Targeted inhibition of allergen‐induced histamine production by neutrophils

Chacón, Pedro; Vega-Rioja, Antonio; Doukkali, Bouchra; Rodríguez, Alberto del Valle; Bellido, V.; Puente, Yolanda Gutiérrez; Alcañiz, Lorena; Rodríguez, David; Palácios, Ricardo; Cornejo-García, José Antonio; Monteseirı́n, Javier; Rivas-Pérez, David

doi:10.1096/fj.202001912r

Cited by 3 publications

(8 citation statements)

References 63 publications

(130 reference statements)

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“…In this work we show the cell-signaling pathways involved in ECP production, in order to provide possible therapeutic targets. We found that PI3K and ERK1/2 MAPKS are activated in response to Ags (see Figure 5 ), matching previous results from our laboratory ( 24 , 25 ). They are both involved in the IgE-dependent L-selectin downmodulation ( 50 ), NF-κB activation, COX-2 expression ( 25 ), MMP-9 ( 51 ) and histamine release ( 24 ).…”

Section: Discussionsupporting

confidence: 91%

“…We found that PI3K and ERK1/2 MAPKS are activated in response to Ags (see Figure 5 ), matching previous results from our laboratory ( 24 , 25 ). They are both involved in the IgE-dependent L-selectin downmodulation ( 50 ), NF-κB activation, COX-2 expression ( 25 ), MMP-9 ( 51 ) and histamine release ( 24 ). In agreement with these results, PI3K and ERK1/2 MAPK are also required for Toll-like receptor-dependent ECP production by human eosinophils ( 52 ).…”

Section: Discussionsupporting

confidence: 91%

“…PI3K and MAPKs regulate the functional assembly of NADPH oxidase (NOX-2) ( 22 ), an enzyme producing Radical Oxygen Species (ROS), second messengers involved in the priming and degranulation of neutrophils ( 23 ). We have previously shown that PI3K, MAPKs and NOX-2 pathways are activated in neutrophils from allergic patients by Ags/α-IgE ( 24 , 25 ).…”

Section: Resultsmentioning

confidence: 99%

“…In human neutrophils, the expression of glucocorticoid receptors ( 58 ) controls neutrophil activation, migration, respiratory burst, and apoptosis ( 59 ). In addition, they regulate IgE-dependent histamine production by neutrophils from allergic patients ( 24 ). Here we show that budesonide inhibits Ags/α-IgE-dependent ECP production.…”

Section: Discussionmentioning

confidence: 99%

“…β 2 -adrenergic (activated by LABAs) and M3-muscarinic receptors (inactivated by LAMAs) are also expressed by neutrophils ( 61 , 62 ). Signaling through these receptors reduces inflammation through the inhibition of cytokine production, chemotaxis ( 63 , 64 ), and histamine release ( 24 ). As with eosinophils, we found a partial inhibition with the LABA formoterol ( 65 ).…”

Section: Discussionmentioning

confidence: 99%

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Regulation and directed inhibition of ECP production by human neutrophils

et al. 2022

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BackgroundNeutrophils are involved in the pathophysiology of allergic asthma, where the Eosinophil Cationic Protein (ECP) is a critical inflammatory mediator. Although ECP production is attributed to eosinophils, we reported that ECP is also present in neutrophils from allergic patients where, in contrast to eosinophils, it is produced in an IgE-dependent manner. Given the key role of ECP in asthma, we investigated the molecular mechanisms involved in ECP production as well as the effects induced by agonists and widely used clinical approaches. We also analyzed the correlation between ECP production and lung function.MethodsNeutrophils from allergic asthmatic patients were challenged with allergens, alone or in combination with cytokines, in the presence of cell-signaling inhibitors and clinical drugs. We analyzed ECP levels by ELISA and confocal microscopy. Lung function was assessed by spirometry.ResultsIgE-mediated ECP release is dependent on phosphoinositide 3-kinase, the extracellular signal-regulated kinase (ERK1/2) and the production of reactive oxygen species by NADPH-oxidase. Calcineurin phosphatase and the transcription factor NFAT are also involved. ECP release is enhanced by the cytokines interleukin (IL)-5 and granulocyte macrophage-colony stimulating factor, and inhibited by interferon-γ, IL-10, clinical drugs (formoterol, tiotropium and budesonide) and allergen-specific IT. We also found an inverse correlation between asthma severity and ECP levels.ConclusionsOur results suggest the molecular pathways involved in ECP production and potential therapeutic targets. We also provide a new method to evaluate disease severity in asthmatic patients based on the quantification of in vitro ECP production by peripheral neutrophils.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Regulation and directed inhibition of ECP production by human neutrophils

et al. 2022

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Broiler Spaghetti Meat Abnormalities: Muscle Characteristics and Metabolomic Profiles

Wu,

Liu,

et al. 2024

Animals

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Spaghetti meat (SM) is a newly identified muscle abnormality that significantly affects modern broiler chickens, consequently exerting a substantial economic impact on the poultry industry worldwide. However, investigations into the meat quality and the underlying causative factors of SM in broilers remain limited. Therefore, this study was undertaken to systematically evaluate meat quality and muscle fiber characteristics of SM-affected meat. To elucidate the disparities between SM-affected and normal (NO) muscles in broiler chickens reared under identical conditions, we selected 18 SM-affected breast tissues and 18 NO breast tissues from 200 broiler chickens raised according to commercial standards under the same conditions for our study. The results showed that compared with the NO group, the muscle surface of the SM group lost integrity, similar to strip and paste. The brightness and yellowness values were significantly higher than those of the NO group. On the contrary, the shear force and protein were significantly lower in the SM group. Microscopic examination revealed that the muscle fibers in the SM group were lysed, necrotic, and separated from each other, with a large number of neutrophils diffusely distributed on the sarcolemma and endometrium. Thirty-five significantly different metabolites were observed in the breast muscles between both groups. Among them, the top differential metabolites—14,15-DiHETrE, isotretinoin, L-malic acid, and acetylcysteine—were mainly enriched in lipid metabolism and inflammatory pathways, including linoleic acid, arachidonic acid, phenylalanine, and histidine metabolism. Overall, these findings not only offer new insights into the meat quality and fiber traits of SM but also contribute to the understanding of potential mechanisms and nutritional regulators for SM myopathy.

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Eosinophilic Cationic Protein and Immunoglobulin E: Unraveling Biomarkers in Chronic Pediatric Cough

Lazova,

Naydenova,

Velikova

2024

Sinusitis

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Although the cough reflex is one of the essential protective mechanisms in the respiratory tract, it is considered a considerable health problem in adults and children when it becomes chronic and hypersensitive. However, the need for biomarkers for chronic cough in children and adults is critical. The problem with cough is also a severe symptom in hypersensitivity children. Respiratory infections are a considerable challenge for pediatricians, especially in allergic children. The term cough hypersensitivity syndrome, although introduced in adults, was questioned for children. Eosinophil cationic protein (ECP) is a promising marker for chronic cough but still needs to be validated and proved in clinical settings. In this review article, we aimed to discuss the possible role of ECP in connection to IgE for chronic cough in children.

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Targeted inhibition of allergen‐induced histamine production by neutrophils

Cited by 3 publications

References 63 publications

Regulation and directed inhibition of ECP production by human neutrophils

Regulation and directed inhibition of ECP production by human neutrophils

Broiler Spaghetti Meat Abnormalities: Muscle Characteristics and Metabolomic Profiles

Eosinophilic Cationic Protein and Immunoglobulin E: Unraveling Biomarkers in Chronic Pediatric Cough

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