Targeted HPTLC Profile, Quantification of Flavonoids and Phenolic Acids, and Antimicrobial Activity of Dodonaea angustifolia (L.f.) Leaves and Flowers

Abstract

In East Africa, Dodonaea angustifolia (L.f.) is a well-known medicinal herb. Its leaf is primarily studied in light of its ethnobotanical use. In terms of phytochemistry and biological activity, its flower is not studied. In a prior study, our team looked into phytochemical screening, antioxidant activity, and total phenolic levels. This study aims to compare the profiles and biological activities of the leaf and flower samples of D. angustifolia and to present therapeutic alternatives. The leaf and flower sample powders were extracted with methanol using ultrasound-assisted extraction (UAE). HPTLC profile was obtained using CAMAG—HPTLC equipped with VisionCATS software. Antimicrobial agar well diffusion assay and minimum inhibition concentration (MIC) were determined. The leaf and flower extracts of D. angustifolia showed antibacterial activity with a MIC value of 20 µg/mL against Enterococcus faecalis and Listeria monocytogenes. Similarly, 40 µg/mL was found to be effective against Aspergillus flavus. D. angustifolia flower is a rich source of flavonoids and phenolic acids. Because of its antibacterial properties and profile, which are almost the same, the flower is emerging as a viable option for medicinal alternatives.

Optimization, Identification, and Quantification of Selected Phenolics in Three Underutilized Exotic Edible Fruits Using HPTLC

“…Therefore, the current investigation addresses this research gap through the HPTLC profiling and quantification of the three fruits, the seed, and the peel. The phenolic contents of the studied wild edible fruits were comparable to the reported medicinal plants, as indicated by the previously reported values of 0.0801 to 0.2182 µg/100 mL [35]. result, the SST correlations (R 2 ) of most of the standards with the target compounds in the samples ranged from 0.57 to 0.999%.…”

“…The fingerprints of the HPTLC chromatograms (overlay and stacked) obtained from the previously optimized UAE of the dried fruit powders showed that most of the targeted flavonoids and phenolic acids were identified in most of the fruit powders (Figure 2a-d). The obtained fingerprint of the HPTLC chromatogram (Figure 2a-d) of fruit extracts represents unique biologically active components [35]. The HPTLC-enhanced scans (photograph) of the developed plates (chromatograms) revealed the presence of gallic acid, chlorogenic acid, syringic acid, quercetin, myricetin, kaempferol, and rutin in many of the different dried fruits (Figure 2a-d).…”

“…Vertical development, after preconditioning for 5 min, conditioning for 5 min, saturation for 10 min, and room temperature humidity control of 43 ± 4% with the optimized MP, was successfully applied and well-resolved components were obtained for both standards and sample extracts (Figures 1a-c and 2a-d). Several solvent systems have been reported for the simultaneous identification and quantification of bioactive compounds in fruits, vegetables, and medicinal plants [33,35,[43][44][45].…”

“…Several solvent systems have been reported for the simultaneous identification and quantification of bioactive compounds in fruits, vegetables, and medicinal plants [33,34]. Previously, HPTLC methods were successfully applied for the simultaneous separation and profiling of three phenolic acids and four flavonoids in the leaves and flowers of the medicinal plant Dodonaea angustifolia (L.f.) [35].…”

Controlling Multi-Drug-Resistant Traits of Salmonella Obtained from Retail Poultry Shops Using Metal–Organic Framework (MOF) as a Novel Technique

“…Some reports have predicted that deaths due to antimicrobial-resistant bacteria will reach up to 10 million in the year 2050 [8]. Several synthesized substances have been used to fight microbes and bacteria (e.g., Shigella) [9,10].…”