Targeted in situ metatranscriptomics for selected taxa from mesophilic and thermophilic biogas plants

Abstract

SummaryBiogas production is performed anaerobically by complex microbial communities with key species driving the process. Hence, analyses of their in situ activities are crucial to understand the process. In a previous study, metagenome sequencing and subsequent genome binning for different production‐scale biogas plants (BGPs) resulted in four genome bins of special interest, assigned to the phyla Thermotogae, Fusobacteria, Spirochaetes and Cloacimonetes, respectively, that were genetically analysed. In this study, metatranscriptome sequencing of the same BGP samples was conducted, enabling in situ transcriptional activity determination of these genome bins. For this, mapping of metatranscriptome reads on genome bin sequences was performed providing transcripts per million (TPM) values for each gene. This approach revealed an active sugar‐based metabolism of the Thermotogae and Spirochaetes bins and an active amino acid‐based metabolism of the Fusobacteria and Cloacimonetes bins. The data also hint at syntrophic associations of the four corresponding species with methanogenic Archaea.

Microbial Community Changes in a Chlorinated Solvents Polluted Aquifer Over the Field Scale Treatment With Poly-3-Hydroxybutyrate as Amendment

“…It is worth noting the occurrence of low-abundance OTUs affiliated to poorly characterized bacterial lineages such as Aminicenantes, Microgenomates , and Cloacimonetes phyla previously found in a wide variety of anaerobic environments including oil reservoirs and hydrocarbon-impacted sites ( Farag et al, 2014 ; Hu et al, 2016 ; Stolze et al, 2017 ).…”

Operational Parameters of Biogas Plants: A Review and Evaluation Study

“…In Europe, the biogas is mainly produced by the anaerobic digestion of agricultural residuals, manure, and energy crops. Additionally, the sludge of wastewater treatment plants, organic fraction of the municipal solid waste, or solid waste buried in landfills are possible feedstock sources [10,13,15]. The anaerobic digestion is an efficient method to treat the organic fraction of the municipal solid waste for energy production while mitigating the greenhouse emissions [16][17][18].…”

“…Anaerobic digestion is a biological process, in which the microorganisms degrade the complex organic matter to simpler components under anaerobic conditions to produce biogas and fertilizer [6,8]. This process has many environmental benefits, such as green energy production, organic waste treatment, environmental protection, and greenhouse gas emissions reduction [2,[9][10][11][12][13]. The biodegradation of the complex organic matter undergoes four main steps.…”

Genome Analyses and Genome-Centered Metatranscriptomics of Methanothermobacter wolfeii Strain SIV6, Isolated from a Thermophilic Production-Scale Biogas Fermenter

“…To analyze the transcriptional activity of M. wolfeii SIV6 within the biogas fermenter, a mapping of metatranscriptome reads obtained from the corresponding biogas fermenter was accomplished. Total microbial RNA extraction and metatranscriptome sequencing was done as described in detail by Stolze et al (2018) [33]. The RNA was isolated from the same fermenter sample as the M. wolfeii SIV6 strain.…”