Targeted killing of tumor cells based on isoelectric point suitable nanoceria-rod with high oxygen vacancies

Ma, Baofu; Han, Jianyu; Kun, Zhang; Jiang, Qike; Sui, Zhigang; Zhang, Zhixin; Zhao, Baofeng; Liang, Zhen; Zhang, Lihua; Zhang, Yukui

doi:10.1039/d1tb02787e

Cited by 9 publications

(12 citation statements)

References 49 publications

(52 reference statements)

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“…It is necessary to improve the enzyme-like activities of nanoceria further to enable practical treatments. Most previous studies were focused on the morphology or doping to enhance the catalytic activity of nanoceria. , We also found that nanoceria-rods with high enzymatic activity had more powerful tumor-killing capability compared than nanoceria-polyhedra and cubes …”

Section: Introductionsupporting

confidence: 71%

“…The oxygen vacancies of the Pt/CeO 2 -NR and Pt/CeO 2 -R nanozymes were characterized by Raman spectroscopy. The intensity ratio for the peaks at 595 cm –1 (IVo) and approximately 465 cm –1 (IF2g) were used to quantify the surface concentration of oxygen vacancies. , As Figure a shows, the single-atom Pt/CeO 2 -NR and CeO 2 -NR carriers had lower oxygen vacancy concentrations (IVo/IF2g, Pt/CeO 2 -NR 0.054 compared with CeO 2 -NR 0.012). After H 2 reduction at 600 °C, the oxygen vacancy concentrations of Pt/CeO 2 -R and CeO 2 -R were increased (Ivo/IF2g, Pt/CeO 2 -R 0.114 compared with CeO 2 -R 0.023) (Figure b).…”

Section: Resultsmentioning

confidence: 99%

“…Prior studies indicated that nanozymes enter tumor cells, and then boosts ROS generation and induces tumor cell apoptosis. , An ROS detection kit was used to assess the cellular levels of ROS after the Pt/CeO 2 -R treatment. The results showed that Pt/CeO 2 -R produced large amounts of ROS in tumor cells (Figure S8), consistent with the high POD and OXD-like activities seen in acidic environments.…”

Section: Resultsmentioning

confidence: 99%

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Platinum Engineering on Nanoceria Surface toward High Catalytic Activity for Inducing Tumor Cell Apoptosis and Inhibiting Migration

Han

Zhao

et al. 2023

ACS Sustainable Chem. Eng.

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Nanozymes have shown great clinical application potential in the treatment of tumors with reactive oxygen species (ROS). However, conventional nanozyme technologies face challenges such as low catalytic activity and unknown therapeutic mechanisms for tumor therapy. Herein, we designed platinum (Pt)/nanoceria (CeO 2 ) hybrid nanozymes using Pt single-atom and cluster engineering on the CeO 2 surface, named Pt/CeO 2 -NR and Pt/CeO 2 -R respectively. The results showed that Pt/CeO 2 -R had higher enzyme-like activity than Pt/CeO 2 -NR because the Pt clusters (Pt 0 and Pt 2+ ) stabilized the oxygen vacancies better than the single Pt atoms (Pt 2+ ). Further studies showed that Pt/CeO 2 -R exhibited selective toxicity to tumor cells, induced tumor cell apoptosis, and inhibited cell migration. In addition, we studied the antitumor mechanism of Pt/CeO 2 -R with stable isotopic labeling by amino acids in cell culture (SILAC)-based quantitative proteomics. Mechanistically, large amounts of the ROS produced by Pt/ CeO 2 -R affected both the mitochondria and the endoplasmic reticulum and ultimately induced tumor cell apoptosis. Additionally, Pt/CeO 2 -R inhibited tumor cell migration by downregulating the proteins on the cell membrane surface. This study provided an approach for optimizing the enzyme-like activities of CeO 2 through the efficient use of platinum engineering, and explored the mechanisms by which Pt/CeO 2 -R killed tumor cells and inhibited cell migration during the treatment of tumors.

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Section: Introductionsupporting

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Platinum Engineering on Nanoceria Surface toward High Catalytic Activity for Inducing Tumor Cell Apoptosis and Inhibiting Migration

Han

Zhao

et al. 2023

ACS Sustainable Chem. Eng.

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“…[ 174,176 ] The principle of crystal defects indicates that the local bonding and electronic properties of matrix metals may be changed by site‐specific defect engineering by the doping of different metal ions, thereby enhancing the recognition and response to different pH conditions (Figure 10b). [ 174,190,191 ] CeO 2 NPs had low POD‐like activity until pH gradually dropped below 5, [ 192 ] while Fe 2+ ‐doped CeO 2 nanozymes changed their valence state more sensitively. In particular, Fe 2+ ‐doped CeO 2 converted to Ce 3+ when pH = 6.5 to exert POD‐like activity and produce ROS, while it could also change to Ce 4+ when pH = 7.4 to affect CAT‐like activity, thus removing ROS and reducing oxidative stress in inflamed tissues.…”

Section: Optimization Strategy For the Efficacy Of Mnmsmentioning

confidence: 99%

Metal‐Based Nanozymes with Multienzyme‐Like Activities as Therapeutic Candidates: Applications, Mechanisms, and Optimization Strategy

