Targeted hyaluronic acid-based lipid nanoparticle for apigenin delivery to induce Nrf2-dependent apoptosis in lung cancer cells

Mahmoudi, Shiva; Ghorbani, Marjan; Sabzichi, Mehdi; Ramezani, Fatemeh; Hamishehkar, Hamed; Samadi, Nasser

doi:10.1016/j.jddst.2018.11.013

Cited by 69 publications

(28 citation statements)

References 48 publications

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“…Annexin V/propidium iodide (PI) staining was carried out according to the product instruction. Briefly, 10 µL Annexin V‐fluorescein isothiocyanate (FITC) and 10 µL PI were added to the samples for 15 minutes at room temperature in the dark place . Flow cytometric analysis was accomplished using a BD FACSCalibur (BD Biosciences, Franklin Lakes, NJ).…”

Section: Methodsmentioning

confidence: 99%

Adjuvant therapy with γ‐tocopherol‐induce apoptosis in HT‐29 colon cancer via cyclin‐dependent cell cycle arrest mechanism

Bazzaz

Bijanpour

Pirouzpanah

et al. 2019

J Biochem & Molecular Tox

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Resistance to chemotherapy with 5-fluorouracil (5-FU) in patients with colorectal cancer (CRC) is the major obstacle to reach the maximum efficiency of CRC treatment. Combination therapy has emerged as a novel anticancer strategy. The present study evaluates the cotreatment of γ-tocopherol and 5-FU in enhancing the efficacy of chemotherapy against HT-29 colon cancer cells. Cytotoxic effect of this combination was examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide assay and a synergistic effect was evaluated by a combination index technique. Nuclear morphology was studied via 4′,6diamidino-2-phenylindole staining and flow cytometric assays were conducted to identify molecular mechanisms of apoptosis and cell cycle progression. We investigated the expression of Cyclin D1, Cyclin E, Bax, and Bcl-2 by a quantitative real-time polymerase chain reaction. The IC 50 values for 5-FU and γ-tocopherol were 21.8 ± 2.5 and 14.4 ± 2.6 μM, respectively, and also this combination therapeutic increased the percentage of apoptotic cells from 35% ± 2% to 40% ± 4% (P < .05). Furthermore, incubation HT-29 colon cells with combined concentrations of two drugs caused significant accumulation of cells in the subGsubG1 phase. Our results presented the combination therapy with 5-FU and γ-tocopherol as a novel therapeutic approach, which can enhance the efficacy of chemotherapy. K E Y W O R D S 5-fluorouracil, apoptosis, cancer, combination therapy, γ-tocopherol

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Section: Methodsmentioning

confidence: 99%

Adjuvant therapy with γ‐tocopherol‐induce apoptosis in HT‐29 colon cancer via cyclin‐dependent cell cycle arrest mechanism

Bazzaz

Bijanpour

Pirouzpanah

et al. 2019

J Biochem & Molecular Tox

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“…The LbL assembly of PLL-HA is pH sensitive: it is disrupted in the acidic tumor microenvironment, leading to the release of the loaded therapeutic agent [36]. Drugs with low water solubility are usually difficult to deliver in the cellular milieu; however, they can be solubilized using electrostatically decorated nanostructured lipid carriers, with HA as a targeting ligand for lung cancer cells [37]. HA can also be presented using gold nanoparticles conjugated with end-thiolated HA (HS-HA) to promote its binding to the gold surface, generating a brush-like surface structure [38].…”

Section: Box 2 Ha In Human Tissues and Associated Diseasesmentioning

confidence: 99%

Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials

Amorim

Reis

et al. 2021

Trends in Biotechnology

113

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Hyaluronan (HA) is a critical element of the extracellular matrix (ECM). The regulated synthesis and degradation of HA modulates the ECM chemical and physical properties that, in turn, influence cellular behavior. HA triggers signaling pathways associated with the adhesion, proliferation, migration, and differentiation of cells, mediated by its interaction with specific cellular receptors or by tuning the mechanical properties of the ECM. This review summarizes the recent advances on strategies used to mimic the HA present in the ECM to study healthy or pathological cellular behavior. This includes the development of HA-based 2D and 3D in vitro tissue models for the seeding and encapsulation of cells, respectively, and HA particles as carriers for the targeted delivery of therapeutic agents. Highlights The extracellular matrix is composed of hyaluronan of different molecular weights that play different roles in cellular proliferation, migration, and differentiation.

