Targeted in vivo photodynamic therapy with epidermal growth factor receptor-specific peptide linked nanoparticles

Amreddy, Narsireddy; Vijayashree, Kurra; Irudayaraj, Joseph; Manorama, Sunkara V.; Rao, Nalam Madhusudhana

doi:10.1016/j.ijpharm.2014.05.063

Cited by 29 publications

(20 citation statements)

References 56 publications

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“…These nanoparticles can be prepared in smaller sizes by simple chemical methods [86], [87]. The surfaces of magnetic nanoparticles can be modified with different types of polymers that could help covalently or non-covalently attach siRNA or DNA and protect them from degradation.…”

Section: Inorganic Nanoparticles For Gene Therapymentioning

confidence: 99%

Nanoparticles for siRNA-Based Gene Silencing in Tumor Therapy

Babu

Muralidharan

Amreddy

et al. 2016

IEEE Trans.on Nanobioscience

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Gene silencing through RNA interference (RNAi) has emerged as a potential strategy in manipulating cancer causing genes by complementary base-pairing mechanism. Small interfering RNA (siRNA) is an important RNAi tool that has found significant application in cancer therapy. However due to lack of stability, poor cellular uptake and high probability of loss-of-function due to degradation, siRNA therapeutic strategies seek safe and efficient delivery vehicles for in vivo applications. The current review discusses various nanoparticle systems currently used for siRNA delivery for cancer therapy, with emphasis on liposome based gene delivery systems. The discussion also includes various methods availed to improve nanoparticle based-siRNA delivery with target specificity and superior efficiency. Further this review describes challenges and perspectives on the development of safe and efficient nanoparticle based-siRNA-delivery systems for cancer therapy.

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Section: Inorganic Nanoparticles For Gene Therapymentioning

confidence: 99%

Nanoparticles for siRNA-Based Gene Silencing in Tumor Therapy

Babu

Muralidharan

Amreddy

et al. 2016

IEEE Trans.on Nanobioscience

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“…To prepare nanoparticle-drug conjugates for loading in exosomes, gold nanoparticles (GNPs) may be suitable since they are one of the most studied nanoparticle systems for therapeutic delivery and other biological applications22232425. The smaller size, easy to synthesize, biologically inert and the presence of abundant functional groups for drug conjugation are some the advantages of GNP262728.…”

mentioning

confidence: 99%

Nanosomes carrying doxorubicin exhibit potent anticancer activity against human lung cancer cells

Srivastava

Amreddy

Babu

et al. 2016

Sci Rep

145

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Successful chemotherapeutic intervention for management of lung cancer requires an efficient drug delivery system. Gold nanoparticles (GNPs) can incorporate various therapeutics; however, GNPs have limitations as drug carriers. Nano-sized cellular vesicles like exosomes (Exo) can ferry GNP-therapeutic complexes without causing any particle aggregation or immune response. In the present study, we describe the development and testing of a novel Exo-GNP-based therapeutic delivery system -‘nanosomes’- for lung cancer therapy. This system consists of GNPs conjugated to anticancer drug doxorubicin (Dox) by a pH-cleavable bond that is physically loaded onto the exosomes (Exo-GNP-Dox). The therapeutic efficacy of Dox in nanosomes was assessed in H1299 and A549 non-small cell lung cancer cells, normal MRC9 lung fibroblasts, and Dox-sensitive human coronary artery smooth muscle cells (HCASM). The enhanced rate of drug release under acidic conditions, successful uptake of the nanosomes by the recipient cells and the cell viability assays demonstrated that nanosomes exhibit preferential cytotoxicity towards cancer cells and have minimal activity on non-cancerous cells. Finally, the underlying mechanism of cytotoxicity involved ROS-mediated DNA damage. Results from this study mark the establishment of an amenable drug delivery vehicle and highlight the advantages of a natural drug carrier that demonstrates reduced cellular toxicity and efficient delivery of therapeutics to cancer cells.

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“…Thus, EGFR expression in lung cancer cells has been explored for targeted gene delivery using nanoparticles. EGFR antibody or affibody can be used for EGFR-targeted delivery of therapeutics to lung cancer cells [116]. The targeting EGFR molecules can be conjugated on the surface of the nanoparticles through covalent or non-covalent interactions.…”

Section: Polymeric Nanoparticles For Receptor-mediated Gene Delivery mentioning

confidence: 99%

Polymeric Nanoparticle-Mediated Gene Delivery for Lung Cancer Treatment

et al. 2017

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In recent years, researchers have focused on targeted gene therapy for lung cancer, using nanoparticle carriers to overcome the limitations of conventional treatment methods. The main goal of targeted gene therapy is to develop more efficient therapeutic strategies by improving the bioavailability, stability, and target specificity of gene therapeutics and to reduce off-target effects. Polymer-based nanoparticles, an alternative to lipid and inorganic nanoparticles, efficiently carry nucleic acid therapeutics and are stable in vivo. Receptor-targeted delivery is a promising approach that can limit non-specific gene delivery and can be achieved by modifying the polymer nanoparticle surface with specific receptor ligands or antibodies. This review highlights the recent developments in gene delivery using synthetic and natural polymer-based nucleic acid carriers for lung cancer treatment. Various nanoparticle systems based on polymers and polymer combinations are discussed. Further, examples of targeting ligands or moieties used in targeted, polymer-based gene delivery to lung cancer are reviewed.

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Targeted in vivo photodynamic therapy with epidermal growth factor receptor-specific peptide linked nanoparticles

Cited by 29 publications

References 56 publications

Nanoparticles for siRNA-Based Gene Silencing in Tumor Therapy

Nanoparticles for siRNA-Based Gene Silencing in Tumor Therapy

Nanosomes carrying doxorubicin exhibit potent anticancer activity against human lung cancer cells

Polymeric Nanoparticle-Mediated Gene Delivery for Lung Cancer Treatment

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