Targeted genome engineering techniques in Drosophila

Beumer, Kelly J.; Carroll, Dana

doi:10.1016/j.ymeth.2013.12.002

Cited by 65 publications

(51 citation statements)

References 99 publications

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“…Genome-editing technologies have enabled significant progress toward the goal of a complete collection of mutant strains for every gene. Several different technologies have been successfully used in D. melanogaster, including zinc finger directed nucleases, and transcription activator like effector nucleases (TALENs) (Beumer and Carroll 2014). The most promising editing technology is the modified bacterial clustered regularly interspaced short palindromic repeat (CRISPR/Cas9) system.…”

Section: A Drosophila Genetic Toolkitmentioning

confidence: 99%

Genetics on the Fly: A Primer on theDrosophilaModel System

et al. 2015

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Fruit flies of the genus Drosophila have been an attractive and effective genetic model organism since Thomas Hunt Morgan and colleagues made seminal discoveries with them a century ago. Work with Drosophila has enabled dramatic advances in cell and developmental biology, neurobiology and behavior, molecular biology, evolutionary and population genetics, and other fields. With more tissue types and observable behaviors than in other short-generation model organisms, and with vast genome data available for many species within the genus, the fly's tractable complexity will continue to enable exciting opportunities to explore mechanisms of complex developmental programs, behaviors, and broader evolutionary questions. This primer describes the organism's natural history, the features of sequenced genomes within the genus, the wide range of available genetic tools and online resources, the types of biological questions Drosophila can help address, and historical milestones.

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Section: A Drosophila Genetic Toolkitmentioning

confidence: 99%

Genetics on the Fly: A Primer on theDrosophilaModel System

et al. 2015

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“…Because all the targetable nucleases make DSBs and the basic features of DNA repair pathways are shared among eukaryotic organisms, we expect that the lessons learned will be broadly applicable. We have recently published a description of procedures for using targetable nucleases in Drosophila [21], and the reader is referred to that article for experimental details.…”

Section: Experimental Observations In Drosophilamentioning

confidence: 99%

Genome engineering with TALENs and ZFNs: Repair pathways and donor design

Carroll

Beumer

2014

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Genome engineering with targetable nucleases depends on cellular pathways of DNA repair after target cleavage. Knowledge of how those pathways work, their requirements and their active factors, can guide experimental design and improve outcomes. While many aspects of both homologous recombination (HR) and nonhomologous end joining (NHEJ) are shared by a broad range of cells and organisms, some features are specific to individual situations. This article reviews the influence of repair mechanisms on the results of gene targeting experiments, with an emphasis on lessons learned from experiments with Drosophila.

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“…The different models reported in the literature are listed in Tables 1 and 2. It can be anticipated that recent methods for genome editing, such as the CRISPR/Cas9 system (Gratz et al, 2013) and TALENs technology enabling directed point mutations (Liu et al, 2012(Liu et al, , 2014Beumer and Carroll, 2014) will further facilitate generation of disease models reflecting the defects found in patients suffering from mitochondrial diseases even closer.…”

Section: Organelle Factsmentioning

confidence: 99%