) cells, dendritic cells (DCs), T cells, regulatory T cell (T regs ), etc.), which play very important roles in the pathogenesis of cancer. This review is intended to summarize the role of inflammation and adenosine in the immunopathogenesis of tumor along with regulation of tumor-specific immune response and its modulation as an adjunct approach to tumor immunotherapy.

show abstract

Targeted knock down of CCL22 and CCL17 by siRNA during DC differentiation and maturation affects the recruitment of T subsets

Cited by 34 publications

References 49 publications

Characterization of Chemokines and Adhesion Molecules Associated with T cell Presence in Tertiary Lymphoid Structures in Human Lung Cancer

Characterization of Chemokines and Adhesion Molecules Associated with T cell Presence in Tertiary Lymphoid Structures in Human Lung Cancer

The Immune Microenvironment: A Major Player in Human Cancers

Adenosine as an endogenous immunoregulator in cancer pathogenesis: where to go?

Contact Info

Product

Resources

About