Dai

Xiong

et al. 2022

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manganese(III) oxide (Mn 2 O 3 ), and Prussian blue nanozyme (PBzyme), etc., have multienzyme-like activities, which enable either the efficient production of reactive oxygen species (ROS) to kill tumor cells and bacteria, or the clearance of ROS to reduce oxidative stress and inflammation. [9][10][11][12][13][14] These metal-based nanozymes display multiple antitumor, anti-infective, and anti-inflammatory activities, and offer promise as potential adjuvants, cotreatments, or alternatives to cytotoxic chemotherapeutics, antibiotics, and nonsteroidal anti-inflammatory drugs. [15][16][17] Most nanozymes in preclinical development have single-enzyme-like activity, and are thus limited by insufficient catalytic efficiency, restricted availability of substrate types and concentrations, and activity under single identifiable catalytic conditions. In comparison, those with multienzyme-like activities carry advantages of high catalytic efficiency via amplification or cascade reactions, adaptive responses to dissimilar catalytic conditions, multifunctionality in diverse pathological processes, and capacities to overcome the impediment of insufficient substrates via "self-provision." [18] At present, most reported nanozymes with multienzyme-like activities are metal-based, owing to their unsaturated sites from the inherent structure of metal atoms and the valence changes of the metal centers in case of transition metals. [4] In the last decade, researchers have studied catalytic effects and regulation rules and design schemes of metal-based nanozymes with multienzyme-like activities (MNMs), with goals to remarkably improve their therapeutic efficiency and broaden their range of applications. [15,[19][20][21][22][23][24][25] Previous review articles have discussed the spatial structures and catalytic mechanisms of nanozymes from the perspective of their physical properties, or have summarized the effects of a few types of nanozymes with potential medical applications. [4,6,[26][27][28][29][30][31][32] However, clinical relevance stands as the core motivator of nanozyme research. Therefore, this review concentrates on MNMs. This work focuses on the biological effects of nanozymes based on their intracellular interactions, intending to analyze mechanisms of action and potential therapeutic applications. Herein, we summarize MNMs, their impacts on pathogenesis, underlying mechanisms, potential medical Most nanozymes in development for medical applications only exhibit singleenzyme-like activity, and are thus limited by insufficient catalytic activity and dysfunctionality in complex pathological microenvironments. To overcome the impediments of limited substrate availabilities and concentrations, some metal-based nanozymes may mimic two or more activities of natural enzymes to catalyze cascade reactions or to catalyze multiple substrates simultaneously, thereby amplifying catalysis. Metal-based nanozymes with multienzyme-like activities (MNMs) may adapt to dissimilar catalytic conditions to exert different enzyme-like effects. ...

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“…Nanozymes with multiple enzymatic activities can effectively produce reactive oxygen species in the tumor microenvironment through cascade catalytic reactions, thereby promoting the apoptosis of cancer cells (Zhao et al, 2021b;Yao et al, 2022b;Wang et al, 2022f). Ma et al (2022) designed nanozymes with three nanoceria structures including nanoceria-cube, nanoceria-poly and nanoceria-rod. Among them, nanoceria-rod with highest concentration of surface oxygen vacancies has the highest POD-like and OXD-like enzyme activity.…”

Section: Apoptosis Inductionmentioning

confidence: 99%

Progress and prospects of nanozymes for enhanced antitumor therapy

Zhao

Yuan

et al. 2022

Front. Chem.

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Nanozymes are nanomaterials with mimicked enzymatic activity, whose catalytic activity can be designed by changing their physical parameters and chemical composition. With the development of biomedical and material science, artificially created nanozymes have high biocompatibility and can catalyze specific biochemical reactions under biological conditions, thus playing a vital role in regulating physiological activities. Under pathological conditions, natural enzymes are limited in their catalytic capacity by the varying reaction conditions. In contrast, compared to natural enzymes, nanozymes have advantages such as high stability, simplicity of modification, targeting ability, and versatility. As a result, the novel role of nanozymes in medicine, especially in tumor therapy, is gaining increasing attention. In this review, function and application of various nanozymes in the treatment of cancer are summarized. Future exploration paths of nanozymes in cancer therapies based on new insights arising from recent research are outlined.

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Targeted killing of tumor cells based on isoelectric point suitable nanoceria-rod with high oxygen vacancies

Abstract: Nanozymes have great potential application in tumor treatment because of their good stability, high biocompatibility, easy preparation and versatility. However, it remains a challenge to design of highly active nanozyme...

Cited by 9 publications

References 49 publications

Platinum Engineering on Nanoceria Surface toward High Catalytic Activity for Inducing Tumor Cell Apoptosis and Inhibiting Migration

Platinum Engineering on Nanoceria Surface toward High Catalytic Activity for Inducing Tumor Cell Apoptosis and Inhibiting Migration

Metal‐Based Nanozymes with Multienzyme‐Like Activities as Therapeutic Candidates: Applications, Mechanisms, and Optimization Strategy

Progress and prospects of nanozymes for enhanced antitumor therapy

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