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“…Overcoming poor bioavailability and low solubility of apigenin requires using carriers for targeted delivery of apigenin that remarkably enhances the anti-tumor activity of apigenin (Mahmoudi et al, 2019). Fortunately, different nanocarriers were designed for the delivery of apigenin and for reducing the survival and viability of cancer cells.…”

Section: Apigenin-loaded Nanovehiclesmentioning

confidence: 99%

“…On the other hand, as mentioned before, resistance of cancer cells to chemotherapy is an increasing challenge in the field of cancer therapy. Hence, using carriers for protecting and delivering of biotherapeutic agents not only ameliorates its anti-tumor activity but also sensitizes cancer cells to the inhibitory effects of chemotherapeutic agents (Mahmoudi et al, 2019;Jamaledin et al, 2020).…”

Section: Apigenin-loaded Nanovehiclesmentioning

confidence: 99%

“…On the other hand, HA functionalization of nanovehicles can indeed change physicochemical properties, alter stability, toxicity, and influence nanoparticle biodistribution and efficiency in vitro and in vivo (Wang et al, 2016;Xu et al, 2017;Alves et al, 2019). In light of this, HA decorated-lipid nanoparticles were employed for the delivery of anti-tumor therapeutics (Figure 7) (Mahmoudi et al, 2019). The finding showed that co-delivery of apigenin and docetaxel impose synergistic anti-cancer effects toward A549 cells.…”

Section: Apigenin-loaded Nanovehiclesmentioning

confidence: 99%

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Apigenin as Tumor Suppressor in Cancers: Biotherapeutic Activity, Nanodelivery, and Mechanisms With Emphasis on Pancreatic Cancer

et al. 2020

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Pancreatic cancer is the most lethal malignancy of the gastrointestinal tract. Due to its propensity for early local and distant spread, affected patients possess extremely poor prognosis. Currently applied treatments are not effective enough to eradicate all cancer cells, and minimize their migration. Besides, these treatments are associated with adverse effects on normal cells and organs. These therapies are not able to increase the overall survival rate of patients; hence, finding novel adjuvants or alternatives is so essential. Up to now, medicinal herbs were utilized for therapeutic goals. Herbal-based medicine, as traditional biotherapeutics, were employed for cancer treatment. Of them, apigenin, as a bioactive flavonoid that possesses numerous biological properties (e.g., anti-inflammatory and anti-oxidant effects), has shown substantial anticancer activity. It seems that apigenin is capable of suppressing the proliferation of cancer cells via the induction of cell cycle arrest and apoptosis. Besides, apigenin inhibits metastasis via down-regulation of matrix metalloproteinases and the Akt signaling pathway. In pancreatic cancer cells, apigenin sensitizes cells in chemotherapy, and affects molecular pathways such as the hypoxia inducible factor (HIF), vascular endothelial growth factor (VEGF), and glucose transporter-1 (GLUT-1). Herein, the biotherapeutic activity of apigenin and its mechanisms toward cancer cells are presented in the current review to shed some light on anti-tumor activity of apigenin in different cancers, with an emphasis on pancreatic cancer.

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Targeted hyaluronic acid-based lipid nanoparticle for apigenin delivery to induce Nrf2-dependent apoptosis in lung cancer cells

Cited by 69 publications

References 48 publications

Adjuvant therapy with γ‐tocopherol‐induce apoptosis in HT‐29 colon cancer via cyclin‐dependent cell cycle arrest mechanism

Adjuvant therapy with γ‐tocopherol‐induce apoptosis in HT‐29 colon cancer via cyclin‐dependent cell cycle arrest mechanism

Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials

Apigenin as Tumor Suppressor in Cancers: Biotherapeutic Activity, Nanodelivery, and Mechanisms With Emphasis on Pancreatic Cancer

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